Cellular activity and development of enteric, hepatic and muscle tissues of juvenile pacuPiaractus mesopotamicus(Holmberg 1887) fed with lyophilized bovine colostrum

Abstract

Bovine colostrum, an alternative food of animal origin provided as partial protein source, may constitute an innovative ingredient in fish feeding. Serum insulin-like growth factor 1 (IGF-1) and cellular activity of enteric, hepatic and muscle tissues of juvenile pacu Piaractus mesopotamicus fed with lyophilized bovine colostrum (LBC) were evaluated. Pacu (8.46 AE 0.74 g; 7.75 AE 0.27 cm, n = 18 in triplicate) were stocked into 18 tanks (300 L; 18 fish per tank, in triplicate) and fed with control diet containing 0% of LBC or experimental diet containing 10% and 20% of LBC inclusion for either 30 or 60 days of experiment. IGF-1 serum concentration was not affected (P > 0.05) by the diet containing LBC and experimental period. The intestinal and hepatic cellular activity was not influenced by LBC (P > 0.05); however, intestine protein/RNA decreased at 60 days (P < 0.05). The muscle cellular activity was affected by LBC, highest in fish fed with diet containing 10% of LBC (P < 0.05). Between the periods DNA and protein/RNA increased at 60 days, while RNA, protein/DNA and RNA/DNA ratio decreased in this same period (P < 0.05). Increased DNA muscle content indicates fish growth by hyperplasia.The results of the present study is related with suitable development of enteric, hepatic and muscle tissues and proper condition of juvenile pacu fed with LBC considering the absence of differences in relation to juveniles that consumed diet without colostrum.

Redox balance and tissue development of juvenile Piaractus mesopotamicus subjected to high stocking density and fed dry diets containing nutraceutical food

“…Total protein content, DNA and RNA concentrations are indicators of cellular activity of fish tissues that are under the influence of growth rate and cellular metabolism (Gwak et al, 2003;Chícharo & Chícharo, 2008;Sivaraman et al, 2009). Nordi et al (2017) evaluating the effect of bovine colostrum in the Table 3. Redox balance in different tissues of Piaractus mesopotamicus juveniles juvenile fed with 0, 10, 20 and 30% of lyophilized bovine colostrum and subjected to high stocking density (50 kg fish m -3 ), mean ± standard error.…”