Cell-Wall Lipopolysaccharide of the 'Shigella-Like' Escherichia coli 0124. Structure of the Polysaccharide Chain

Abstract

From Escherichiu coli 0124 two lipopolysaccharide preparations were obtained with phenol/water extraction and cetavlon precipitation. Polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate and chemical analysis showed that the two preparations differed only in the extent of the 0-specific polysaccharide moiety. In passive haemagglutination and gel precipitation the two lipopolysaccharide preparations from E. coli 0124 and the corresponding preparations from Shigellu dysenteriur type 3 reacted alike.The 0-specific polysaccharide moiety was characterized with proton magnetic resonance spectroscopy, optical rotation and paper electrophoresis. The constituents were determined by gas chromatography and ion-exchange chromatography. The polysaccharide contained glucose (Glc), galactose (Gal), galactosamine (GalN) and 4-0-( 1 '-carboxyethy1)-D-glucopyranose (glucolactilic acid, GlcLA) in the molar ratios of 1 : 2: 1 : 1. Glucolactilic acid, which has a structure similar to muramic acid, was first found in Sh. dysenteriue. The polysaccharide from E. coli 0124 and oligosaccharides obtained from it by partial acid hydrolysis were subjected to methylation analysis using the method of combined gas chromatography-mass spectrometry. The results indicated that the pentasaccharide repeating unit of the polysaccharide is GlcLA 1-14, Glc

Structural studies of the O‐antigen polysaccharide from the enteroinvasive Escherichia coli O164 cross‐reacting with Shigella dysenteriae type 3

“…The antiserum reacts equally well with both LPS confirming the similarity between the O‐antigens of the E. coli O164 and S. dysenteriae type 3. The structure of the O‐antigen polysaccharide from S. dysenteriae type 3 is further identical to that of E. coli O124 [3].…”

“…Several of the serotypes represented in the EIEC group are either identical or cross‐reactive with some Shigella serotypes [2]. For example, the O‐antigens of E. coli O124 and Shigella dysenteriae type 3 are identical [3]. In addition, the O‐antigens of E. coli O143 and Shigella boydii type 8 have been shown to be identical [4,5].…”

Serology, chemistry, and genetics of O and K antigens of Escherichia coli.

“…strains with an antigenic pattern (combination of polysaccharide antigens) shown in column 2 closely resemble Klebsiella, and those with an antigenic pattern shown in column 5 resemble certain Shigella strains (S. dysenteriae and S. boydii). In fact, identity of surface antigens and serological specificity was found between some E. coli and Shigella strains (27,28,141,275) as well as between some E. coli and Klebsiella strains (21,153,222,230).…”

Isolation and Characterization of Structural Components of Bacillus cereus AHU 1356 Cell Walls

“…4). From the position of these signals, the anomeric configuration of the glycosidic linkages may tentatively be assigned [20,26]. Thus, four residues seem to be linked fi-glycosidically (6 = 4.24,4.34,4.54 and 4.74 ppm) and three residues ct-glycosidically (6 = 5.12, 5.32 and 5.54 ppm).…”

“…Proton magnetic resonance spectra were measured in a JNM PS-100 (Jeol) instrument (100 MHz) in 2 H 2 0 at 90 "C [20] using samples (40 mg) dissolved in 0.5 ml of 'Hz0 and tetramethylsilane as an external standard. ~7 1 .…”