Cell-Type-Specific Expression of the TFIID Component TAFII135 in the Nervous System

Metsis, Madis; Brunkhorst, Adrian; Neuman, Toomas

doi:10.1006/excr.2001.5307

Cited by 9 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such factors may interact directly with sequences in the TATA-box or do so indirectly through components of chromatin and complexes like TFIID (Martinez, 2002). Some of the known cell-specific TAFs reported from nonplant systems are hTAFII105, which expresses at a higher level in B cells (Dikstein et al, 1996), although its function is not clear (Freiman et al, 2002); TAFII135 in neuronal differentiation (Metsis et al, 2001); and the cell type-and stage-specific TRF2 in mouse spermatogenesis (Zhang et al, 2001). In the case of plant cells, Pan et al (2000) reported that activation of promoters driven by a single activation element (and not complex promoters with multiple cis-motifs) requires functional interaction between the cognate transcription factor and the PIC.…”

Section: Discussionmentioning

confidence: 99%

The TATA-Box Sequence in the Basal Promoter Contributes to Determining Light-Dependent Gene Expression in Plants

et al. 2006

View full text Add to dashboard Cite

A prototype 13-bp TATA-box sequence, TCACTATATATAG, was mutated at each nucleotide position and examined for its function in the core promoter. Specific nucleotides in the first TATA, the second TATA, as well as the flanking sequences influenced promoter function in transient transformation of tobacco (Nicotiana tabacum var Petit Havana) leaves. The effect of a given mutation on reporter gene expression in light versus dark was variable and sometimes contrasting. Some mutations, like T 7 or A 8 /C or G, completely inactivated the expression of the minimal promoter in light but not in dark. In general, the sequence requirement for dark expression was less stringent than that for light expression. The selective effect of TATA-box mutations on light versus dark expression was exerted on core promoter function in the chromatin-integrated state also. Even in the presence of an upstream light response activator element, TATA-box mutations influenced modulation of the promoter by light. An A at the eighth position was specifically involved in the red light response of the promoter. Selectivity in gene expression was associated with a high level of transcript initiation from a site that was not active in the dark. Nuclear proteins from dark-and light-grown seedlings showed that the sequence variation within the TATA-box governs the formation of alternative transcriptional complexes. The experiments give direct evidence for the role of a core TATA-box sequence in determining the level as well as selectivity of gene expression in plants.

show abstract

Section: Discussionmentioning

confidence: 99%

The TATA-Box Sequence in the Basal Promoter Contributes to Determining Light-Dependent Gene Expression in Plants

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Transient transfections of NSC were performed essentially according to the supplier's recommendations (Effectene, Qiagen). Expression constructs used in this study: pCMV-TAF4 (Metsis, Brunkhorst et al 2001) corresponding to aa 42-702 of the 702 amino acid annotated mouse protein (XP130764); pCMV-TAF4del harboring a deletion of the Cterminus (aa 338-702); pCMV-HA-RanBPM (Mikolajczyk, Shi et al 2003); pCMVEGFP.…”

Section: Dna Delivery and Constructsmentioning

confidence: 99%

“…In situ hybridization was performed by standard methods as described earlier (Metsis, Brunkhorst et al 2001). Briefly, 14 mm sections were cut from fresh-frozen tissue, mounted on glass slides, and postfixated with 4% paraformaldehyde for 20 min.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…Briefly, 14 mm sections were cut from fresh-frozen tissue, mounted on glass slides, and postfixated with 4% paraformaldehyde for 20 min. Hybridization was performed for 16 hrs at 42°C with hybridization buffer containing 2.5x106cpm/ml [35S]UTP labeled riboprobe (Metsis, Brunkhorst et al 2001). After hybridization, the slides were RNAse treated and washed in a series of SSC rinses at decreasing concentrations with a final concentration of 0.1X SSC at 65°C.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

See 1 more Smart Citation

Novel isoforms of the TFIID subunit TAF4 modulate nuclear receptor-mediated transcriptional activity

Brunkhorst

Neuman

Hall

et al. 2004

Biochemical and Biophysical Research Communications

Self Cite

View full text Add to dashboard Cite

“…TAF4 and TAF4b are required for embryogenesis in flies and mammals, as well as participate in gamete production (Metzger et al, 1999;Bell et al, 2001;Freiman et al, 2001;Guermah et al, 2003;Hiller et al, 2004). TAF4 is also required for activation of the master regulators Dorsal and Twister in Drosophila (Zhou et al, 1998;Pham et al, 1999) and is critical to neuronal cell differentiation of the embryonic brain and spinal cord in mice (Metsis et al, 2001).…”

Section: Role Of Tfiid In Developmentmentioning

confidence: 99%

General Transcription Factors and the Core Promoter: Ancient Roots

Gurley¹,

O’Grady²,

Czarnecka‐Verner³

et al.

Regulation of Transcription in Plants

View full text Add to dashboard Cite

Cell-Type-Specific Expression of the TFIID Component TAFII135 in the Nervous System

Cited by 9 publications

References 36 publications

The TATA-Box Sequence in the Basal Promoter Contributes to Determining Light-Dependent Gene Expression in Plants

The TATA-Box Sequence in the Basal Promoter Contributes to Determining Light-Dependent Gene Expression in Plants

Novel isoforms of the TFIID subunit TAF4 modulate nuclear receptor-mediated transcriptional activity

General Transcription Factors and the Core Promoter: Ancient Roots

Contact Info

Product

Resources

About