Cellular architecture and migration behavior of fibroblast cells on polyhydroxyoctanoate (PHO): A natural polymer of bacterial origin

Abstract

Biodegradable and biocompatible novel materials of natural origin are gaining more and more attention in recent years. These so called biopolymers, characterized by their biointegrity and biocompatibility, find completely new and promising applications in biomedical sciences. The presented work focuses on the medium chain

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

“…The subject of analysis was the actin cytoskeleton. Image analysis, and applied algorithm were performed as described in earlier work [ 21 ]. To quantify differences in cell cytoskeleton on different substrates, the density of actin fibers in each cell region was analyzed.…”

“…Cell tracking was executed using Cell Tracker v 1.0 Software (9000 Rockville Pike, Bethesda, Maryland, USA). The cell tracking protocol, intravital staining of the cell nucleus were performed as described in earlier studies concerning PHO films [ 21 ] using NucBlue (Thermo Fisher Scientific, 168 Third Avenue Waltham, MA USA) reagent based on Hoechst 33342 reagent. Migration analysis experiment lasted for 24 h during which a series of microscopic images were recorded with time resolution of 5 min.…”

“…To increase the statistical significance, for all examined materials separate regions (5–7) of scratch were analyzed on each culturing dish. In parallel, experiments were carried out in reference vessels where the cells were fixed, nucleus and actin fibers were stained at selected time intervals after creating the gap [ 21 ].…”

“…Nevertheless, this material, in combination with other polymers, was shown to be suitable for construction of dressing materials [ 17 , 18 , 19 , 20 ]. PHO Young’s modulus values of 33–41 MPa [ 21 ] indicate that it can mimic the mechanical properties of selected tissues and organs inside humans body [ 22 ]. Material hardness of PHA in the range from 3 to 4.5 MPa suggests that it can find applications where elastomeric properties are desired.…”

Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells

“…Since all these reports bring up other ideas in the final establishment of the processes standing behind the nucleus mechanics, we decided to check the influence of the microenvironment on the presence of the nuclei invaginations and blebs. To explore this further, we stained vimentin, microtubules, actin, and nucleus, and used confocal microscopy imaging for mouse embryonic fibroblast 3T3 cells, cultured on glass, and two substrates of high potential use in medicine: polylactide (PLA) [ 25 , 26 ] and polyhydroxyoctanoate (PHO)—a polymer of biological origin [ 27 , 28 ].…”