Cellular Dynamics Visualized in Live Cells 
 in Vitro
 and 
 in Vivo
 by Differential Dual-Color Nuclear-Cytoplasmic Fluorescent-Protein Expression

Yamamoto, Norio; Jiang, Ping; Yang, Meng; Xu, Mingxu; Yamauchi, Kensuke; Tsuchiya, Hiroyuki; Tomita, Katsuro; Wahl, Geoffrey M.; Moossa, A. R.; Hoffman, Robert M.

doi:10.1158/0008-5472.can-04-0643

Cited by 132 publications

(129 citation statements)

References 33 publications

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“…HT-1080 dual color Wbrosarcoma cells expressing cytoplasmic DsRed2 and nuclear histone 2B (H2B)-EGFP (Yamamoto et al 2004) were cultured in Dulbecco's modiWed eagle medium (PAN Biotech GmbH, Aidenbach, Germany) supplemented with 10% fetal calf serum (Aurion, Wageningen, The Netherlands), penicillin and streptomycin (both 100 g/ml; PAN) and Hygromycin B (0.2 mg/ml; Invitrogen, Carlsbad, CA, USA) at 37°C in a humiWed 5% CO 2 atmosphere. Dorsal skin-fold chamber model Dorsal skin-fold chambers were transplanted onto 10 to 14-week-old male athymic Balb/c-nu/nu mice (Charles River), as described (Guba et al 2002).…”

Section: Cells and Cell Culturementioning

confidence: 99%

Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model

Alexander

Koehl²,

Hirschberg

et al. 2008

Histochem Cell Biol

225

212

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The metastatic invasion of cancer cells from the primary lesion into the adjacent stroma is a key step in cancer progression, and is associated with poor outcome. The principles of cancer invasion have been experimentally addressed in various in vitro models; however, key steps and mechanisms in vivo remain unclear. Here, we establish a modiWed skin-fold chamber model for orthotopic implantation, growth and invasion of human HT-1080 Wbrosarcoma cells, dynamically reconstructed by epiXuorescence and multiphoton microscopy. This strategy allows repeated imaging of tumor growth, tumor-induced angiogenesis and invasion, as either individual cells, or collective strands and cell masses that move along collagen-rich extracellular matrix and coopt host tissue including striated muscle strands and lymph vessels. This modiWed window model will be suited to address mechanisms of cancer invasion and metastasis, and related experimental therapy.

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Section: Cells and Cell Culturementioning

confidence: 99%

Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model

Alexander

Koehl²,

Hirschberg

et al. 2008

Histochem Cell Biol

225

212

View full text Add to dashboard Cite

show abstract

“…Also, detection and quantification of circulating cells in vivo, in their native state, is potentially important for the early diagnosis of many diseases (e.g., cancer, diabetes, cardiac diseases), or for the study of the influence of the different interventions (e.g., drugs, smoking, radiation) on individual cells [Minamitani et al, 2003;Cherry, 2004]. In particular, to understand the mechanism of the development of metastasis, it is important to monitor tumor cell migration and interaction with other cells [Condeelis and Segall, 2003;Yamamoto et al, 2004;Tozer et al, 2005]. And finally, in vivo study of apoptosis with advanced molecular imaging is crucial for understanding human metabolic and immune system function [Brauer, 2003;Jamin et al, 2003].…”

mentioning

confidence: 99%

In vivo photothermal flow cytometry: Imaging and detection of individual cells in blood and lymph flow

Zharov

Galanzha

Tuchin

2006

J of Cellular Biochemistry

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Flow cytometry is a well-established, powerful technique for studying cells in artificial flow in vitro. This review covers a new potential application of this technique for studying normal and abnormal cells in their native condition in blood or lymph flow in vivo. Specifically, the capabilities of the label-free photothermal (PT) technique for detecting and imaging cells in the microvessel network of rat mesentery are analyzed from the point of view of overcoming the problems of flow cytometry in vivo. These problems include, among others, the influences of light scattering and absorption in vessel walls and surrounding tissues, instability of cell velocity, and cells numbers and positions in a vessel's cross-section. The potential applications of this new approach in cell biochemistry and medicine are discussed, including molecular imaging; studying the metabolism and pathogenesis of many diseases at a cellular level; and monitoring and quantifying metastatic and apoptotic cells, and/or their responses to therapeutic interventions (e.g., drug or radiation), in natural biological environments. J. Cell.

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“…Others have described the use of fluorescent reporter proteins for in vivo cell trafficking and morphogenetic studies in transgenic mice 23 or in mice injected with transfected cells. 24,25 Unlike other fluorescent reporter proteins, the FMB has validated precision for visualizing the phasic progression of the mitotic cell cycle in live cells. 10 The use of high content strategies (days post coitum), CMV-FMB is consistently expressed in the nucleus as shown by co-localization with DAPI stain.…”

Section: Discussionmentioning

confidence: 99%

A Transgenic Mouse Model for High Content, Cell Cycle Phenotype Screening in Live Primary Cells

et al. 2007

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ABBREvIATIOns CMV ABsTRAcTHigh content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds.

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Cellular Dynamics Visualized in Live Cells in Vitro and in Vivo by Differential Dual-Color Nuclear-Cytoplasmic Fluorescent-Protein Expression

Abstract: ABSTRACT

Cited by 132 publications

References 33 publications

Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model

Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model

In vivo photothermal flow cytometry: Imaging and detection of individual cells in blood and lymph flow

A Transgenic Mouse Model for High Content, Cell Cycle Phenotype Screening in Live Primary Cells

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