“…When semipermissive rat embryo fibroblasts are transformed by adenovirus type 2 or 5 (Ad2 or Ad5), about 15% of the viral genome, representing the left end, usually persists in the transforned cell, although on occasion a larger proportion of the viral DNA is present (14,15,23,26). However, when cells are transformed with the DNA-binding protein mutant H5ts125, which transforms rat embryo fibroblasts with a threeto eight-times-higher frequency than wild-type Ad5 (Ad5 WT) at the nonpermissive (39.5°C) or semipernissive (3600) temperature (17), cells often contain one or more copies of the entire adenovirus genone (23). These findings, however, do not describe the physical state of the viral DNA in H5ts125-transformed cells.…”