Cell Tracking Technologies for Acute Ischemic Brain Injury

Gavins, Felicity N. E.; Smith, Helen K.

doi:10.1038/jcbfm.2015.93

Cited by 16 publications

(22 citation statements)

References 107 publications

(123 reference statements)

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“…9,[21][22][23] Standardized protocols used for labeling stem cells with SPIONs were previously compiled by us, 15,24 and other direct-labeling reagents were reviewed by Marks and Nolan 25 and Progatzky et al 26 Indirect labeling is a considerably different method, which includes genetic modification in order to either produce an appropriate signal-generating molecule or increase the affinity of cells to contrast agents. 9,21,[27][28][29][30][31][32] Transient expression of reporter proteins by DNA vector transfection is often included in this set of cell labeling. 9 Another alternative is stable expression of the reporter protein by transduction of the cells with a virus.…”

Section: Labeling Stem Cells and Molecular Imaging Methodsmentioning

confidence: 99%

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Jasmin¹,

Souza²,

Louzada³

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) have been used for diagnoses in biomedical applications, due to their unique properties and their apparent safety for humans. In general, SPIONs do not seem to produce cell damage, although their long-term in vivo effects continue to be investigated. The possibility of efficiently labeling cells with these magnetic nanoparticles has stimulated their use to noninvasively track cells by magnetic resonance imaging after transplantation. SPIONs are attracting increasing attention and are one of the preferred methods for cell labeling and tracking in preclinical and clinical studies. For clinical protocol approval of magnetic-labeled cell tracking, it is essential to expand our knowledge of the time course of SPIONs after cell incorporation and transplantation. This review focuses on the recent advances in tracking SPION-labeled stem cells, analyzing the possibilities and limitations of their use, not only focusing on myocardial infarction but also discussing other models.

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Section: Labeling Stem Cells and Molecular Imaging Methodsmentioning

confidence: 99%

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Jasmin¹,

Souza²,

Louzada³

et al. 2017

IJN

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“…SPECT/CT is one of the most recommended imaging modalities for cell tracking (25,26). We observed that erythropoietin priming of ECFCs increased their ability to home to ischemic tissues and potentiated their beneficial effect on BBB disruption, brain apoptosis, and CBF, as all of these correlated significantly with ECFC homing intensity.…”

Section: Discussionmentioning

confidence: 71%

Erythropoietin Pretreatment of Transplanted Endothelial Colony-Forming Cells Enhances Recovery in a Cerebral Ischemia Model by Increasing Their Homing Ability: A SPECT/CT Study

et al. 2016

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Endothelial colony-forming cells (ECFCs) are promising candidates for cell therapy of ischemic diseases, as less than 10% of patients with an ischemic stroke are eligible for thrombolysis. We previously reported that erythropoietin priming of ECFCs increased their in vitro and in vivo angiogenic properties in mice with hindlimb ischemia. The present study used SPECT/CT to evaluate whether priming of ECFCs with erythropoietin could enhance their homing to the ischemic site after transient middle cerebral artery occlusion (MCAO) followed by reperfusion in rats and potentiate their protective or regenerative effect on blood-brain barrier (BBB) disruption, cerebral apoptosis, and cerebral blood flow (CBF). Methods: Rats underwent a 1-h MCAO followed by reperfusion and then 1 d after MCAO received an intravenous injection of either PBS (control, n 5 10), PBS-primed ECFCs (ECFC PBS , n 5 13), or erythropoietinprimed ECFCs (ECFC EPO , n 5 10). ECFC homing and the effect on BBB disruption, cerebral apoptosis, and CBF were evaluated by SPECT/CT up to 14 d after MCAO. The results were expressed as median 6 interquartile range for ipsilateral-to-contralateral ratio of the activity in middle cerebral artery-vascularized territories in each hemisphere. Histologic evaluation of neuronal survival and astrocytic proliferation was performed on day 14. Results: Erythropoietin priming increased homing of ECFCs to the ischemic hemisphere (ECFC PBS , 111.0% 6 16.0%; ECFC EPO , 146.5% 6 13.3%). BBB disruption was significantly reduced (control, 387% 6 153%; ECFC PBS , 151% 6 46% [P , 0.05]; ECFC EPO , 112% 6 9% [P , 0.001]) and correlated negatively with ECFC homing (Pearson r 5 20.6930, P 5 0.0002). Cerebral apoptosis was significantly reduced (control, 161% 6 10%; ECFC PBS , 141% 6 9% [P , 0.05]; ECFC EPO ,118% 6 5% [P , 0.001]) and correlated negatively with ECFC homing (r 5 20.7251, P , 0.0001). CBF was significantly restored with ECFCs and almost totally so with erythropoietin priming (control, 72% 6 2%; ECFC PBS , 90% 6 4% [P , 0.01]; ECFC EPO , 99% 6 4% [P , 0.001]) and correlated positively with ECFC homing (r 5 0.7348, P , 0.0001). Immunoblocking against the CD146 receptor on ECFCs highlighted its notable role in ECFC homing with erythropoietin priming (ECFC EPO , 147% 6 14%, n 5 4; ECFC EPO with antibody against CD146, 101% 6 12%, n 5 4 [P , 0.05]). Conclusion: Priming with erythropoietin before cell transplantation is an efficient strategy to amplify the migratory and engraftment capacities of ECFCs and their beneficial impact on BBB disruption, apoptosis, and CBF.

