Cellular biomarkers of exposure and biological effect in hepatocytes of turbot (Scophthalmus maximus) exposed to Cd, Cu and Zn and after depuration

Alvarado, N.; Buxens, A.; Mazon, L. I.; Marigómez, Ionan; Soto, Manu

doi:10.1016/j.aquatox.2005.03.024

Cited by 25 publications

(21 citation statements)

References 35 publications

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“…In contrast, the lysosomal system in copper‐exposed organisms tended to display higher surface densities associated with more numerous lysosomes. Our results showed the same pattern than those obtained with Scophthalmus maximus or Mytilus edulis exposed to growing copper concentrations which showed enlarged lysosomes (Alvarado et al, 2005; Dondero et al, 2006). In our study, changes in lysosome structure in copper‐exposed organisms could be due to a fission process of larger ones (Marigómez and Baybay‐Villacorta, 2003), since no variation in the organelle size was observed compared to control.…”

Section: Discussionsupporting

confidence: 87%

Early defense responses in the freshwater bivalve Corbicula fluminea exposed to copper and cadmium: Transcriptional and histochemical studies

Bigot-Clivot¹,

Minguez²,

Giambérini³

et al. 2011

Environmental Toxicology

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The aim of the present study was to measure the early effects of copper (10 and 50 μg L(-1)), cadmium (2, 10, and 50 μg L(-1)) and mixtures of these metals in the freshwater bivalve Corbicula fluminea exposed for 12 h in laboratory. Transcription levels of superoxide dismutase (SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GPx), pi-class glutathione S-transferase (pi-GST), metallothionein (MT) in digestive gland and gills, and response of lysosomal system and neutral lipids in digestive gland were determined after the exposure period. Results showed that lysosomal system, neutral lipids content, and mRNA levels were modified, suggesting their early response against oxidative stress and their important role in cell integrity. The integrated biomarker response was calculated and showed that the effects of the combinations of Cu and Cd on the biomarker responses are additive. MT and pi-GST mRNA expression correspond to the largest ranges of response. As efficient biomarkers should have an early warning capacity, SOD, CAT, Se-GPx, pi-GST, MT transcripts levels, lysosomal system, and neutral lipids could be used as biomarkers of metal contamination in the aquatic environment.

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Section: Discussionsupporting

confidence: 87%

Early defense responses in the freshwater bivalve Corbicula fluminea exposed to copper and cadmium: Transcriptional and histochemical studies

Bigot-Clivot¹,

Minguez²,

Giambérini³

et al. 2011

Environmental Toxicology

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“…According to Alvarado et al (2005), lysosomal metal sequestering is considered a general strategy for reducing metal toxicity. Then, methyl mercury deposits in the cytoplasm of liver and kidney cells of C. carpio corroborate data of others authors (Olsson et al, 1998;Baatrup & Danscher 1987).…”

Section: Discussionmentioning

confidence: 99%

Localization of Inorganic and Organic Mercury in the Liver and Kidney of Cyprinus carpio by Autometallography

Prodocimo

Neto²,

Grötzner³

et al. 2024

Ecotoxicol. Environ. Contam.

