2015
DOI: 10.1021/acsnano.5b03203
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Cellphone-Based Hand-Held Microplate Reader for Point-of-Care Testing of Enzyme-Linked Immunosorbent Assays

Abstract: Standard microplate based enzyme-linked immunosorbent assays (ELISA) are widely utilized for various nanomedicine, molecular sensing, and disease screening applications, and this multiwell plate batched analysis dramatically reduces diagnosis costs per patient compared to nonbatched or nonstandard tests. However, their use in resource-limited and field-settings is inhibited by the necessity for relatively large and expensive readout instruments. To mitigate this problem, we created a hand-held and cost-effecti… Show more

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Cited by 320 publications
(227 citation statements)
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“…Various commercial groups have successfully compacted benchtop fluorescence systems into portable and easy-to-use hand-held devices. Cutting edge advancements in affordable optical sensing and imaging have also been achieved, most notably with the development of cellphone-based designs [58,93] that can have major healthcare impact in low resources settings including the developing world.…”
Section: Optical Analyses For Facilitating Poc Technologiesmentioning
confidence: 99%
“…Various commercial groups have successfully compacted benchtop fluorescence systems into portable and easy-to-use hand-held devices. Cutting edge advancements in affordable optical sensing and imaging have also been achieved, most notably with the development of cellphone-based designs [58,93] that can have major healthcare impact in low resources settings including the developing world.…”
Section: Optical Analyses For Facilitating Poc Technologiesmentioning
confidence: 99%
“…In fact, mobile phone-based imaging and sensing platforms have already been used in a variety of applications, including clinical chemistry, biomedical and environmental monitoring [3][4][5], food analysis [6][7][8], detection of different types of chemical and biological analytes such as cells, parasites [9], bacteria [10], eggs [11], proteins [12], various biomarkers [6,7], nanoparticles [13] and even nucleic acids [14][15][16]. In many of these platforms, processing of the acquired data and the resulting computational analysis are done either on the smartphone or over a local or remote server using a customdeveloped smartphone application.…”
Section: Mobile Phone-enabled Measurement Tools For Research and Clinicmentioning
confidence: 99%
“…Smartphones have also been used as colorimetric readers [3,7,22], for example, to test antimicrobial drug susceptibility [23] and analyze ELISA-based tests, such as mumps immunoglobulin, measles IgG and herpes simplex virus IgG tests, with a very similar level of performance compared with US FDA-approved clinical well-plate readers [3]. This mobile phone-based 96-well reader design [3,7] is composed of a 3D printed opto-mechanical attachment unit that houses 96 multi-mode plastic fiber-optic cables that are each coupled to individual wells of a disposable well plate, enabling the measurement of the transmitted light from the wells, all in parallel and without the need for any mechanical scanning.…”
Section: Editorial Koydemir and Ozcan Future Science Groupmentioning
confidence: 99%
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“…ELISA is a common biochemical assay to generate colorimetric or fluorescent signals using microfluidic chips that leverage the sandwich structure of immobilized capturing antibody, targeting antigen / biomarker, and enzyme-conjugated secondary antibodies. In some well-known studies, a special cellphone attachment was developed to image a 96-well plate with constant sensitivity (Figure 15) [86]. Another advantage of cellphone assays is that they can control microfluidic performance by adjusting electrodes in microfluidic pumps using voltage input control from the cellphone.…”
Section: Sensing Of Proteins and Other Biomarkersmentioning
confidence: 99%