Cellular apoptosis and cardiac dysfunction in STZ‐induced diabetic rats attenuated by anthocyanins via activation of IGFI‐R/PI3K/Akt survival signaling

Abstract

Anthocyanins are known cyto-protective agents against various stress conditions. In this study cardio-protective effect of anthocyanins from black rice against diabetic mellitus (DM) was evaluated using a streptozotocin (STZ)-induced DM rat model. Five-week-old male Wistar rats were administered with STZ (55 mg kg , IP) to induce DM; rats in the treatment group received 250 mg oral anthocyanin/kg/day during the 4-week treatment period. DM and the control rats received normal saline through oral gavage. The results reveal that STZ-induced DM elevates myocardial apoptosis and associated proapoptotic proteins but down-regulates the proteins of IGF1R mediated survival signaling mechanism. Furthermore, the functional parameters such as the ejection-fraction and fraction-shortening in the DM rat hearts declined considerably. However, the rats treated with anthocyanins significantly reduced apoptosis and the associated proapoptotic proteins and further increased the survival signals to restore the cardiac functions in DM rats. Anthocyanin supplementation enhances cardiomyocyte survival and restores cardiac function.

Alpinate Oxyphyllae extracts enhance the longevity and homing of mesenchymal stem cells and augment their protection against senescence in H9c2 cells

“…IGF1 can enhance the activation of Akt through the activation of IGF1R. Various phytochemicals have been reported to effectively modulate the IGF1R/Akt pathway of cell survival in cardiac cells (Asokan Shibu et al, ; C. Y. Huang, Chiang, Pai, & Lin, ; P. C. Huang et al, ; Shibu et al, ). During ROS stress, Sirt1 expression enables resistance to cellular stress by enhancing insulin sensitivity.…”

Short Tetra‐peptide from soy‐protein hydrolysate attenuates hyperglycemia associated damages in H9c2 cells and ICR mice

“…Western blotting was performed as mentioned in previously (Shibu et al, ). H9c2 cells lysed in lysis buffer or the tissue homogenate in tissue lysis buffer (Huang et al, ) were pelleted by centrifugation at 12,000 rpm for 30 min, and the proteins in the supernatant were collected and quantified by Bradford assays (Bio‐Rad, CA, USA). An aliquot containing 30 μg of protein extract was separated by 8% to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and the proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, MA, USA).…”

“…Western blotting was performed as mentioned in previously (Shibu et al, 2018). H9c2 cells lysed in lysis buffer or the tissue homogenate in tissue lysis buffer (Huang et al, 2017) were pelleted by centrifugation at 12,000 rpm for 30 min, and the proteins in the supernatant were collected and quantified by Bradford assays (Bio-Rad, CA, USA).…”

Andrographolide mitigates cardiac apoptosis to provide cardio‐protection in high‐fat‐diet‐induced obese mice

“…In addition, mitochondrial‐dependent intrinsic cardiac apoptosis is also observed in obese condition . Interruption of apoptosis is understood to be one of the possible strategies to reverse or attenuate cardiac disorders and in this regard, several alternative approaches are considered for their anti‐apoptotic benefits against obesity, DM and other pathological conditions . Andrographis paniculata (Burm.…”