Localization of H Blood Group Antigen in Ectoblastic Derivatives of Murine Teratocarcinoma

Abstract

Ectoblastic derivatives (ectodermal and neuroectodermal components) constitute more than 90% of all structures in the murine teratocarcinoma derived from the PCC4-aza-1 line. This tumor was labeled immunocytochemically with fluoresceinated antibodies to A, B and H blood groups. A and B antigens were always noted to be absent from all structures of the three germ layers. With anti-H, however, embryonic and fetal endodermal components, e.g. alimentary or respiratory duct-like structures, gave positive staining. Mesodermal components, i.e. bone and cartilage structures, fibroblasts and myocytes of mature or embryonal type, were negative. Immature and mature ectodermal components, viz. epidermoid cysts or islets, were always positive. Neuroectodermal components, neuroblastic cysts and differentiated neuronal, glial and ependymal elements were always negative. Ectoblastic and/or neuroectoblastic individual cells or cell clusters, observed in the vicinity of positive differentiating ectodermal and neuroectodermal structures, were positive. Similar cells or clusters were negative when located close to negative neuroectodermal components. The undifferentiated, embryonal carcinoma cells and structures were always negative. These observations are compared to the staining patterns of H antigen in murine embryos and adult mice and in human teratocarcinomas. It is suggested that poorly differentiated, morphologically similar ectoblastic and/or neuroectoblastic structures are positive, if they concern ectodermal components, and negative, if they belong to neuroectodermal components, with the exception of some primary sensory cells that are positive. These last cells are also positive in normal fetal and adult tissues.

Expression of serum albumin and of alphafetoprotein in murine normal and neoplastic primitive embryonic structures

“…The morphology of early stages of neuroectoblastic differentiation in the mouse model of teratocarcinoma has been described (Englehardt et al, 1973;Fox et al, 1983;Martin, 1980;Damyanov and Solter, 1974;Damyanov et al, 1983;Trojan et al, 1984Trojan et al, , 1987. SA transcripts were detected in poorly and moderately differentiated neuroectoblastic structures ( Fig.…”

“…AB: Paraformaldehyde fixation; hematoxylin and eosin staining; x 100, x400, respectively. C: Ethanol fixation; hematoxylin and eosin staining, x 300. pear 6 to 10 days after subcutaneous injection of PCC4 cells into 129 Sv mice (Trojan et al, 1987); a t this time, the teratocarcinomas constitute small tumors (less than 1 cm in diameter). In this work we show that the expression and cellular uptake of SA relative to AFP can serve as well for studies of early stages of developing teratocarcinomas as a tool for differential diagnosis of this tumor.…”

Neoplastic Brain, Glioblastoma, and Immunotherapy

“…To understand the morphology of CNS neoplastic development, the model of mouse teratocarcinoma derived from PCC3 and PCC4 embryonal carcinoma cell lines was investigated. Thanks to this unique model reproducing "caricatural" development of the normal CNS, after examining histologic and electron microscopy sections, the different stages of abnormal nervous tissue histogenesis [1][2][3][4] were established as follows: 1. undifferentiated carcino-embryonic structures; 2. medulloepithelial structures (composed of a mixture of ectoblastic and neuroectoblastic components); 3. neuroblastic structures; and 4. neuroepithelial structures. The final differentiation was the encephaloid tissue.…”