Cell Surface Hydrophobicity and Adhesion: A Study on Fifty Clinical Isolates of Candida albicans

Raut, Jayant S.; Rathod, Vimal S.; Karuppayil, Sankunny Mohan

doi:10.3314/jjmm.51.131

Cited by 40 publications

(36 citation statements)

References 26 publications

(21 reference statements)

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“…Some previous studies described a positive correlation between hydrophobicity and adhesion to plastic and host cells while other reports couldn't find such relation (Panagoda et al, 2001; Gallardo-Moreno et al, 2002; Luo and Samaranayake, 2002; Samaranayake et al, 2003; Blanco et al, 2010; Raut et al, 2010; Yoshijima et al, 2010). We only found correlation between adhesion and hydrophobicity for C. parapsilosis strains.…”

Section: Discussionmentioning

confidence: 94%

Adhesion, biofilm formation, cell surface hydrophobicity, and antifungal planktonic susceptibility: relationship among Candida spp.

Silva-Dias¹,

et al. 2015

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We have performed the characterization of the adhesion profile, biofilm formation, cell surface hydrophobicity (CSH) and antifungal susceptibility of 184 Candida clinical isolates obtained from different human reservoirs. Adhesion was quantified using a flow cytometric assay and biofilm formation was evaluated using two methodologies: XTT and crystal violet assay. CSH was quantified with the microbial adhesion to hydrocarbons test while planktonic susceptibility was assessed accordingly the CLSI protocol for yeast M27-A3 S4. Yeast cells of non-albicans species exhibit increased ability to adhere and form biofilm. However, the correlation between adhesion and biofilm formation varied according to species and also with the methodology used for biofilm assessment. No association was found between strain's site of isolation or planktonic antifungal susceptibility and adhesion or biofilm formation. Finally CSH seemed to be a good predictor for biofilm formation but not for adhesion. Despite the marked variability registered intra and inter species, C. tropicalis and C. parapsilosis were the species exhibiting high adhesion profile. C. tropicalis, C. guilliermondii, and C. krusei revealed higher biofilm formation values in terms of biomass. C. parapsilosis was the species with lower biofilm metabolic activity.

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Section: Discussionmentioning

confidence: 94%

Adhesion, biofilm formation, cell surface hydrophobicity, and antifungal planktonic susceptibility: relationship among Candida spp.

Silva-Dias¹,

et al. 2015

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“…Exchanging molecular signals or using their own metabolic products are the mechanisms typically used by microorganisms to interact in the oral cavity . Candidal adherence to denture material increases when there is an increase in surface hydrophobicity …”

mentioning

confidence: 99%

Nicotine Upregulates Coaggregation of Candida albicans and Streptococcus mutans

Ashkanane

Gomez

Levon

et al. 2017

Journal of Prosthodontics

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“…We could show increased SC5314 cell hydrophobicity, indicating a shift to a CSH phenotype. It has to be taken into account that a recent report failed to correlate the hydrophobicity of 50 clinical C. albicans isolates with their adhesiveness to polystyrene 35 . Other reports however, have shown hydrophobicity not only to correlate with Candida adhesiveness but to be part of concerted pathogenicity factor expression 23,36 .…”

Section: Discussionmentioning

confidence: 99%

Rifampicin induced virulence determinants increase Candida albicans biofilm formation

et al. 2013

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Discuss this article AbstractIncreased intravenous catheter use has been paralleled by increased bacterial and yeast bloodstream infection. Biofilm formation, which is associated with the cell surface hydrophobicity (CSH) phenotype, represents a major pathogenicity strategy of , becoming especially important in the colonization Candida albicans of intravascular medical devices. Increasing evidence shows the induction of virulence factors in by diverse substances. Therefore, we C. albicans investigated whether rifampicin, an antibiotic shown to be capable of inducing MDR1 expression in may also promote the formation of a C. albicans pathogenic biofilm. In response to 40 µg/mL rifampicin, an enhanced retention of SC5314 cells on polystyrene culture plates was observed by C. albicans measuring increased metabolic activity by XTT assay, indicating induction of biofilm formation. Rifampicin treatment also induced fibronectin binding, cell hydrophobicity and germ tube formation. Furthermore, increased RNA and protein expression of CSH1p, a major mediator of CSH, was demonstrated. We conclude that exposure to rifampicin may result in upregulation of key Candida virulence determinants, potentially boosting pathogenicity and supporting biofilm formation. This finding gains clinical significance from the increasing popularity of rifampicin-coated catheters, which might provide an advantageous gateway for bloodstream infections.

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Cell Surface Hydrophobicity and Adhesion: A Study on Fifty Clinical Isolates of Candida albicans

Cited by 40 publications

References 26 publications

Adhesion, biofilm formation, cell surface hydrophobicity, and antifungal planktonic susceptibility: relationship among Candida spp.

Adhesion, biofilm formation, cell surface hydrophobicity, and antifungal planktonic susceptibility: relationship among Candida spp.

Nicotine Upregulates Coaggregation of Candida albicans and Streptococcus mutans

Rifampicin induced virulence determinants increase Candida albicans biofilm formation

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