Cell viability and proliferation capability of long-term human dental pulp stem cell cultures

Martín-Piedra, Miguel Ángel; Garzón, Ingrid; Oliveira, Ana Celeste; Alfonso, Camilo; Carriel, Víctor; Alaminos, Miguel

doi:10.1016/j.jcyt.2013.10.016

Cited by 59 publications

(61 citation statements)

References 31 publications

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“…Initially, that fact would contraindicate the transplantation of MSCs. However, the cell viability of the cultures, which was evaluated using the Trypan Blue method that is extensively used among studies on MSCs (Martin-Piedra et al, 2013, 2014Reissis et al, 2013), was approximately 96%. Furthermore, no statistically significant differences were observed between the two passages when measuring the tail length.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

et al. 2014

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ABSTRACT. Rupture of the Achilles tendon diminishes quality of life. The gold-standard therapy is a surgical suture, but this presents complications, including wound formation and inflammation. These complications spurred evaluation of the therapeutic potential of 10435 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 10434-10449 (2014) Stem cells for tendon injury treatment mesenchymal stem cells (MSCs) from adipose tissue. New Zealand rabbits were divided into 6 groups (three treatments with two time points each) evaluated at either 14 or 28 days after surgery: cross section of the Achilles tendon (CSAT); CSAT + Suture; and CSAT + MSC. A comparison between all groups at both time points showed a statistically significant increase in capillaries and in the structural organization of collagen in the healed tendon in the CSAT + Suture and CSAT + MSC groups at the 14-day assessment. Comparison between the two time points within the same group showed a statistically significant decrease in the inflammatory process and an increase in the structural organization of collagen in the CSAT and CSAT + MSC groups. A study of the genomic integrity of the cells suggested a linear correlation between an increase of injuries and culture time. Thus, MSC transplantation is a good alternative for treatment of Achilles tendon ruptures because it may be conducted without surgery and tendon suture and, therefore, has no risk of adverse effects resulting from the surgical wound or inflammation caused by nonabsorbable sutures. Furthermore, this alternative treatment exhibits a better capacity for wound healing and maintaining the original tendon architecture, depending on the arrangement of the collagen fibers, and has important therapeutic potential.

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Section: Discussionmentioning

confidence: 99%

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

et al. 2014

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“…This implies that cell viability can be affected by several factors, and that the contamination of cells by pathogens (like bacteria) and other in situ/in vitro debri, is perhaps a major factor that hinders the growth of healthy cells in the laboratory. The introduction of mitogens in tissue culture flasks is known to stimulate the viability of cells, however, there has been a few reports in which plant compounds and extracts have a cell proliferative effect (Majno and Joris, 1995;Vab Cruchten and Van Den Broeck, 2002;Lee et al, 2004;Goldtein and Kroemer, 2006). Obtaining the viability cell count (with, without, during, after or before treatment), with emphasis on mammalian cells such as fibroblasts, cancer cells, leukocytes, red blood cells etc., usually involves using the Trypan Blue method (Martin-Piedra et al, 2004).…”

Section: The Concept Of Cell Viabilitymentioning

confidence: 99%

“…The introduction of mitogens in tissue culture flasks is known to stimulate the viability of cells, however, there has been a few reports in which plant compounds and extracts have a cell proliferative effect (Majno and Joris, 1995;Vab Cruchten and Van Den Broeck, 2002;Lee et al, 2004;Goldtein and Kroemer, 2006). Obtaining the viability cell count (with, without, during, after or before treatment), with emphasis on mammalian cells such as fibroblasts, cancer cells, leukocytes, red blood cells etc., usually involves using the Trypan Blue method (Martin-Piedra et al, 2004). The method ensures that only viable cells are counted, but simultaneously allows for the investigation to view, unstained, dead cells in tissue culture flasks (Martin-Piedra et al, 2004).…”

Section: The Concept Of Cell Viabilitymentioning

confidence: 99%

“…Obtaining the viability cell count (with, without, during, after or before treatment), with emphasis on mammalian cells such as fibroblasts, cancer cells, leukocytes, red blood cells etc., usually involves using the Trypan Blue method (Martin-Piedra et al, 2004). The method ensures that only viable cells are counted, but simultaneously allows for the investigation to view, unstained, dead cells in tissue culture flasks (Martin-Piedra et al, 2004). This method of obtaining the number of living (also dead) cells can become labourintensive, because sometimes the cells appear to be clumped when viewed under the haemocytometer.…”

Section: The Concept Of Cell Viabilitymentioning

confidence: 99%

“…The ideal method of culturing eukaryote and prokaryote cells differ greatly, though, the one thing we can be sure of, is they both can be maintained in tissue culture systems. These systems involve culture media that are normally preselected, based on the individual requirements that a cell needs to grow optimally in its natural environment (Martin-Piedra et al, 2004). This, in essence, means that eukaryote and prokaryote cells are in constant contact with selective pressures in the environment.…”

Section: Introductionmentioning

confidence: 99%

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Three crucial biological characteristics that’s required for sustaining the livelihood of prokaryote and eukaryote cells

Singh¹

2017

Braz. J. Biol. Sci.

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Abstract. Since living organisms will always form an important part of the ecosystem, it's imperative to achieve a view on how imbalances cause problems. The one way in attaining this view is by understanding the way in which cells behave in different environments, ranging from the native environment to tested conditions. Cell viability, respiratory events as well as metabolic and nuclear shutdown are some critical events that occur, generally, in prokaryote and eukaryote cells. The distinct characteristics between the two cell types enable an advanced understanding about the occurrence of those features and processes attained during medicinal, and other cell-based studies.

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In vitrocharacterization of a nanostructured fibrin agarose bio-artificial nerve substitute

Carriel

Scionti

Campos

et al. 2015

J Tissue Eng Regen Med

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Neural tissue engineering is focused on the design of novel biocompatible substitutes to repair peripheral nerve injuries. In this paper we describe a nanostructured fibrin-agarose bioartificial nerve substitute (NFABNS), based on nanostructured fibrin-agarose hydrogels (FAHs) with human adipose-derived mesenchymal stem cells (HADMSCs). These NFABNSs were mechanically characterized and HADMSCs behaviour was evaluated using histological and ultrastructural techniques. Mechanical characterization showed that the NFABNSs were resistant, flexible and elastic, with a high deformation capability. Histological analyses carried out in vitro during 16 days revealed that the number of HADMSCs decreased over time, with a significant increase after 16 days. HADMSCs formed cell clusters and degraded the surrounding scaffold during this time; additionally, HADMSCs showed active cell proliferation and cytoskeletal remodelling, with a progressive synthesis of extracellular matrix molecules. Finally, this study demonstrated that it is possible to generate biologically active and mechanically stable tissue-like substitutes with specific dimensions, based on the use of HADMSCs, FAHs and a nanostructure technique. However, in vivo analyses are needed to demonstrate their potential usefulness in peripheral nerve repair. Copyright © 2015 John Wiley & Sons, Ltd.

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Cell viability and proliferation capability of long-term human dental pulp stem cell cultures

Cited by 59 publications

References 31 publications

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

Three crucial biological characteristics that’s required for sustaining the livelihood of prokaryote and eukaryote cells

In vitrocharacterization of a nanostructured fibrin agarose bio-artificial nerve substitute

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