Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models

Bonnier, Franck; Keating, Mark E.; Wróbel, Tomasz; Majzner, Katarzyna; Barańska, Małgorzata; García-Muñoz, Amaya; Blanco, Alfonso; Byrne, Hugh J.

doi:10.1016/j.tiv.2014.09.014

Cited by 209 publications

(180 citation statements)

References 37 publications

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“…In short term cell cultures (up to 5 days) cell viability is the same in 2D and in 3D growth conditions. In long term cell cultures cell viability in 3D conditions were slightly reduced probably due to the different exposure of cell area to the environment and dye uptake (16). To analyse early drug-drug interaction, human hepatocytes are the recommended in vitro assay system.…”

Section: Applications Of 3d Cell Culture In Drug Discoverymentioning

confidence: 99%

Three-dimensional cell cultures as a new tool in drug discovery

et al. 2016

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in 3D format will be shown. Conclusions: Although biological significance of obtained data from 2D and 3D cell cultures is still poorly understood, discrepancy of compunds activity illustrated importance of implementation 3D cell culture assays in early part of drug discovery process.IntroductIon T he costs for successfully introduction a NCE (new chemical entity) drug compound to the market is between $800 million and $1,2 billion, with an average duration of about 10 -15 years. Expenses are even much higher in the case of late stage failure (or failure of already marketed drug) (1). Analyses of clinical trials reveal that about 67% of drug lead molecules fail in late clinical trials stages, most of them due to poor efficacy and safety issues. This high attrition rate indicate that current pre-clinical screening and in vitro models do not adequately provide critical information required for prediction of efficacy and safety issues, which remain the main reasons for drug failure (2). The ability of an assay to produce reliable information about tested NCEs is essential in drug development. Therefore, one way to improve drug dis-

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Section: Applications Of 3d Cell Culture In Drug Discoverymentioning

confidence: 99%

Three-dimensional cell cultures as a new tool in drug discovery

et al. 2016

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“…This includes more representative culture models which better mimic the in vivo environment as well as more rapid, cost efficient, high content, and ideally label free screening technologies. It is crucial, however, that these models and technologies are well validated against established gold standards ref 33,34 .…”

Section: Page 20 Of 29 Analystmentioning

confidence: 99%

Multivariate statistical methodologies applied in biomedical Raman spectroscopy: assessing the validity of partial least squares regression using simulated model datasets

Keating

Nawaz

Bonnier

et al. 2015

Analyst

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Raman spectroscopy is fast becoming a valuable analytical tool in a number of biomedical scenarios, most notably disease diagnostics. Importantly, the technique has also shown increasing promise in the assessment of drug interactions on a cellular and subcellular level, particularly when coupled with multivariate statistical analysis. However, with respect to both Raman spectroscopy and the associated statistical methodologies, an important consideration is the accuracy of these techniques and more specifically the sensitivities which can be achieved, and ultimately the limits of detection of the various methods. The purpose of this study is thus the construction of a model simulated data set with the aim of testing the accuracy and sensitivity of the partial least squares regression

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“…It is one of the most highly referenced compounds used for viability and cytotoxicity assays. Generally, its use has been carried out to various aspects of monitoring cellular health [8,9], cytotoxicity [10], test compound toxicology in environmental risk assessments and in medicine [6], and antimicrobial susceptibility testing [11].…”

Section: Introductionmentioning

confidence: 99%

Simplification and sensitivity study of Alamar Blue bioassay for toxicity assessment in liquid media

Zaré

Amin

Nikaeen

et al. 2016

Desalination and Water Treatment

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A B S T R A C TBacteria, as an essential component of most wastewater treatment plants (WWTPs), play a critical role in the biological stage of WWTPs due to their role in cycling mineral compounds and in the decomposition of organic material. In this study, a modified Alamar Blue-based bioassay was used to evaluate the toxicity of metal plating wastewaters on sequence batch reactor (SBR) bacteria. For confirming the robustness, reproducibility, and efficiency of the optimized method, it was compared with the standard colony count protocol and oxygen consumption method. The influence of pH on the Alamar Blue reduction was examined over a pH range of 1.0-13.0. The optimum pH for Alamar Blue bioassay using SBR bacteria was 6.5-7. The correlation between the reduction of Alamar Blue by SBR bacteria and their oxygen consumption coefficient was 0.945. According to our optimized bioassay, Alamar Blue was rapidly reduced by active SBR bacteria. This protocol successfully assessed the toxicity of metal plating wastewaters. As most of the WWTPs are inefficient for elimination of toxic effect of metals, it is desirable to use bioassays that can determine the toxicity of such pollutants. We simplified and optimized such an assay that can be manipulated for real metal plating wastewater samples.

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Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models

Cited by 209 publications

References 37 publications

Three-dimensional cell cultures as a new tool in drug discovery

Three-dimensional cell cultures as a new tool in drug discovery

Multivariate statistical methodologies applied in biomedical Raman spectroscopy: assessing the validity of partial least squares regression using simulated model datasets

Simplification and sensitivity study of Alamar Blue bioassay for toxicity assessment in liquid media

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