Cell vesiculation and immunopathology: implications in cerebral malaria

Coltel, Nicolas; Combes, Valéry; Wassmer, Samuel C.; Chimini, Giovanna; Grau, Georges E.

doi:10.1016/j.micinf.2006.04.006

Cited by 65 publications

(63 citation statements)

References 152 publications

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“…It seems that we may be underestimating the extent of P-gp transfer. Although FCM is the method of choice for studying MPs, 21,42 the level of detection is dependent on size, level of expression and distribution of the antigen on the surface. In the case of MDR þ MP, immunodetection of P-gp by FCM may be limited in this aspect.…”

Section: Discussionmentioning

confidence: 99%

“…21,22 MP are released from the plasma membranes of various cell types, including platelets, macrophages, monocytes, T-cells, endothelial cells and erythrocytes. 23 Once considered 'inert side-products' of cellular activation, MP have become important mediators of inflammation, coagulation and vascular homeostasis.…”

Section: Introductionmentioning

confidence: 99%

“…24 MP are released upon cellular activation or during late apoptosis after a loss of membrane asymmetry and remodeling of the cytoskeleton. 21 MP carry surface antigens, proteins and adhesion molecules from their originating cell 25 and can mediate intercellular cross-talk by transferring receptors, antigens and cytokines from donor to recipient cells. 20,21 The presence of cell adhesion molecules on MP supports a capacity for targeted cell binding and cell-cell interactions.…”

Section: Introductionmentioning

confidence: 99%

“…21 MP carry surface antigens, proteins and adhesion molecules from their originating cell 25 and can mediate intercellular cross-talk by transferring receptors, antigens and cytokines from donor to recipient cells. 20,21 The presence of cell adhesion molecules on MP supports a capacity for targeted cell binding and cell-cell interactions. 25 Here we show for the first time that purified MP transfer functional P-gp from resistant cancer cells to drug-sensitive cells in vitro and discuss the clinical implications of these findings in the emergence and treatment of acquired MDR in cancer.…”

Section: Introductionmentioning

confidence: 99%

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Membrane microparticles mediate transfer of P-glycoprotein to drug sensitive cancer cells

et al. 2009

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Multidrug resistance (MDR), a significant impediment to the successful treatment of cancer clinically, has been attributed to the overexpression of P-glycoprotein (P-gp), a plasma membrane multidrug efflux transporter. P-gp maintains sublethal intracellular drug concentrations by virtue of its drug efflux capacity. The cellular regulation of P-gp expression is currently known to occur at either pre-or post-transcriptional levels. In this study, we identify a 'non-genetic' mechanism whereby microparticles (MPs) serve as vectors in the acquisition and spread of MDR. MPs isolated from drug-resistant cancer cells (VLB 100 ) were co-cultured with drug sensitive cells (CCRF-CEM) over a 4 h period to allow for MP binding and P-gp transfer. Presence of P-gp on MPs was established using flow cytometry (FCM) and western blotting. Whole-cell drug accumulation assays using rhodamine 123 and daunorubicin (DNR) were carried out to validate the transfer of functional P-gp after co-culture. We establish that MPs shed in vitro from drug-resistant cancer cells incorporate cell surface P-gp from their donor cells, effectively bind to drug-sensitive recipient cells and transfer functional P-gp to the latter. These findings serve to substantially advance our understanding of the molecular basis for the emergence of MDR in cancer clinically and lead to new treatment strategies which target and inhibit MP mediated transfer of P-gp during the course of treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Membrane microparticles mediate transfer of P-glycoprotein to drug sensitive cancer cells

et al. 2009

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“…Nonetheless, it has recently been postulated that platelets and platelet and endothelial cell derived microparticles -submicron particles generated by vesiculation of cellular membranes (reviewed by Coltel et al 2006) -may bind to brain endothelial cells, providing a source of CD36 that allows indirect CD36-mediated pRBC binding to brain endothelial cells (Wassmer et al 2004 ;Faille et al 2009); however, this hypothesis remains to be validated in vivo.…”

Section: T H E R O L E O F P a R A S I T E S E Q U E S T R A T I Omentioning

confidence: 99%

Cerebral malaria: why experimental murine models are required to understand the pathogenesis of disease

et al. 2009

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Cerebral malaria is a life-threatening complication of malaria infection. The pathogenesis of cerebral malaria is poorly defined and progress in understanding the condition is severely hampered by the inability to study in detail, ante-mortem, the parasitological and immunological events within the brain that lead to the onset of clinical symptoms. Experimental murine models have been used to investigate the sequence of events that lead to cerebral malaria, but there is significant debate on the merits of these models and whether their study is relevant to human disease. Here we review the current understanding of the parasitological and immunological events leading to human and experimental cerebral malaria, and explain why we believe that studies with experimental models of CM are crucial to define the pathogenesis of the condition.

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Potential of extracellular vesicles in the pathogenesis, diagnosis and therapy for parasitic diseases

Pinheiro,

Torrecilhas,

Souza

et al. 2024

J of Extracellular Vesicle

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Parasitic diseases have a significant impact on human and animal health, representing a major hazard to the public and causing economic and health damage worldwide. Extracellular vesicles (EVs) have long been recognized as diagnostic and therapeutic tools but are now also known to be implicated in the natural history of parasitic diseases and host immune response modulation. Studies have shown that EVs play a role in parasitic disease development by interacting with parasites and communicating with other types of cells. This review highlights the most recent research on EVs and their role in several aspects of parasite‐host interactions in five key parasitic diseases: Chagas disease, malaria, toxoplasmosis, leishmaniasis and helminthiases. We also discuss the potential use of EVs as diagnostic tools or treatment options for these infectious diseases.

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Cell vesiculation and immunopathology: implications in cerebral malaria

Cited by 65 publications

References 152 publications

Membrane microparticles mediate transfer of P-glycoprotein to drug sensitive cancer cells

Membrane microparticles mediate transfer of P-glycoprotein to drug sensitive cancer cells

Cerebral malaria: why experimental murine models are required to understand the pathogenesis of disease

Potential of extracellular vesicles in the pathogenesis, diagnosis and therapy for parasitic diseases

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