Cell Wall Assembly in Fungal Morphogenesis

Sietsma, J. H.; Wessels, J. G. H.

doi:10.1007/978-3-642-76074-7_7

Cited by 9 publications

(5 citation statements)

References 57 publications

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“…Similarly, J-4, J-12 and J-13 which were potent producers of chitinase were displaying antifungal activity against A. fumigatus (17.80 and 18.41% respectively). The antifungal activity of J-7 can be attributed to its glucanase release potential, since β-1, 3-glucans are major constituents of many fungal cell walls (Sietsma and Wessels, 1979). Various workers have confirmed in vitro lysis of fungal cell walls either by bacterial chitinases or glucanases alone or by a mixture of these enzymes (Ordentlich et al, 1988;Fiske et al, 1990;Ashokvardhan et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens

Saini

Gangwar

Kalia

et al. 2016

JANS

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Isolation of endophytic actinomycetes is an important step to screen antimicrobial compounds to curb the threat of drug-resistant strains of human pathogens. Out of the 50 endophytic actinomycetes obtained from surface sterilized root, stem and leaf tissues of Syzygium cumini, 50 isolates (30%) exhibited antimicrobial activity. Antistaphylococcal activity was displayed by most of the isolates, with maximum percent inhibition by J-10 (Mean of Inhibition Factor=12.12 mm2). A total of 8 isolates (4 each) were able to hydrolyse protein (proteinase activity) and solubilize chitin (chitinase activity). Results of thin layer chromatography confirm the production of chloramphenicol family |antibiotic by the isolate J-5. This is the first report providing an insight into untapped endophytic actinomycete milieu of Syzygium cumini yet to be explored which might be a promising source for novel antimicrobial agents.

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Section: Resultsmentioning

confidence: 99%

Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens

Saini

Gangwar

Kalia

et al. 2016

JANS

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“…The alkali-insolublt: fraction consists of a glucan/chitin complex which seems to be present in almost all fungal species and probably plays a dominant role in fungal morphogenesis (Sietsma & Wessels, 1991). Following treatment with nitrous acid, most of the glucan that was initially alkali-insoluble became soluble in KOH.…”

Section: Discussionmentioning

confidence: 99%

“…In the yeast species Saccharomyes cerevisiae and Schixosaccbaromyces pombe (Mol & Wessels, 1987 ;Sietsma & Wessels, 1991), and also in some basidiomycetes, e.g. Scbi~oph_Yllm commwe and Agarictls bisporw, the involvement of glucosamine was inferred (Wessels et al, 1989).…”

Section: H S I E T S M Amentioning

confidence: 99%

Chemical and immunological analysis of the Aspergillus fumigatus cell wall

Hearn¹,

Sietsma

1994

Microbiology

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Hyphal-wall preparations of Aspergi//us fumigatus have been analysed by sequential treatment with KOH, nitrous acid and again with KOH. By acidification of the alkali-soluble extract, a polyglucose was precipitated which * Department of Plant Biology, University of Groningen, The Netherlands showed an X-ray diffraction pattern similar to that of (1 -+ 3)-a-glucan. The remainder of the alkali-soluble fraction was precipitated with ethanol; it contained all the mannose, galactose and protein of the wall and, in addition, 6 2 YO of the amino sugars. This wall-associated glycoprotein, following SDS-PAGE and immunoblotting, reacted with antisera raised against several mycelial extracts of A. fumigatus. Sera from patients with aspergilloma have antibodies which recognize components of this glycoprotein. The glycoprotein nature of these antigens was shown by their ability to bind Lens culinaris lectin. In addition, the antigedantibody binding could be disrupted by exposure of antigen to periodate oxidation, hydrolysis with dilute acid or pretreatment with a large excess of an exo-/?-D-galactofuranosidase. The alkaliinsoluble fraction consisted of a covalently linked glucan-chitin complex. Nitrous acid treatment, which specifically disrupts glycosidic linkages involving glucosamine, did not solubilize much material but changed the X-ray diffraction pattern from diffuse to a pattern showing the characteristic lines of crystalline (1 + 3)-g=glucan and chitin. Most of the glucan became alkali-soluble after this treatment, and the insoluble residue appeared to contain crystalline chitin.

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“…Chemical analyses of hyphal walls in S. commune have shown that the major wall components are ␤-glucan and chitin. In addition, the walls contain ␣-glucan and proteins (Sietsma and Wessels, 1991). Among the latter, small hydrophobic proteins, hydrophobins, have been recently shown to be essential for the formation of aerial hyphae (Wessels, 1996).…”

Section: Septal Dissolutionmentioning

confidence: 99%

The Relationship between B-Mating-Type Genes and Nuclear Migration inSchizophyllum commune

Raudaskoski

1998

Fungal Genetics and Biology

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Cell Wall Assembly in Fungal Morphogenesis

Cited by 9 publications

References 57 publications

Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens

Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens

Chemical and immunological analysis of the Aspergillus fumigatus cell wall

The Relationship between B-Mating-Type Genes and Nuclear Migration inSchizophyllum commune

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