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“…Cell tracking is made feasible through bioluminescent optical imaging at a low cost, however, limited by the low penetration of light. In order to track cells deeper in tissue, positron emission tomography, single-photon emission computed tomography, and magnetic resonance imaging (MRI) can penetrate deeper within a subject utilizing a radioactive isotope (Gavins and Smith, 2015). MRI allows for imaging modalities to assess pathophysiological changes, outcomes of cerebral ischemia, vascular lesions, and blood flow.…”

Section: Perspective and Future Directionsmentioning

confidence: 99%

Stroke Repair via Biomimicry of the Subventricular Zone

Matta

Gonzalez

2018

Front. Mater.

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Stroke is among the leading causes of death and disability worldwide, 85% of which are ischemic. Current stroke therapies are limited by a narrow effective therapeutic time and fail to effectively complete the recovery of the damaged area. Magnetic resonance imaging of the subventricular zone (SVZ) following infarct/stroke has allowed visualization of new axonal connections and projections being formed, while new immature neurons migrate from the SVZ to the peri-infarct area. Such studies suggest that the SVZ is a primary source of regenerative cells for the repair and regeneration of stroke-damaged neurons and tissue. Therefore, the development of tissue engineered scaffolds that serve as a bioreplicative SVZ niche would support the survival of multiple cell types that reside in the SVZ. Essential to replication of the human SVZ microenvironment is the establishment of microvasculature that regulates both the healthy and stroke-injured blood-brain barrier, which is dysregulated poststroke. In order to reproduce this niche, understanding how cells interact in this environment is critical, in particular neural stem cells, endothelial cells, pericytes, ependymal cells, and microglia. Remodeling and repair of the matrix-rich SVZ niche by endogenous reparative mechanisms may then support functional recovery when enhanced by an artificial niche that supports the survival and proliferation of migrating vascular and neuronal cells. Critical considerations to mimic this area include an understanding of resident cell types, delivery method, and the use of biocompatible materials. Controlling stem cell survival, differentiation, and migration are key factors to consider when transplanting stem cells. Here, we discuss the role of the SVZ architecture and resident cells in the promotion and enhancement of endogenous repair mechanisms. We elucidate the interplay between the extracellular matrix composition and cell interactions prior to and following stroke. Finally, we review current cell and neuronal niche biomimetic materials that allow for a tissue-engineered approach in order to promote structural and functional restoration of neural circuitry. By creating an artificial mimetic SVZ, tissue engineers can strive to facilitate tissue regeneration and functional recovery.

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Cell Tracking Technologies for Acute Ischemic Brain Injury

Cited by 16 publications

References 107 publications

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Erythropoietin Pretreatment of Transplanted Endothelial Colony-Forming Cells Enhances Recovery in a Cerebral Ischemia Model by Increasing Their Homing Ability: A SPECT/CT Study

Stroke Repair via Biomimicry of the Subventricular Zone

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