Cell wall growth during the cell cycle ofSchizosaccharomyces pombe

Streiblová, Eva; Wolf, Amnon

doi:10.1002/jobm.3630120808

Cited by 39 publications

(10 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine whether actin normally found at the old ends of cells before NETO and TBZ addition was returning faithfully to old ends during recovery to the cell cortex, we costained rhodamine-phalloidin–stained cdc10-129 cells with Calcofluor. Birth scars created by septum formation stain poorly with Calcofluor, and because new ends initiate growth later than old ends, the new end of a cell can be identified as the end closest to a birth scar ( Streiblova and Wolf, 1972 ; Mitchison and Nurse, 1985 ). Interestingly, in cells containing actin at the branch and at one additional end, this was found to be either the old end or new end in roughly equal measure (28/52 cells vs 24/52 cells, respectively; Fig.…”

Section: Resultsmentioning

confidence: 99%

Regulation of Cell Polarity by Microtubules in Fission Yeast

Sawin

Nurse

1998

The Journal of Cell Biology

138

153

View full text Add to dashboard Cite

To investigate the role of microtubules in regulating cell polarity in Schizosaccharomyces pombe, we have developed a system in which normally cylindrical fission yeast synchronously form branched cells at high frequency upon treatment with the microtubule-depolymerizing drug thiabendazole (TBZ). Branching depends on both elevated temperature and cell cycle state and occurs at high frequency only when TBZ is added to cells that have not yet passed through New-End Take-Off (NETO), the normal transition from monopolar to bipolar growth. This suggests that microtubules may be of greatest physiological importance for the maintenance of cell shape at specific points in the cell cycle. The localization of three different proteins normally found at cell ends—cortical F-actin, tea1, and an ral3 (scd2)–green fluorescent protein (GFP) fusion—is disrupted by TBZ treatment. However, these proteins can eventually return to cell ends in the absence of microtubules, indicating that although their localization to ends normally depends on microtubules, they may recover by alternative mechanisms. In addition, TBZ induces a shift in ral3–GFP distribution from cell ends to the cell middle, suggesting that a protein complex containing ral3 may be part of the cue that specifies the position of branch formation.

show abstract

Section: Resultsmentioning

confidence: 99%

Regulation of Cell Polarity by Microtubules in Fission Yeast

Sawin

Nurse

1998

The Journal of Cell Biology

138

153

View full text Add to dashboard Cite

show abstract

“…Fungal hyphae grow at the apex (12,96,117), and it is not difficult to visualize how this mode of growth gives rise to a tubular shape such as is also seen in fission yeast (124,169). Budding yeast cells usually have an ellipsoidal form; therefore it appears that in addition to extension of the bud tip, equatorial enlargement is required.…”

Section: Generalmentioning

confidence: 95%

Molecular Aspects of Yeast Morphogenesis

Cabib¹

1975

Annu. Rev. Microbiol.

View full text Add to dashboard Cite

“…The cylindrical rod-shaped cells of the fission yeast Schizosaccharomyces pombe grow in length by tip extension (Streiblova and Wolf, 1972). The detailed pattern of growth depends on cell cycle stage; immediately after septation, daughter cells initiate elongation from the 'old' ends-the ends which existed in the previous cell cycle.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of cell morphogenesis in fission yeast--a role for casein kinase II in the establishment of polarized growth.

Snell¹,

Nurse²

1994

The EMBO Journal

154

142

View full text Add to dashboard Cite

We have initiated a study to identify genes regulating cell morphogenesis in the fission yeast Schizosaccharomyces pombe. Five genes have been identified, orb1‐orb5, whose mutation gives rise to spherical cells, indicative of an inability to polarize growth. Two further genes have been identified, tea1 and ban1, whose mutant alleles have disturbed patterns of tip growth, leading to T‐shaped and curved cells. In fission yeast, sites of cell wall deposition are defined by actin localization, with actin distributions and therefore growth patterns undergoing cell cycle stage‐specific reorganization. Studies of double mutants constructed between orb5‐19 and various cdc mutants blocked before and after cell division show that orb5 is required for the re‐establishment of polar growth following cytokinesis. This indicates that the mutant allele orb5‐19 is defective in the reinitiation of polarized growth, even though actin reorganization to the cell tips occurs normally. orb5 encodes a fission yeast homologue of casein kinase II alpha. We propose that this kinase plays a role in the translation of cell polarity into polarized growth, but not in the establishment of polarity itself.

show abstract

Cell wall growth during the cell cycle ofSchizosaccharomyces pombe

Cited by 39 publications

References 8 publications

Regulation of Cell Polarity by Microtubules in Fission Yeast

Regulation of Cell Polarity by Microtubules in Fission Yeast

Molecular Aspects of Yeast Morphogenesis

Genetic analysis of cell morphogenesis in fission yeast--a role for casein kinase II in the establishment of polarized growth.

Contact Info

Product

Resources

About