Cellular composition of the cyclic corpus luteum of the cow

O’Shea, J. D.; Rodgers, R.J.; D’Occhio, M. J.

doi:10.1530/jrf.0.0850483

Cited by 196 publications

(145 citation statements)

References 5 publications

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“…Endothelial cells account for more than half the cells in the bovine CL (O'Shea et al 1989) and have been shown to express a non-genomic progesterone receptor (Falkenstein et al 1996). Thus, much/all of the binding to luteal membranes could be accounted for by contamination with endothelial cell membranes.…”

Section: Membrane Progesterone Receptors In Bovine Follicle Cellsmentioning

confidence: 99%

Bovine ovarian non-genomic progesterone binding sites: presence in follicular and luteal cell membranes

Rae

Menzies²,

Bramley³

1998

Journal of Endocrinology

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We have shown recently that the bovine corpus luteum (CL) possesses specific luteal cell surface membrane binding sites for progesterone. We have now confirmed and extended these observations to compare the subcellular distribution of these binding sites in developing, mature and regressing CL. The median buoyant densities of luteal progesterone binding sites from early-, mid-and late-luteal phase CL were similar (though three of five density profiles for late-luteal phase CL showed association of steroid binding with a fraction with a lower density), and clearly resolved from nuclear, mitochondrial, lysosomal, peroxisomal, Golgi-endoplasmic reticulum-lysosomal and smooth endoplasmic reticulum markers. Specific binding of [3 H]progesterone overlapped with the distributions of 5 -nucleotidase and luteinizing hormone receptor (luteal cell surface membrane markers) in both control and digitonin-treated gradients at all stages of the luteal phase.Since steroidogenic 'large luteal' and 'small luteal' cells of the CL are derived from the granulosa cells (GC) and theca of the preovulatory follicle, we also investigated whether similar receptors were present in the follicle, and describe for the first time specific membrane binding sites for progesterone in purified GC and thecal membranes from healthy bovine follicles of different sizes. Specific binding increased linearly with GC and thecal membrane protein concentration; however, it was detectable only when digitonin was included in the binding incubation. Binding sites were specific for progesterone; unlabelled progesterone competed for [ 3 H]progesterone binding at low concentrations (IC 50 , 35 and 31 nmol/l) compared with testosterone (IC 50 , 905 and 870 nmol/l) and 4 -androstenedione (IC 50 , 1050 and 660 nmol/l) for GC and thecal receptors respectively. In contrast, oestradiol, oestrone, pregnenolone, cortisol, cholesterol, and a genomic progesterone receptor antagonist, RU486, competed poorly.Steroid binding was present in GC and thecal membranes of follicles of all sizes, but [ 3 H]progesterone binding to GC membranes decreased significantly with increasing follicle size (P<0·02), perhaps indicating developmental regulation of GC membrane non-genomic progesterone receptors in the preovulatory bovine follicle. We suggest that these membrane steroid receptors may be involved in the autocrine/paracrine regulation of follicular function by progesterone.

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Section: Membrane Progesterone Receptors In Bovine Follicle Cellsmentioning

confidence: 99%

Bovine ovarian non-genomic progesterone binding sites: presence in follicular and luteal cell membranes

Rae

Menzies²,

Bramley³

1998

Journal of Endocrinology

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“…Expression of class II MHC on macrophages is not surprising and would be expected. However, in midcycle (days 10-12) CL, staining was also observed on cells, whose distribution and architecture relative to the surrounding tissue were reminiscent of endothelial cells (Farin et al 1986, O'Shea et al 1989). In the second experiment, class II MHC was colocalized with a subpopulation of endothelial cells in the midcycle CL.…”

Section: Discussionmentioning

confidence: 99%

The class II major histocompatibility complex molecule BoLA-DR is expressed by endothelial cells of the bovine corpus luteum

