Cellular and developmental properties of fetal hematopoietic stem cells

Leucine-rich Repeat-containing G-protein-coupled Receptor 5 Marks Short-term Hematopoietic Stem and Progenitor Cells during Mouse Embryonic Development

“…A pool of HSCs is formed predominantly in fetal liver on E12 by recruiting definitive HSCs via the blood circulation from extra-hepatic sources (AGM region and/or placenta) (15,16). The definitive HSCs gradually form a massive pool in the fetal liver, becoming the main source of HSCs, which subsequently colonize the bone marrow around the time of birth (17)(18)(19)(20). It is within these limited time windows and in these sites (E11.5-E12.5 AGM region and E11.5-E13.5 fetal liver) that we found Lgr5 expressing cells coincided with the generation and proliferation of HSPCs.…”

“…All procedures were approved by the Institutional Animal Care and Use Committee of SIMR. Hematopoietic Tissues Dissection and Hematopoietic Cells Preparation-The dissection of hematopoietic sites (AGM and fetal liver) and dissociation of single hematopoietic cells were performed as described previously (13,18,21). Slight modifications were done for the latter part.…”

Prion protein is expressed on long-term repopulating hematopoietic stem cells and is important for their self-renewal

“…T he difficulty in purification and study of hematopoietic stem cells (HSCs) is hampered by our limited understanding of the proteins that are specifically expressed on their surface (1)(2)(3). Furthermore, few HSC markers are of functional significance for HSC activity (4).…”

Lis1 is required for the expansion of hematopoietic stem cells in the fetal liver

“…Like many other tissues and organs, the hematopoietic system develops during embryogenesis [2]. One of the challenges of this developing process is to generate a functional HSC pool, which is a prerequisite for seeding bone marrow and thereafter lifelong hematopoiesis [3]. HSC expansion is critical for the establishment of such HSC pool [2].…”