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Mercury bioaccumulation in different tissues largely depends upon metal chemical form and has important implications on tissue-specific toxicity. Then, simple tools for mercury tissue localization are very useful for understanding the toxicity of mercurial compounds in fishes. In the present study, inorganic and organic mercury distribution was investigated in carp Cyprinus carpio, utilizing autometallography. Juvenile C. carpio were exposed through force-feeding with mercury contaminated pelleted food at doses of 0.5 µg g -1 of Hg +2 or MeHg twice at 15 days interval, and killed 15 days after the second exposure for sampling. Organic mercury was observed inside hepatocytes and in biliary ducts of liver, whereas inorganic form of mercury was observed only in biliary ducts. In the kidney, mercury was observed mainly in renal tubules, with the predominance of inorganic over organic mercury. The present study demonstrated that autometallography is a useful tool for the tissue localization of two mercurial compounds in the liver and kidney of C. carpio after trophic exposure.Keywords: Autometallography, mercury, liver, kidney, Cyprinus carpio. Localização do mercúrio inorgânico e orgânico em fígado e rim de Cyprinus carpio através da autometalografia ResumoA bioacumulação de mercúrio em diferentes tecidos depende da forma química do metal e tem implicações importantes na toxicidade de tecidos específicos. Deste modo, ferramentas simples para a localização de mercúrio em tecidos são úteis para a compreensão da toxicidade de compostos mercuriais em peixes. No presente estudo, a distribuição de mercúrio inorgânico e orgânico foi investigada em carpa Cyprinus carpio, utilizando autometalografia. Exemplares juvenis de C. carpio foram expostos através de alimentação forçada com pellets de ração contaminada com mercúrio em doses de 0.5 µg g -1 Hg +2 ou MeHg duas vezes em um intervalo de quinze dias, e sacrificados 15 dias após a segunda exposição para amostragem. O mercúrio orgânico foi observado no interior dos hepatócitos e nos ductos biliares. No rim, o mercúrio foi observado principalmente nos túbulos renais, com predominância do mercúrio inorgânico sobre o orgânico. O presente estudo demonstrou que a autometalografia é uma ferramenta útil para a localização tecidual de dois compostos mercuriais em fígado e rim de C. carpio após exposição trófica.Palavras-chave: Autometalografia, mercúrio, fígado, rim, Cyprinus carpio.

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“…It may be mentioned the tripeptide GSH/GSSG by those low molecular weigh proteins, because increase of GSH/GSSG levels in all the tissue has been described as one of the protective mechanisms that fish adopt in the initial phases of exposure to aquatic pollutants (Stephensen et al ., 2002). Cd in living body is bonded to thiol compound, at first moment to GSH/GSSG (Lange et al ., 2002; Belcastro et al ., 2009) and secondly to MT/MTLP (Santovito et al ., 2000; Alvarado et al ., 2005) and increasing of metal content in the body is accompanied enhanced level of thiols. GSH may work as a primary defence (Singhal et al ., 1987).…”

Section: Cadmium – Toxicodynamicsmentioning

confidence: 99%

“…It was found that MT values appeared to be dose dependent and are maintained all throughout the experimental time from other study (Hamilton et al ., 1987). Indeed, depuration produced a significant reduction in MT values (Alvarado et al ., 2005). In the same study was shown that at the beginning of Cd exposure, most of the inherent MT pool was not completely saturated and uncompleted metal stimulated the production of MT mRNA and protein up to the end of the exposure time.…”

Section: Cadmium – Toxicodynamicsmentioning

confidence: 99%

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Can thiol compounds be used as biomarkers of aquatic ecosystem contamination by cadmium?

Kovářová¹,

Svobodová²

2009

Interdisciplinary Toxicology

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Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals we can obviously observe considerable toxicity. It is well known that an organism affected by cadmium (Cd) synthesize low molecular mass thiol compounds rich in cysteine (Cys), such as metallothioneins (MT) and glutathione (GSH/GSSG). The aim of this study was to summarize the effect of Cd on level of thiol compounds in aquatic organisms, and evaluate that the concentrations of thiol compounds are effective indicators of Cd water pollution and explain their potential use in biomonitoring applications.

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Cellular biomarkers of exposure and biological effect in hepatocytes of turbot (Scophthalmus maximus) exposed to Cd, Cu and Zn and after depuration

Cited by 25 publications

References 35 publications

Early defense responses in the freshwater bivalve Corbicula fluminea exposed to copper and cadmium: Transcriptional and histochemical studies

Early defense responses in the freshwater bivalve Corbicula fluminea exposed to copper and cadmium: Transcriptional and histochemical studies

Localization of Inorganic and Organic Mercury in the Liver and Kidney of Cyprinus carpio by Autometallography

Can thiol compounds be used as biomarkers of aquatic ecosystem contamination by cadmium?

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