2007

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Cells expressing class II major histocompatibility complex (MHC) molecules are found within the corpus luteum (CL) of several species. Expression and localization of class II MHC molecules in the bovine CL were examined in the present study. Immunohistochemical evaluation revealed class II MHC molecules on single cells in early CL (days 4 and 5 post-estrus). Two class II MHC-expressing cell types were observed in midcycle CL (days 10-12 post-estrus), single cells similar to those observed in the early CL, and endothelial cells. Not all endothelial cells expressed class II MHC, and further investigation revealed expression of only one type of class II MHC molecule, DR, on endothelial cells. Class II MHC was also localized to endothelial cells in late CL (day 18 post-estrus). Steroidogenic luteal cells were negative for class II MHC throughout the estrous cycle. Quantitative RT-PCR revealed higher (P!0.05) concentrations of mRNA encoding the a-subunit of DR (DRA) in late CL when compared with those in the early CL. DRA mRNA abundance was also measured in cultures of mixed luteal and luteal endothelial (CLENDO) cells, in the presence or absence of tumor necrosis factor-a (TNF). No differences were found in the DRA mRNA concentration between mixed luteal and CLENDO cell cultures, and TNF had no effect on DRA mRNA concentration in both cell types. Expression of DR by endothelial cells of the midcycle CL may induce anergy of T lymphocytes, or stimulate them to secrete products that enhance normal luteal function.

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“…Bovine CL consists of various cell types including LCs, vascular endothelial cells, smooth muscle cells, fibroblasts, and immune cells (O'Shea et al 1989, Lei et al 1991, Zheng et al 1993, Penny 2000. Therefore, we first examined the mRNA expression of the lymphangiogenic factors in various cells, including LCs and LECs, isolated from the CL, LyECs isolated from the internal iliac lymphatic vessels, and PBMCs and PMNs isolated from peripheral blood (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The development of the bovine CL is accompanied by active angiogenesis, and the structure of blood vessels is retained during pregnancy (O'Shea et al 1989, Lei et al 1991, Beindorff et al 2010. Leukocytes, including monocytes/macrophages, granulocytes, and T lymphocytes, are localized to the CL in several species (Standaert et al 1991, Brannstrom et al 1994, Reibiger & Spanel-Borowski 2000.…”

Section: Introductionmentioning

confidence: 99%

Possible involvement of IFNT in lymphangiogenesis in the corpus luteum during the maternal recognition period in the cow

Nitta¹,

Shirasuna²,

Haneda³

et al. 2011

Reproduction

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The corpus luteum (CL), which secretes large amounts of progesterone and is thus essential for establishing pregnancy, contains various types of immune cells that may play essential roles in CL function by generating immune responses. The lymphatic system is the second circulation system and is necessary for immune function, but the lymphatic system of the bovine CL has not been characterized in detail. We collected bovine CLs on days 12 and 16 of the estrous cycle (C12 and C16) and days 16 and 40 of early pregnancy (P16 and P40). Lymphatic endothelial hyaluronan receptor 1 (LYVE1) protein was detected in the CL by immunohistochemistry and western blotting and increased at P40 compared with C16. The mRNA expression levels of lymphangiogenic factors, such as vascular endothelial growth factor-C (VEGFC), VEGFD, and their common receptor VEGFR3, as well as the lymphatic endothelial cell (LyEC) marker podoplanin, increased in P16 and P40 CLs. Thus, it is suggested that the lymphatic system of the bovine CL reconstitutes during early pregnancy. Interferon tau (IFNT) from the conceptus in the uterus is a candidate for activating luteal lymphangiogenesis during the maternal recognition period (MRP). We found that treatment of LyECs isolated from internal iliac lymphatic vessels with IFNT stimulated LyEC proliferation and significantly increased mRNA expression of VEGFC and IFN-stimulated gene 15. Moreover, both IFNT and VEGFC induced LyECs to form capillary-like tubes in vitro.In conclusion, it is suggested that new lymphangiogenesis in the bovine CL begins during the MRP and that IFNT may mediate this novel phenomenon.

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Cellular composition of the cyclic corpus luteum of the cow

Cited by 196 publications

References 5 publications

Bovine ovarian non-genomic progesterone binding sites: presence in follicular and luteal cell membranes

Bovine ovarian non-genomic progesterone binding sites: presence in follicular and luteal cell membranes

The class II major histocompatibility complex molecule BoLA-DR is expressed by endothelial cells of the bovine corpus luteum

Possible involvement of IFNT in lymphangiogenesis in the corpus luteum during the maternal recognition period in the cow

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