Cell wall-bound phenolics from norway spruce (picea abies) needles

Strack, Dieter; Heilemann, Jürgen; Klinkott, Eva-Susan; Wray, Victor

doi:10.1515/znc-1988-1-210

Cited by 28 publications

(13 citation statements)

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“…We discussed in our earlier work (Strack et al 1988a) a possible incorporation of transiently accumulating soluble astragalin into lignin-carbohydrate fractions of the cell walls of Norway spruce needles. However, degradative routes or interconversions in a biosynthetic sequence and conjugation reactions where other compounds are attached to the compound in question (Barz et al 1985) might be involved in this astragalin metabolism.…”

Section: Discussionmentioning

confidence: 99%

Incorporation of kaempferol 3-O-glucoside into the cell walls of Norway spruce needles

Heilemann

Strack

1990

Planta

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(14)C-Labelled CO2 fed to young Norway spruce (Picea abies [L.] Karst.) twigs was rapidly incorporated into kaempferol 3-O-glucoside (astragalin) of the needles. The patterns of the time course of total (per needle weight) and specific radioactivity (per amount of compound) of soluble and insoluble (cell wall-bound) astragalin indicate its transport from a soluble pool within the protoplast to an extraprotoplastic cell wall-bound pool within the needle. This conclusion is supported by measurements of the distribution of radioactivity between the aglycone (kaempferol) and the sugar part (glucose) of the molecule after various chase periods as well as by control experiments to determine the localization of [(14)C]astragalin in the cell wall preparations.

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Section: Discussionmentioning

confidence: 99%

Incorporation of kaempferol 3-O-glucoside into the cell walls of Norway spruce needles

Heilemann

Strack

1990

Planta

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“…Pellets from the soluble metabolite extraction were resuspended in water and transferred to 1-5 ml screw-capped Eppendorf tubes. After centrifugation the supernatants were discarded, and saponifiable wall-bound metabolites extracted by alkaline hydrolysis, using a modification of the method of Strack, Heilemann & Klinkott (1988): pellets were washed twice with 1 ml of water, abs. EtOH, tert-huty\ methyl ether, and dried overnight at room temperature.…”

Section: Analysis Of Cell Wall-bound Phenolics and Ligninmentioning

confidence: 99%

Ozone effects on root‐disease susceptibility and defence responses in mycorrhizal and non‐mycorrhizal seedlings of Scots pine (Pinus sylvestris L.)

Bonello¹,

Heller²,

Sandermann³

1993

New Phytologist

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SUMMARYInteractions between ozone and biotic stress caused by a pathogen, Heterobasidion annosum (Fr.) Bref., in mycorrhizal [Hegeloma crustuliniforme (Bull, ex St. Am.) Quel.] and non-mycorrhizal Scots pine seedlings were investigated using a semi-axenic model system.Ozone exposure (200 nl T^ 8 h d~' for 28 d) increased disease incidence significantly, but mycorrhizal infection completely prevented this negative effect. The presence of the pathogen on the root systems was necessary for the induction of changes in the soluble and wall-bound secondary compounds of roots and needles; ozone alone did not induce such changes. Mycorrhizal infection appeared to have a dampening effect on the induction of these compounds.H. annosum induced a significant accumulation of the two pine stilbenes both locally and systemically in the more susceptible seedlings. In these seedlings ozone had a significant positive effect on the accumulation of both stilbenes in the roots, but it reduced pinosylvin and had no effect on pinosylvin 3-methyl ether in the needles. The catechin content of the roots decreased in the same infected seedlings, but to a larger degree upon ozone treatment. One compound of as yet unknown structure accumulated gradually in the infected roots over the experimental period, and could thus be associated with resistance. Its accumulation was little affected by ozone treatment. Among the root cell wall-bound phenolics analyzed, only lignin-like material showed significant changes. The presence of the pathogen was again necessary for induction, but ozone had an inhibitory effect on this response.Pure pinosylvin applied through the hypocotyls of excised seedlings was shown to be phytotoxic, with the needles displaying discoloration and wilting as observed after pathogenic inoculation, and being characterized by a lower chlorophyll content and increased transpiration. Accumulation of pinosylvin in the needles was detected at amounts comparable to those found in the main experiment.

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“…Ferulic acid has been identified as being ester linked to arabinoxylans in monocotyledonous plants (Kato and Nevins, 1985;Hartley and Ford, 1989;Hartley et al, 1990b) and to pectic arabinans and galactans in dicotyledonous plants (Fry, 1982). Ferulic and p-coumaric acids have been identified as major hydroxycinnamic components bound to gymnosperm secondary cell walls (Strack et al, 1987).…”

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Changes in Dehydrodiferulic Acids and Peroxidase Activity against Ferulic Acid Associated with Cell Walls during Growth of Pinus pinaster Hypocotyl

et al. 1996

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Hydroxycinnamic acids associated with hypocotyl cell walls of dark-grown seedlings of Pinus pinaster Aiton were extracted with 1 N NaOH and identified by gas chromatography-mass spectrometry. l h e main hydroxycinnamic acid found was ferulic acid. Diferulic acid dehydrodimers were also found, with the 8,8-coupled isomer (compound 11) being the dehydrodiferulate present in the highest amount. However, the 5,5-coupled isomer, commonly referred to as diferulic acid, was not detected. Two truxillic acids, 4-4'-dihydroxy-3-3'-dimethoxy-a-truxillic acids I and 11, were tentatively identified. l h e 8,8-coupled dehydrodiferulic acid (compound 11) was the phenolic acid that showed the most conspicuous changes with hypocotyl age as well as along the hypocotyl axis. Peroxidase activity against ferulic acid was found in the apoplastic fluid as well as being ionically and covalently bound to the cell walls. The peroxidase activity increased with hypocotyl age as well as from the subapical toward the basal region of the hypocotyls. A key role in the cell-wall stiffening of 8,8 but not 5,s dimerization of ferulic acid catalyzed by cell-wall peroxidases is proposed.

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Cell wall-bound phenolics from norway spruce (picea abies) needles

Cited by 28 publications

References 0 publications

Incorporation of kaempferol 3-O-glucoside into the cell walls of Norway spruce needles

Incorporation of kaempferol 3-O-glucoside into the cell walls of Norway spruce needles

Ozone effects on root‐disease susceptibility and defence responses in mycorrhizal and non‐mycorrhizal seedlings of Scots pine (Pinus sylvestris L.)

Changes in Dehydrodiferulic Acids and Peroxidase Activity against Ferulic Acid Associated with Cell Walls during Growth of Pinus pinaster Hypocotyl

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