2012
DOI: 10.1152/ajpheart.01218.2010
|View full text |Cite
|
Sign up to set email alerts
|

Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance

Abstract: McCain ML, Desplantez T, Geisse NA, Rothen-Rutishauser B, Oberer H, Parker KK, Kleber AG. Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance. Am J Physiol Heart Circ Physiol 302: H443-H450, 2012. First published November, 11, 2011 doi:10.1152/ajpheart.01218.2010.-Gap junctions are composed of connexin (Cx) proteins, which mediate intercellular communication. Cx43 is the dominant Cx in ventricular myocar… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
64
0

Year Published

2012
2012
2016
2016

Publication Types

Select...
7
2

Relationship

4
5

Authors

Journals

citations
Cited by 58 publications
(64 citation statements)
references
References 35 publications
0
64
0
Order By: Relevance
“…S2 A-C) and detected Cx43 immunosignal in punctate clusters along the cell-cell interface (Fig. S2 D and E), demonstrating that μtissues had electrical continuity via gap junction channels (39). These results suggest that, between Day 1 and 4, myocytes become synchronous and transmit force across the cell-cell junction instead of to the ECM near the cellcell interface, indicative of focal adhesion disassembly and intercalated disc maturation.…”
mentioning
confidence: 80%
“…S2 A-C) and detected Cx43 immunosignal in punctate clusters along the cell-cell interface (Fig. S2 D and E), demonstrating that μtissues had electrical continuity via gap junction channels (39). These results suggest that, between Day 1 and 4, myocytes become synchronous and transmit force across the cell-cell junction instead of to the ECM near the cellcell interface, indicative of focal adhesion disassembly and intercalated disc maturation.…”
mentioning
confidence: 80%
“…45 The present study of atrial myocardium, in which there is the added complexity of 2 coexpressed dominant connexins, Cx40 and Cx43, provides unique insight into human connexin biology, in particular the importance of Cx40 in determining atrial resistivity, and the effects of aging on atrial connexin expression and resistivity. Our results may also explain the finding that increased right atrial Cx40 expression in sinus rhythm is a predictor of the risk of developing postoperative AF, 23 and that an upregulation of right atrial Cx40 has been described in association with atrial fibrillation in man.…”
Section: Insights From Correlations Of Connexin Expression With Junctmentioning
confidence: 95%
“…To elucidate the effects of myocyte shape remodeling on electrical cell -cell coupling, we performed dual voltage clamp studies to correlate cell pair geometry to gap junction structure and function (McCain et al, 2012b). By seeding cardiac myocytes on micropatterned islands of fi bronectin, we controlled cell dimensions (length, height, and width) and junction geometry, as myocyte pairs consistently formed sigmoidal cell -cell junctions with relatively little variability in junction length or height.…”
Section: Engineering Cardiac Cell Pairs and The Intercellular Junctionmentioning
confidence: 99%
“…Figure 3A demonstrates that the ID between the two WT cells shows, as expected, immunofl uorescence signals for Cx43. Importantly, the engineered interface between a WT cell and a Cx43KO cell is negative for either Cx43 immunofl uorescence, indicating that gap junction channels are below immunodetection threshold (McCain et al, 2012b). Intercellular electrical conductance between these two types of cells is depicted in Figure 3C.…”
Section: Cx43mentioning
confidence: 99%