Cellular cooperation in lymphocyte activation

Kimura, Tadashi; Kin, Kohjin; Itoh, Yoshio; Kawai, Tadashi; Morita, Mamoru; Shioiri-Nakano, K

doi:10.1016/0008-8749(80)90064-7

Cited by 26 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast to PHA, the requirement for monocytes in the T-cell proliferative response to SpA is easily and reproducibly documented (8,31). In our experiments, SpA-induced…”

Section: Discussionmentioning

confidence: 65%

Monocytes are required to trigger Ca2+ uptake in the proliferative response of human t lymphocytes to staphylococcus aureus protein A.

Lederman

Lee

Cheung

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

We have used the T-cell mitogen Staphylococcus aureus protein A (SpA) to study the role of monocytes in the early events of T-lymphocyte activation. The mitogenic response of human peripheral blood mononuclear cells (PBM) was compared to the response of populations enriched for T cells by E-rosetting (PBM-E+). In response to SpA, the [3H]thymidine uptake of PBM-E' was reduced by 80% compared to PBM. The reduced response of PBM-E' was completely restored by the addition of irradiated PBM-E-or the monocyte-like human cell line U-937 but not by addition of irradiated PBM-E'. A direct interaction of SpA with monocytes is important since proliferative responses could be generated by preincubation of U-937 with SpA followed by washing and subsequent addition to PBM-E+; incubation of PBM-E+ with SpA followed by washing and subsequent addition of U-937 did not result in a proliferative response. To further delineate the role of the monocyte, we examined the ability of soluble SpA, U-937, or U-937 preincubated with SpA to trigger Ca2+ flux into T lymphocytes, an early step in initiation of the proliferative response. SpA-pretreated U-937, but neither SpA nor monocytes alone, triggered Ca2+ movement into the T lymphocytes. This defines a new role for the monocyte in the early events of T-lymphocyte activation.The monocyte/macrophage is thought to have an important accessory role in T-lymphocyte proliferative responses to antigens and mitogens (1)(2)(3)(4)(5), but the precise mechanisms of monocyte action and their relationship to other early steps in lymphocyte activation are unclear. Proposed monocyte functions include (i) presentation of antigen or mitogen (6), (it) antigen processing (7,8), and (iii) secretion of lymphostimulatory molecules (e.g., interleukin 1) perhaps in concert with antigen presentation (9, 10). It has been difficult to separate clearly these functional properties by using in vitro systems, which usually measure lymphocyte proliferation occumng 72 hr after the initial activating event.Further delineation of the role of monocytes in lymphocyte activation requires identification and measurement of events occurring in the lymphocyte within minutes after activation. Studies of lectin-stimulated mitogenesis have identified such an event: lectin binding triggers a rapid influx of Ca2+ (11)(12)(13)(14)(15), which leads to the formation of an intracellular Ca2+-calmodulin complex (16). The changes in intracellular Ca2' appear to be obligatory early steps in the metabolic cascade leading eventually to DNA synthesis and cell division.In the present study, we have used a monocyte-dependent T-cell mitogen, Staphylococcus aureus protein A (SpA), to examine the participation of monocytes in the induction of Ca2+ movement across T-lymphocyte membranes. Monocytes preincubated with SpA trigger a rapid influx of Ca2+ into lymphocytes, whereas neither SpA nor monocytes alone trigger Ca2+ movement. These studies define a new role for the monocyte in the early events of T-lymphocyte activation. MATERIALS AND ...

show abstract

“…In contrast to PHA, the requirement for monocytes in the T-cell proliferative response to SpA is easily and reproducibly documented (8,31). In our experiments, SpA-induced…”

Section: Discussionmentioning

confidence: 65%

Monocytes are required to trigger Ca2+ uptake in the proliferative response of human t lymphocytes to staphylococcus aureus protein A.

Lederman

Lee

Cheung

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Es wird vermutet daß die erhöhte Menge von ß 2 -Mikroglobulin die unkontrollierte Proliferation des differenzierenden B-Tumorzellklons; der abnorme Plasmazellen und Lymphozyten verschiedener Reifungsstadieh umfaßt (34), reflektiert. Da ß 2 -Mikroglobulin auf Lymphozytenmembranen von Patienten mit chronisch lymphatischer Leukämie, einer B-Zell-Neoplasie, nachgewiesen und von Immunglobulin produzierenden Lymphozytenlinien in das Kulturmedium sezerniert wurde, erscheint diese Annahme als sehr wahrscheinlich (35)(36)(37). Bei 16,6% (22/132) der Patienten mit IgG-Myelom und 40,6% (13/33) der Patienten mit IgA-Myelom wurden erhöhte Serumk'reatinin-und Harnstoffkonzentrationen gleichzeitig mit einer erhöhten Menge von ß 2 -Mikroglobulin beobachtet.…”

