Cell tropism and molecular epidemiology of Anaplasma platys-like strains in cats

Zobba, Rosanna; Anfossi, Antonio Giovanni; Visco, S.; Sotgiu, Francesca; Dedola, Carla; Parpaglia, Maria Luisa Pinna; Battilani, Mara; Pittau, Marco; Alberti, Alberto

doi:10.1016/j.ttbdis.2015.01.008

Cited by 38 publications

(32 citation statements)

References 22 publications

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“…In this study, randomly selected dromedaries did not show any symptoms specifically referable to A. platys infection. Therefore the A. platys-like strains isolated in camels might not be pathogenic and not cause any symptoms, as previously observed in ruminants from China and Italy [10,17] and in cats from Italy [18].…”

Section: Discussionsupporting

confidence: 55%

“…platys, the etiologic agent of canine infectious cyclic thrombocytopenia, has been associated with thrombocytopenia and anemia [17,18]. In this study, randomly selected dromedaries did not show any symptoms specifically referable to A. platys infection.…”

Section: Discussionmentioning

confidence: 72%

“…Overall, 66 out of 226 camels (29.2%) were seropositive. The discrepancy between molecular and serological tests could be explained by cross-reactivity of the antigen used in serology with anti-cytoplasmic antibodies, as well as with other autoimmune antibodies and/or with antibodies related to other Anaplasma species closely related to those of A. phagocytophilum [17,18,45]. Notably, previous studies reported a great degree of cross-reactivity in serological tests between Anaplasma species [46][47][48].…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, infection with A. centrale induces long-lasting protective immunity in In Sicily, Italy, strains closely related to A. platys have been detected in neutrophils of cattle, sheep and goats [17] and in platelets of cats [18]. Based on genetic analyses using 16S rRNA and groEL genes, these strains revealed very high levels of nucleotide identity with canine A. platys strains (99% and 92%-93% identities with A. platys 16S rRNA and groEL genes, respectively) and were placed in a distinct monophyletic cluster closely related to A. platys sequences [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)

Belkahia

Saïd

Sayahi³

et al. 2015

J Infect Dev Ctries

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Introduction: Little information is currently available regarding the presence of Anaplasma species in North African dromedaries. To fill this gap in knowledge, the prevalence, risk factors, and genetic diversity of Anaplasma species were investigated in Tunisian dromedary camels. Methodology: A total of 226 camels from three different bioclimatic areas were sampled and tested for the presence of Anaplasma species by quantitative polymerase chain reaction (qPCR) and nested polymerase chain reaction (nPCR) assays. Detected Anaplasma strains were characterized by 16S rRNA sequence analysis. Results: Overall infection rate of Anaplasma spp. was 17.7%, and was significantly higher in females. Notably, A. marginale, A. centrale, A. bovis, and A. phagocytophilum were not detected. Animals were severely infested by three tick species belonging to the genus Hyalomma (H. dromedarii, H. impeltatum, and H. excavatum). Alignment, similarity comparison, and phylogenetic analysis of the 16S rRNA sequence variants obtained in this study suggest that Tunisian dromedaries are infected by more than one novel Anaplasma strain genetically related to A. platys. Conclusions: This study reports the presence of novel Anaplasma sp. strains genetically related to A. platys in dromedaries from various bioclimatic areas of Tunisia. Findings raise new concerns about the specificity of the direct and indirect diagnostic tests routinely used to detect different Anaplasma species in ruminants and provide useful molecular information to elucidate the evolutionary history of bacterial species related to A. platys.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)

Belkahia

Saïd

Sayahi³

et al. 2015

J Infect Dev Ctries

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“…Owing to the higher number of newly obtained sequences in this study and higher variability of the gene, more subclades within the A. platys group were seen in the latter analyses. Moreover, new A. platys and A. platys -like isolates from Italy [23] were found to be in a unique subclade that appears to cluster differently from the detected Philippine A. platys variants. In this study, groEL was used to show the different variants of A. platys because of its higher intra- and inter-species variation than the 16S rRNA [13,18].…”

Section: Resultsmentioning

confidence: 99%

Multiple infections of Anaplasma platys variants in Philippine dogs

Ybañez

Yokoyama

et al. 2016

Vet World

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Aim:Anaplasma platys, the causative agent of infectious canine cyclic thrombocytopenia, is a tick-borne pathogen that also has been implicated as potentially zoonotic. To provide molecular evidence on the multiple infections of A. platys variants in Philippine dogs.Materials and Methods:DNA fragments of A. platys from infected dogs in the Philippines were molecularly characterized. For screening, 25 dogs suspected to have canine anaplasmosis were tested using a 16S rRNA-based nested polymerase chain reaction (PCR). Infection was confirmed by sequencing of positive amplicons. Second round PCR targeting a longer 16S rRNA fragment was subsequently performed on the first round PCR amplicons of the positive samples. Further characterization using the heat-shock operon (groEL) gene was also performed on the A. platys-positive samples.Results:10 16S rRNA sequences were obtained and found 99.6-100% identical to each other and 99.6-99.7% identical to the closest registered A. platys sequences. On the other hand, 36 groEL clone sequences were obtained and found to be 85.1-99.8% identical with each other and 85.0-88.9% identical to the closest previously registered A. platys sequences. Four dogs were found coinfected with 2-3 groEL variant sequences. Phylogenetic analyses suggest that the detected A. platys in the Philippines may represent unique variants.Conclusion:A. platys variants were detected in Philippine dogs. Coinfection of different A. platys variants in dogs was also demonstrated. The present study may indicate the potential genetic diversity of A. platys in the country.

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Molecular detection and groEL typing of Rickettsia aeschlimannii in Sardinian ticks

et al. 2016

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Rickettsia aeschlimannii is an emerging tick-borne pathogen of the spotted fever group Rickettsiae with considerable impact on both human and animal health. This study reports the molecular detection and groEL characterization of R. aeschlimannii in ticks collected from birds and ruminants in a typical Mediterranean environment. Phylogeny of R. aeschlimannii and species representative of the spotted fever and typhus groups based on the groEL gene is reconstructed for the first time. Results expand the knowledge on distribution and typing of emerging human tick-borne diseases in Sardinia and pave the way for future molecular epidemiology studies of zoonotic Rickettsiae.

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Cell tropism and molecular epidemiology of Anaplasma platys-like strains in cats

Cited by 38 publications

References 22 publications

Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)

Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)

Multiple infections of Anaplasma platys variants in Philippine dogs

Molecular detection and groEL typing of Rickettsia aeschlimannii in Sardinian ticks

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