Cells and Extracellular Matrices of Dentin and Pulp: A Biological Basis for Repair and Tissue Engineering

Goldberg, Michel; Smith, Anthony J.; Nagai, Noriyuki

doi:10.2485/jhtb.13.55

Cited by 259 publications

(410 citation statements)

References 41 publications

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“…The biological events underlying the formation of both types of tertiary dentin are distinct, [19][20][21][22] although in both situations the end result is the secretion of the dentin matrix, yet the mechanism by which this happens is different. In the case of reparative dentinogenesis, a cascade of events involving cell division, chemotaxis, cell migration and cytodifferentiation must occur before secretion of matrix can take place.…”

Section: Introductionmentioning

confidence: 99%

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

et al. 2005

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Odontoblasts are postmitotic cells that differentiate from the dental papilla. These cells are responsible for producing the calcified dentin matrix. The pulp-odontoblast interphase contains undifferentiated mesenchymal stem cells, which have the ability to cytodifferentiate into odontoblast-like cells in response to specific signaling molecules. Dentin matrix protein 1 (DMP1) is one of the dentin noncollagenous extracellular matrix proteins that has been implicated in regulation of mineralization. In this study, we have examined the potential role of DMP1 in inducing cytodifferentiation of dental pulp stem cells into odontoblast-like cells and formation of reparative dentin in a rat model. Cavities were drilled and pulps exposed in maxillary first molars. Collagen matrix impregnated with recombinant DMP1 was implanted directly in Group 1, while calcium hydroxide, a commonly used pulp-capping agent was implanted in group 2, collagen matrix that was not impregnated with rDMP1 was implanted directly in group 3, which served as control. Each of these three groups was subdivided into two subgroups, A for 2 weeks time duration and B for 4 weeks duration. At the end of the time period the maxillae were excised, tissues were processed for histological and immunohistochemical evaluations. The results showed that DMP1 could act as a morphogen on undifferentiated mesenchymal cells present in the dentin-pulp complex. These differentiated cells had the potential to regenerate dentin-like tissue, which was confirmed by the presence of collagenous matrix, odontoblast specific markers and calcified deposits. Gene Therapy (2006) 13, 611-620.

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Section: Introductionmentioning

confidence: 99%

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

et al. 2005

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“…Se identificaron como células cilíndricas, distribuidas en forma de empalizada con su núcleo polarizado lejos de la interfase pulpo-dentinal y con presencia de prolongación odontoblástica. Estos hallazgos se encuentran en congruencia con las descripciones de los odontoblastos realizadas por diferentes trabajos publicados (Goldberg & Lasfargues;Goldberg & Smith;Arana-Chavez & Massa;Ruch et al;Yu & Abbott, 2007).…”

Section: Discussionunclassified

“…Este tejido conectivo esta formado por una amplia red de células formadoras de matriz altamente especializadas como fibroblastos pulpares (Goldberg & Smith, 2004) y odontoblastos (AranaChavez & Massa, 2004), células indiferenciadas (Renard et al, 2007) y células inmunocompetentes (Angelova et al, 2004). Estas células establecen relaciones complejas a través de los componentes fibrilar y no fibrilar de la matriz extracelular, presentando una arquitectura celular bien definida en regiones (Goldberg & Smith).…”

Section: Introductionunclassified

Características Histológicas de la Pulpa Dental de Ratones de 4 y 12 Semanas

Salazar

Monsalve

2014

Int. J. Odontostomat.

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RESUMEN:En condiciones fisiológicas, el órgano dentinopulpar no se encuentra asociado a procesos de remodelación y resorción. Los hallazgos histológicos muestran que la pulpa presenta características especiales como tejido conectivo que le confieren su capacidad de adaptación. El propósito del presente estudio fue evaluar las características histológicas de la pulpa dental de ratones de la cepa Albino Suizo de 4 y 12 semanas de edad. Ocho hemimandíbulas de ratones albino suizo de 4 y 12 semanas de edad fueron utilizados. Las hemimandíbulas fueron fijadas en Paraformaldehído al 4%, sometidas a decalcificación con EDTA al 5% durante 10 días a 4ºC e incluidas en Paraplast®. Se obtuvieron secciones de 5 µm que fueron teñidos con H&E, Tricrómico de Masson e Impregnación Argéntica. Las características histológicas de la pulpa revelaron dos zonas principales en ambos grupos de edad: la zona periférica pulpar conformada por un conjunto de células compatibles con odontoblastos y una zona pobre en células, y otra zona central pulpar, rica en fibroblastos y vasos sanguí-neos. Las diferencias entre los grupos de edad se vieron en la expresión de matriz fibrilar de colágeno. Se encontraron semejanzas en la presencia de fibras reticulares entre los grupos. La pulpa dentaria en ratones Albino Suizo mostró una división en dos zonas: central y periférica, con grupos poblacionales bien definidos. La mayor diferencia por edad estuvo en la cantidad de colágeno.

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“…HP can also reach the pulp chamber, where it was shown to reduce cell proliferation, metabolism, and viability. 21 Additionally, it may also reduce the pulp-reparative capacity 22 and induce pulp inflammation, which may be responsible for the TS experienced by the patients during in-office bleaching. 1 All three participants from this study experienced moderate to considerable TS that decreased to a mild level one week after bleaching.…”

Section: Discussionmentioning

confidence: 99%

In-office bleaching effects on the pulp flow and tooth sensitivity – case series

et al. 2015

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Laser Doppler flowmetry (LDF) is a noninvasive method capable of evaluating variations in pulp blood flow (PBF) and pulp vitality. This method has thus far not been used to assess changes in blood flow after in-office bleaching. The aim of this case series report was to measure changes in PBF by LDF in the upper central incisor of three patients submitted to in-office bleaching. The buccal surfaces of the upper arch were bleached with a single session of 35% hydrogen peroxide gel with three 15-min applications. The color was recorded using a value-oriented Vita shade guide before in-office bleaching and one week after the procedure. The tooth sensitivity (TS) in a verbal scale was reported, and PBF was assessed by LDF before, immediately, and one week after the bleaching session. The lower arch was submitted to dental bleaching but not used for data assessment. A whitening degree of 3 to 4 shade guide units was detected. All participants experienced moderate to considerable TS after the procedure. The PBF readings reduced 20% to 40% immediately after bleaching. One week postbleaching, TS and PBF were shown to be equal to baseline values. A reversible decrease of PBF was detected immediately after bleaching, which recovered to the baseline values or showed a slight increase sooner than one week post-bleaching. The LDF method allows detection of pulp blood changes in teeth submitted to in-office bleaching, but further studies are still required.

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Cells and Extracellular Matrices of Dentin and Pulp: A Biological Basis for Repair and Tissue Engineering

Cited by 259 publications

References 41 publications

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

Características Histológicas de la Pulpa Dental de Ratones de 4 y 12 Semanas

In-office bleaching effects on the pulp flow and tooth sensitivity – case series

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