Cellular expression and genetic control of ABH antigens in primary sensory neurons of marmoset, baboon and man

Mollicone, Rosella; Davies, David R.; Evans, Beryl; Dalix, Anne-Marie; Oriol, Rafaël

doi:10.1016/0165-5728(86)90106-2

Cited by 42 publications

(13 citation statements)

References 22 publications

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“…The presence of H antigen in ganglionic neurons of human teratocarcinomas is in accordance with the presence of H in normal human ganglionic cells [32,37], The absence of H in ganglionic neurons of murine terato carcinomas correlates with its absence from rat and mouse normal ganglionic cells [7], Other nonfucosylated oligosaccharide struc tures have been found on different subsets of normal rat ganglionic cells [38], H antigen is known to be controlled by two structural genes (Se and //) [39]. The presence of H in ganglionic cells is independent of the Se gene [32]; it might be under the control of the H gene but we do not yet have conclusive experimental proof of this genetic control.…”

Section: Discussionmentioning

confidence: 55%

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Localization of H Blood Group Antigen in Ectoblastic Derivatives of Murine Teratocarcinoma

Trojan¹,

Gaillard²,

Vedrenne³

et al. 1987

Tumor Biol

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Ectoblastic derivatives (ectodermal and neuroectodermal components) constitute more than 90% of all structures in the murine teratocarcinoma derived from the PCC4-aza-1 line. This tumor was labeled immunocytochemically with fluoresceinated antibodies to A, B and H blood groups. A and B antigens were always noted to be absent from all structures of the three germ layers. With anti-H, however, embryonic and fetal endodermal components, e.g. alimentary or respiratory duct-like structures, gave positive staining. Mesodermal components, i.e. bone and cartilage structures, fibroblasts and myocytes of mature or embryonal type, were negative. Immature and mature ectodermal components, viz. epidermoid cysts or islets, were always positive. Neuroectodermal components, neuroblastic cysts and differentiated neuronal, glial and ependymal elements were always negative. Ectoblastic and/or neuroectoblastic individual cells or cell clusters, observed in the vicinity of positive differentiating ectodermal and neuroectodermal structures, were positive. Similar cells or clusters were negative when located close to negative neuroectodermal components. The undifferentiated, embryonal carcinoma cells and structures were always negative. These observations are compared to the staining patterns of H antigen in murine embryos and adult mice and in human teratocarcinomas. It is suggested that poorly differentiated, morphologically similar ectoblastic and/or neuroectoblastic structures are positive, if they concern ectodermal components, and negative, if they belong to neuroectodermal components, with the exception of some primary sensory cells that are positive. These last cells are also positive in normal fetal and adult tissues.

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Section: Discussionmentioning

confidence: 55%

“…The neuroectodermal compo nents were negative with the exception of some primary sensory cells or ganglionic neurons ( fig. 8; normal human ganglionic neurons were also H-positive) [32].…”

Section: Immunochemistrymentioning

confidence: 99%

Localization of H Blood Group Antigen in Ectoblastic Derivatives of Murine Teratocarcinoma

Trojan¹,

Gaillard²,

Vedrenne³

et al. 1987

Tumor Biol

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“…Electrophoretic mobility shift assay. A, the labeled probes of the DNA fragments from Ϫ707 to Ϫ626 without (SacB8, lanes 1-4) or with (SacB8m, lanes [5][6][7][8] mutations in the Sp1 binding site were incubated with 0.4 footprinting units of recombinant human Sp1 either alone (lanes 2 and 6) or with the nonlabeled wild-type (lanes 3 and 7) and mutant (lanes 4 and 8) competitors in a 20-fold molar excess. The arrow indicates the binding of recombinant Sp1 to the labeled SacB8 probe, which completely disappeared in the presence of the nonlabeled SacB8 DNA fragment and partially disappeared in the presence of the nonlabeled SacB8m DNA fragment.…”

