Cellular attachment and spatial control of cells using micro-patterned ultra-violet/Ozone treatment in serum enriched media

Mitchell, Stephen; Poulsson, Alexandra H.C.; Davidson, M.R.; Emmison, Neil; Shard, Alexander G.; Bradley, R.H.

doi:10.1016/j.biomaterials.2003.11.010

Cited by 47 publications

(30 citation statements)

References 47 publications

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“…The assignments of surface chemical functionalities were also supported by XPS ( Fig. 1 B and C) and are consistent with previous characterization studies involving UV treatment of virgin polystyrene (26). Consistent with the results from the polymer array (11), robust self-renewal of hESCs was supported by ester/carboxylic acid functionalities yielding oxygen-containing ions in the ToF-SIMS spectra (Fig.…”

supporting

confidence: 90%

Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

Saha

Mei²,

Reisterer³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

135

109

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The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human pluripotent stem cell culture. Chemical and geometrical optimization of the surfaces enables control of early cell aggregation from fully dissociated cells, as predicted from a numerical model of cell migration, and results in significant increases in cell growth of undifferentiated cells. These chemically defined xeno-free substrates generate more than three times the number of cells than feeder-containing substrates per surface area. Further, reprogramming and typical gene-targeting protocols can be readily performed on these engineered surfaces. These substrates provide an attractive cell culture platform for the production of clinically relevant factor-free reprogrammed cells from patient tissue samples and facilitate the definition of standardized scale-up friendly methods for disease modeling and cell therapeutic applications.

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supporting

confidence: 90%

Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

Saha

Mei²,

Reisterer³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

135

109

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“…The rms roughness of the surfaces after treatment decreases slightly from 7 nm to 5.7 nm (scale 50 mm), as shown in Table 3. For comparison, the rms surface roughness of UV/O 3 PS treated surfaces is reported to increase from 3.5 nm to 6.5 nm (scale 20 mm) [4]. It was also reported that the roughness of PS surfaces treated with a CO 2 microwave plasma discharge increases, indicating that degradation of the polymer occurred during the treatment [32].…”

Section: Afmmentioning

confidence: 95%

“…Various surface treatments/modifications have been extensively examined to control the adhesion and growth characteristics of cells on surfaces [1][2][3][4], a matter of prime importance, for instance, for tissue engineering [5]. Understanding the relationship between cells and the physico-chemical properties of surfaces with which they interact (such as wettability, functional groups, topography) is, in fact, essential for the optimisation of cell adhesion, their spreading, and growth thereon [1].…”

Section: Introductionmentioning

confidence: 99%

Biocidal action of ozone-treated polystyrene surfaces on vegetative and sporulated bacteria

Mahfoudh

Barbeau

Moisan

et al. 2010

Applied Surface Science

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“…Oxidation of PEG can be achieved by deep UV (wavelength below 200 nm) exposure through a chromium mask (Azioune et al, 2010;Azioune et al, 2009). Deep UV creates ozone that oxidizes the surface and allows protein adsorption (Mitchell et al, 2004). To create new micropatterned regions in the presence of living cells, we used a Q-switched laser producing 300 picoseconds pulses at 355 nm and a high numerical aperture objective (Fig.…”

Section: Laser Patterningmentioning

confidence: 99%

Reprogramming cell shape with laser nano-patterning

Vignaud¹,

Galland²,

Tseng³

et al. 2012

Journal of Cell Science

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SummaryCell shape in vitro can be directed by geometrically defined micropatterned adhesion substrates. However conventional methods are limited by the fixed micropattern design, which cannot recapitulate the dynamic changes of the cell microenvironment. Here, we manipulate the shape of living cells in real time by using a tightly focused pulsed laser to introduce additional geometrically defined adhesion sites. The sub-micrometer resolution of the laser patterning allowed us to identify the critical distances between cell adhesion sites required for cell shape extension and contraction. This easy-to-handle method allows the precise control of specific actin-based structures that regulate cell architecture. Actin filament bundles or branched meshworks were induced, displaced or removed in response to specific dynamic modifications of the cell adhesion pattern. Isotropic branched actin meshworks could be forced to assemble new stress fibers locally and polarised in response to specific geometrical cues.

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Cellular attachment and spatial control of cells using micro-patterned ultra-violet/Ozone treatment in serum enriched media

Cited by 47 publications

References 47 publications

Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

Biocidal action of ozone-treated polystyrene surfaces on vegetative and sporulated bacteria

Reprogramming cell shape with laser nano-patterning

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