Section: Abb 3: Immunelektropherogramm Von Isolierten Igg-paraproteiunclassified

Wertigkeit der quantitativenß₂-Mikroglobulin- und Paraprotein-Bestimmung im Serum von Patienten mit multiplem Myelom und Waldenströms Makroglobulinämie

Fink¹,

Hittmann²,

Schedel³

1984

LaboratoriumsMedizin

View full text Add to dashboard Cite

Serumß 2 -microglobulin andserum paraproteins (SPPR) were determinedin 132patients with IgG myeloma, 33 patients with Ig A myeloma and. 38 patients with Waldenström's macroglobulinaemia (WM) using a competitive enzyme-immunoassay and a fully automated serum electrophoresis System connected on line via RS-232C to a microprocessor-based terminal which displayed the electropherogram. The measured ßi-microglobulin concentrations were significantly (p< 0,0005) increased for patients with IgG-or IgA-myeloma and WM compared to the control group of healthy adults. No correlation was found between serum ß 2 -microglobulin and SPPR for the respective patient groups. For 40 patients with liver cirrhosis and 10patients with chronicpyelonephritis significantly (p < 0.025, p < 0.005) increasedamounts of ß 2 -microglobulin were found respectively. The results suggest, that ß 2 -microglobulin is a useful tool in order to evaluate the kidney manifestation of multiple myeloma or WM. The likewise increased amounts of ß 2 -microglobulin in chronic inflammatory diseases e.g. liver cirrhosis and chronic pyelonephritis limit ß 2 -microglobulin äs an adequate marker for lymphoproliferative disorders. Keywords: ß 2 'microglobulin -paraprotein -antibody deficiency syndrome -multiple myelomamacroglobulinaemia WaldenströmEinleitung ten einwirkt (3, 4). Die von Monozyten vermittelte Suppression der polyklonalen Immunglobulinproduktion Serum ß 2 -Mikroglobulin und Paraproteine dienen bei führt neben anderen Mechanismen zum Antikörperman-Patienten mit multiplem Myelom (MM) und Waidengel. Der humorale Immundefekt zeigt sich klinisch durch Stroms Makroglobulinämie (WM) zur Verlaufskontrolle interkurrente Infektionen der Atemwege und ableitenden (1, 2). Neben den beiden Parametern produzieren die Harnwege (5-7). Bei ca. 50% der Patienten stellen die Tumorzellen einen Piasmozytom-Faktor, der auf Monozyinfektiösen Komplikationen die Todesursache dar (8).

show abstract

Production of specific antibodies against protein A fusion proteins.

Löwenadler¹,

Nilsson²,

Abrahmsén³

et al. 1986

The EMBO Journal

View full text Add to dashboard Cite

The gene for Staphylococcal protein A was fused to the coding sequence of bacterial beta‐galactosidase, alkaline phosphatase and human insulin‐like growth factor I (IGF‐I). The fusion proteins, expressed in bacteria, were purified by affinity chromatography on IgG‐Sepharose and antibodies were raised in rabbits. All three fusion proteins elicited specific antibodies against both the inserted protein sequences and the protein A moiety. In the case of IGF‐I, the protein A moiety in the fusion protein may act as an adjuvant since native IGF‐I alone is a poor immunogen. The results suggest that the protein A fusion system can be used for efficient antibody production against peptides or proteins expressed from cloned or synthetic genes. To facilitate such gene fusions a set of optimized vectors have been constructed.

show abstract

Cellular cooperation in lymphocyte activation

Cited by 26 publications

References 32 publications

Monocytes are required to trigger Ca2+ uptake in the proliferative response of human t lymphocytes to staphylococcus aureus protein A.

Monocytes are required to trigger Ca2+ uptake in the proliferative response of human t lymphocytes to staphylococcus aureus protein A.

Wertigkeit der quantitativenß₂-Mikroglobulin- und Paraprotein-Bestimmung im Serum von Patienten mit multiplem Myelom und Waldenströms Makroglobulinämie

Production of specific antibodies against protein A fusion proteins.

Contact Info

Product

Resources

About

Cellular cooperation in lymphocyte activation

Cited by 26 publications

References 32 publications

Monocytes are required to trigger Ca2+ uptake in the proliferative response of human t lymphocytes to staphylococcus aureus protein A.

Monocytes are required to trigger Ca2+ uptake in the proliferative response of human t lymphocytes to staphylococcus aureus protein A.

Wertigkeit der quantitativenß2-Mikroglobulin- und Paraprotein-Bestimmung im Serum von Patienten mit multiplem Myelom und Waldenströms Makroglobulinämie

Production of specific antibodies against protein A fusion proteins.

Contact Info

Product

Resources

About

Wertigkeit der quantitativenß₂-Mikroglobulin- und Paraprotein-Bestimmung im Serum von Patienten mit multiplem Myelom und Waldenströms Makroglobulinämie