Section: Fig 5 Effects Of Ngf and Mutations On The Promoter Activitymentioning

confidence: 99%

“…The H antigens are rarely detected in adult nervous tissues of human and other mammals but are present on a subset of neurons. Analyses with Ulex europaeus agglutinin 1 lectin, which binds to type 2 H (Fuc␣1,2Gal␤1,4GlcNAc) determinants, and anti-H antibodies revealed that the expression of H antigens was restricted to olfactory bulb and cochlear hair cells in rats (5,6) and to primary sensory neurons and their axons in human and other primates (7)(8)(9). Most of the H-positive axons of primary sensory neurons were unmyelinated and thought to be C-fibers that mediate nociceptive or thermoceptive inputs or both.…”

mentioning

confidence: 99%

Dorsal Root Ganglia Neuron-specific Promoter Activity of the Rabbit β-Galactoside α1,2-Fucosyltransferase Gene

Hitoshi

Kusunoki

Kanazawa

et al. 1999

Journal of Biological Chemistry

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The rabbit H-blood type ␣1,2-fucosyltransferase (RFT-I), gene and its biosynthetic products, H antigens (Fuc␣1,2Gal␤), are abundantly expressed in a subset of dorsal root ganglia (DRG) neurons. To investigate the regulatory mechanisms for the RFT-I gene expression, we determined the genomic structure and promoter activity of this gene. PCR amplification of the 5 cDNA end analysis revealed two transcriptional start sites, 498 and 82 nucleotides upstream of the translational initiation codon, the latter site yielding a major 3.1-kb transcript specifically expressed in DRG, as revealed by Northern blotting. Promoter analysis of the 5-flanking region of the RFT-I gene using a luciferase gene reporter system demonstrated strong promoter activity in PC12 cells, which express the rat H-type ␣1,2-fucosyltransferase gene, and Neuro2a mouse neuroblastoma cells. Deletion analysis revealed the 704-base pair minimal promoter region flanking the translational initiation codon, for which two distinct promoter activities were detected and differentially used in PC12 and Neuro2a cells. The minimal promoter region contained a GC-rich domain (GC content 80%), in which a Sp1 binding sequence and a GSG-like nerve growth factor-responsive element were found, but lacked TATA-and CAAT-boxes. Promoter analysis with a primary culture of DRG neurons demonstrated that the minimal promoter region of the RFT-I gene was sufficient for the expression of a reporter gene in DRG neurons. We conclude that the TATA-less GC-rich minimal promoter region of the RFT-I gene controls DRG small neuron-specific expression of the RFT-I gene.(for a review, see Ref. 1). The expression of H determinants is strictly regulated temporally and spatially during vertebrate development (2-4). The H antigens are rarely detected in adult nervous tissues of human and other mammals but are present on a subset of neurons. Analyses with Ulex europaeus agglutinin 1 lectin, which binds to type 2 H (Fuc␣1,2Gal␤1,4GlcNAc) determinants, and anti-H antibodies revealed that the expression of H antigens was restricted to olfactory bulb and cochlear hair cells in rats (5, 6) and to primary sensory neurons and their axons in human and other primates (7-9). Most of the H-positive axons of primary sensory neurons were unmyelinated and thought to be C-fibers that mediate nociceptive or thermoceptive inputs or both.We have extended the analysis of the expression of H determinants in the mammalian nervous system using anti-fucosyl GM1 antibodies and U. europaeus agglutinin 1 lectin (10 -13). In the human and rabbit nervous systems, H antigens are abundantly expressed in dorsal root ganglia (DRG), which consist of several types of primary sensory neurons. The antifucosyl GM1 antibodies and U. europaeus agglutinin 1 lectin recognized a subpopulation of neurons in DRG and the dorsal horn of the spinal cord. The anti-fucosyl GM1 antibodies also bound to the satellite cells surrounding the fucosyl GM1-positive neurons (10, 12). In addition, in rabbits, the anti-fucosyl GM1 antibodies bound to...

