Cell wall inhibition in a stable streptococcal l-form

Panos, Charles; Cohen, Murray

doi:10.1016/0304-4165(66)90156-5

Cited by 16 publications

(9 citation statements)

References 15 publications

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“…The absence of cell wall in stable L-forms is documented in the literature (31,35). Our findings are similar to those of King et al (18) who did not find cell wall hexosamines in formamide-insoluble extracts from a stable Lform of Streptococcus faecium.…”

Section: P-supporting

confidence: 92%

Characterization of a Stable L-Form ofBacillus subtilis168

1973

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A stable L-form of Bacillus subtilis 168 (sal-1) has been isolated which grows and divides logarithmically in liquid medium with a generation time of 60 min. This mutant does not synthesize cell wall as evidenced by chemical, biochemical, and morphological analyses. Antibiotics which specifically inhibit cell wall biosynthesis do not affect the growth of the L-form. Significant differences exist between the membrane proteins of the bacillary form and the L-form. The relative profile of membrane proteins varies with the salt concentration of the medium in both the L-form and the bacillary form.

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Section: P-supporting

confidence: 92%

Characterization of a Stable L-Form ofBacillus subtilis168

1973

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“…Our observations are consistneric products of incorporation. ent with other demonstrations of membrane defects present in this organism (3,17,22).…”

Section: Synthesis Of the Lysozyme-resistant Polymersupporting

confidence: 78%

“…The acid-extracted product fraction was excluded by Sephadex G-25 and contained muramic acid, glucosamine, and rhamnose. Membrane frag-ments prepared from a stabilized b-form of this strain were shown to catalyze a significantly lower rate of rhamnose incorporation compared with streptococcal membranes (17).…”

mentioning

confidence: 98%

“…Cytidine 5'-diphospho (CDP)[14C]glycerol, [14C]glycerol phosphate (specific activities = 13 mCi/mmol) and unlabeled CDPglycerol were prepared enzymatically (21). Unlabeled TDPrhamnose and TDP[14C]rhamnose (specific activity = 261 mCi/ mmol) (Rf = 0.43) were resolved from glucose-iphosphate substrate (R, = 0.25) by paper chromatography in solvent system II, after enzymatic synthesis (17). After elution from the paper with distilled water, the nucleotide was desalted by gel filtration (Sephadex G-15).…”

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confidence: 99%

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Synthesis of "group polysaccharide" by membranes from Streptococcus pyogenes and its stabilized L-form

Reusch¹,

Panos²

1977

J Bacteriol

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Rhamnose and N-acetylglucosamine (GlcNAc) are incorporated from thymidine 5'-diphosphorhamnose and uridine 5'-diphospho-N-acetylglucosamine (UDPGlcNAc) into membrane fragments prepared from Streptococcus pyogenes but not into membrane fragments prepared from a stabilized L-form of this organism. Incorporation from TDPrhamnose is partially dependent upon UDPGlcNAc and vice versa. The oligomeric GlcNAc and rhamnose-containing products are easily extracted from membrane particles by sedimentation through detergent solutions. They are substantially extracted into methanol but not into chloroform-methanol (2:1). When product containing both radioactive rhamnose and GlcNAc is deacetylated and hydrolyzed briefly in acid, glucosaminyl rhamnose is obtained, but not higher oligomers, suggesting that oligomer synthesis in vitro is terminated because unidentified enzymatic requirements are not satisfied. The data are consistent with the assembly of group A-specific polysaccharide at the cellular membrane with participation of a lipoid anchor (acceptor) molecule.

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“…Panos and Cohen (6,19) have shown that isolated L-form membrane fragments accept only minute amounts of rhamnose from thymidine-5'-diphospho-rhamnose in comparison with membranes isolated from the parental streptococcus, suggesting that a specific lesion exists at the membrane level in the pathway leading to the synthesis of group-specific polysaccharide that is covalently linked to peptidoglycan (11). We now report the results of a comparative investigation of peptidoglycan synthesis by membrane preparations from S. pyogenes and its stabilized b-form which document the existence in the L-form of defects in The metabolism of C,5-isoprenoid alcohol in bacteria.…”

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confidence: 99%

Defective synthesis of lipid intermediates for peptidoglycan formation in a stabilized L-form of Streptococcus pyogenes

Reusch¹,

Panos²

1976

J Bacteriol

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Membrane preparations obtained from a stabilized L-form of Streptococcus pyogenes are incapable of synthesizing peptidoglycan from uridine-5'-diphospho-N-acetyl-muramyl-LAla-i-iso-Glu-L-bys-D-Ala-D-Ala and uridine-5'-diphospho-N-acetyl-D-glucosamine, in contrast with similar preparations from the parental streptococcus. Furthermore, 50-fold higher levels of lipid intermediates which serve as membrane-bound substrates for peptidoglycan synthesis are synthesized in reaction mixtures containing streptococcal membranes than with similar preparations from the L-form. These observations suggest that the inability of this stabilized L-form to form a cell wall in vivo lies, at least in part, in its failure to synthesize significant quantities of the lipid substrates for peptidoglycan synthesis.The pathway by which peptidoglycan is synthesized from uridine-5'-diphospho-N-acetyl-Dmuramyl-L-Ala-D-iso -Glu-L-Lys-D-Ala-D-Ala (UMPPMp) and uridine-5'-diphospho-N-acetyl-D-glucosamine (UDP-GlcNAc) is established. These reactions are catalyzed by membranebound enzymes. In the first step (Fig. 1), phospho-N-acetyl-muramyl-peptapeptide, from UMPPMp, is transferred reversibly from uridine-5'-monophosphate (UMP) to endogenous undecaprenol phosphate (C55OP) by phospho-N-acetyl-i>muramyl-pentapeptide translocase (12). In the second step, incorporation ofN-acetyli-glucosamine from UDP-GlcNAc into undecaprenyl pyrophosphoryl-N-acetyl-D-muramyl-L-300 on August 1, 2020 by guest

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Cell wall inhibition in a stable streptococcal l-form

Cited by 16 publications

References 15 publications

Characterization of a Stable L-Form ofBacillus subtilis168

Characterization of a Stable L-Form ofBacillus subtilis168

Synthesis of "group polysaccharide" by membranes from Streptococcus pyogenes and its stabilized L-form

Defective synthesis of lipid intermediates for peptidoglycan formation in a stabilized L-form of Streptococcus pyogenes

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