1974
DOI: 10.1101/sqb.1974.039.01.054
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Cell Transformation: A Study of Regulation with Types 5 and 12 Adenovirus Temperature-sensitive Mutants

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1977
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Cited by 118 publications
(84 citation statements)
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“…Hence, the isolation and characterization of temperature-sensitive mutants of adenoviruses (7)(8)(9)(10) suggested that they, too, may be useful to identify the critical gene(s) involved in, and to detect the gene product(s) essential for, transformation. In contrast to our expectation from data obtained with Rous sarcoma (1) and SV40 (2) viruses, however, an adenovirus mutant defective in DNA replication (5), H5ts125, was discovered that transformed rat embryo cells at frequencies 3 to 8 times greater than wild-type 5 adenovirus at the nonpermissive (39.50) and semipermissive (36°) temperatures (11,12). This finding implies that a specific viral gene product affects transformation, perhaps by modulating against it, so that, when the viral protein is nonfunctional or functions aberrantly, transformation is less restricted.…”
contrasting
confidence: 54%
“…Hence, the isolation and characterization of temperature-sensitive mutants of adenoviruses (7)(8)(9)(10) suggested that they, too, may be useful to identify the critical gene(s) involved in, and to detect the gene product(s) essential for, transformation. In contrast to our expectation from data obtained with Rous sarcoma (1) and SV40 (2) viruses, however, an adenovirus mutant defective in DNA replication (5), H5ts125, was discovered that transformed rat embryo cells at frequencies 3 to 8 times greater than wild-type 5 adenovirus at the nonpermissive (39.50) and semipermissive (36°) temperatures (11,12). This finding implies that a specific viral gene product affects transformation, perhaps by modulating against it, so that, when the viral protein is nonfunctional or functions aberrantly, transformation is less restricted.…”
contrasting
confidence: 54%
“…However, ts mutants in viral DNA synthesis exist both for adenoviruses (13) and papovaviruses (14). Temperature-sensitive mutants in Ad DNA synthesis have been studied for Ad5 (15,16), Adl2 (17), and Ad31 (18). Thus far, three complementation groups have been defined among the various ts Ad DNA negative mutants.…”
mentioning
confidence: 99%
“…It also appears to be involved at some step in the establishment of cell transformation, since temperature-sensitive mutants of the DNA-binding protein transform rat cells in culture with a higher efficiency than wild-type virus [18]. Cells transformed by these mutants contain extensive regions of the viral genome integrated into their chromosomes, whereas wild-type transformants do not [19].…”
mentioning
confidence: 99%