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“…Importantly, in blood type A individuals, sensory neurons express type A histo-blood group antigens (HBGAs) in Golgi zones (4,12,17,18). Because Ig purification does not remove endogenous mouse antibodies, MAbs may contain murine Ig that reacts with type A HBGAs in tissues from blood type A individuals.…”

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confidence: 99%

Apparent Expression of Varicella-Zoster Virus Proteins in Latency Resulting from Reactivity of Murine and Rabbit Antibodies with Human Blood Group A Determinants in Sensory Neurons

Zerboni

Sobel

Lai

et al. 2012

J Virol

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Analyses of varicella-zoster virus (VZV) protein expression during latency have been discordant, with rare to many positive neurons detected. We show that ascites-derived murine and rabbit antibodies specific for VZV proteins in vitro contain endogenous antibodies that react with human blood type A antigens in neurons. Apparent VZV neuronal staining and blood type A were strongly associated (by a 2 test, ␣ ‫؍‬ 0.0003). Adsorption of ascites-derived monoclonal antibodies or antiserum with type A erythrocytes or the use of in vitro-derived VZV monoclonal antibodies eliminated apparent VZV staining. Animal-derived antibodies must be screened for anti-blood type A reactivity to avoid misidentification of viral proteins in the neurons of the 30 to 40% of individuals who are blood type A. V aricella-zoster virus (VZV) is a human alphaherpesvirus that establishes latency in ganglionic sensory neurons. In autopsied human ganglia, the frequency of individual VZV-positive neurons was 1.0 to 6.9% by single-cell laser capture microdissection (LCMD) and PCR (29). However, immunostaining analyses of VZV protein expression in latently infected neurons have differed significantly. Mahalingam et al. reported infrequent detection of VZV proteins in cadaver ganglia, and in positive tissues VZV protein expression was restricted to very few neurons, a finding confirmed by Kennedy et al. and our recent study (13,16,32). In contrast, Lungu et al. detected VZV proteins in 9 to 24% of neurons in ganglia from three of three subjects (15). Theil et al. found immediate-early 62 (IE62) proteins in ganglia from 38% of individuals and in 3 to 7% of neurons (25). Discrepancies among reports describing the frequencies of VZV protein-positive neurons in autopsied ganglia require further investigation because of their implications for understanding mechanisms of VZV latency.Using a high-potency rabbit anti-IE63 antibody and preimmune serum control, we detected IE63 proteins in ganglia from only 1 of 18 individuals and in Ͻ2.8% of neurons (32). Subsequently, we observed cytoplasmic immunoreactivity in many neurons in ganglia from several individuals when the neurons were stained with monoclonal antibodies (MAbs) to IE62, glycoprotein E (gE), and the capsid protein ORF40 (MAB8616, MAB8612, and MAB8614, respectively; EMD Millipore, Billerica, MA); different lots had similar reactivities. These commercial reagents against IE62 and gE, known to be specific for viral proteins in cultured cells, have been used in studies that report cytoplasmic localization of VZV proteins in neurons (6,9,10,25,26,28). The failure to confirm IE62 and gE detection with other VZV antibodies, the detection of the ORF40 capsid protein detection in latency, and the Golgi zone-like localization of all three VZV proteins suggested a possible staining artifact.The mouse ascites Golgi (MAG) reaction results from endogenous anti-human blood type A antibodies in MAbs derived from mouse ascites (2, 5, 14, 21-23, 30, 31). Importantly, in blood type A individuals, sensory neurons exp...

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Cellular expression and genetic control of ABH antigens in primary sensory neurons of marmoset, baboon and man

Cited by 42 publications

References 22 publications

Localization of H Blood Group Antigen in Ectoblastic Derivatives of Murine Teratocarcinoma

Localization of H Blood Group Antigen in Ectoblastic Derivatives of Murine Teratocarcinoma

Dorsal Root Ganglia Neuron-specific Promoter Activity of the Rabbit β-Galactoside α1,2-Fucosyltransferase Gene

Apparent Expression of Varicella-Zoster Virus Proteins in Latency Resulting from Reactivity of Murine and Rabbit Antibodies with Human Blood Group A Determinants in Sensory Neurons

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