Cellular fatty acids analysis in the identification of lactic acid bacteria

Gilarova, R.; Voldřich, M.; Demnerova, Kateřina; Čeřovský, M.; Dobiáš, J.

doi:10.1016/0168-1605(94)90129-5

Cited by 19 publications

(15 citation statements)

References 4 publications

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“…Gas chromatography of bacterial cellular fatty acid methyl esters is primarily used in clinical microbiology as a means of identifying many medically important gram-negative bacteria such as Pseudomonas (Mukwaya and Welch, 1989) and Campylobacter (Lambert et al, 1987). It has also been applied to Lactobacillus (Rizzo et al, 1987;Gilarova et al, 1994). However, this method was not optimized for the probiotic Lactobacillus species in this study.…”

Section: Resultsmentioning

confidence: 95%

“…Moreover, the testing of many strains resulted in a "no match" result, indicat ing the inadequate nature of the MIDI database for lacto bacilli. Slight variations in cultivation temperature, pH, NaCl, and growth state can profoundly affect the cellular fatty acid contents of lactic acid bacteria (Gilarova et al, 1994). Consistent speciation results are therefore difficult to achieve.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Species-Specific Identification of Commercial Probiotic Strains

Yeung¹,

Sanders²,

Kitts³

et al. 2002

Journal of Dairy Science

118

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Products containing probiotic bacteria are gaining popularity, increasing the importance of their accurate speciation. Unfortunately, studies have suggested that improper labeling of probiotic species is common in com mercial products. Species identification of a bank of com mercial probiotic strains was attempted using partial 16S rDNA sequencing, carbohydrate fermentation anal ysis, and cellular fatty acid methyl ester analysis. Re sults from partial 16S rDNA sequencing indicated dis crepancies between species designations for 26 out of 58 strains tested, including two ATCC Lactobacillus strains. When considering only the commercial strains obtained directly from the manufacturers, 14 of 29 strains carried species designations different from those obtained by partial 16S rDNA sequencing. Strains from six commercial products were species not listed on the label. The discrepancies mainly occurred in Lactobacil lus acidophilus and Lactobacillus casei groups. Carbohy drate fermentation analysis was not sensitive enough to identify species within the L. acidophilus group. Fatty acid methyl ester analysis was found to be variable and inaccurate and is not recommended to identify probi otic lactobacilli.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Species-Specific Identification of Commercial Probiotic Strains

Yeung¹,

Sanders²,

Kitts³

et al. 2002

Journal of Dairy Science

118

View full text Add to dashboard Cite

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“…compositions of the bacteria have been reported to vary depending on their growth stage in purecultured samples (Gilarova et al 1994). However, in intestinal samples containing huge amounts of bacterial cells the possible differences in the growth stages are minimal, without an influence on the GLC analyses.…”

Section: Discussionmentioning

confidence: 99%

Comparison of cellular fatty acid profiles of the microbiota in different gut regions of BALB/c and C57BL/6J mice

Vaahtovuo

Eerola

Toivanen

2005

Antonie Van Leeuwenhoek

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The gastrointestinal tracts of developed animals are colonized by an extremely complex and diverse microbial ecosystem. The host and its microbiota are in close interaction with each other, and the host's genetic characteristics have been suggested to have an influence on the composition of fecal bacteria. However, different sections of gastrointestinal tract harbor microbes typical of each particular section and knowledge of the effect of the host's genotype on the microbiota in the different parts of the gastrointestinal tract is limited. In this study, mice from two inbred strains, C57BL/6J and BALB/c, were raised in identical conditions. Bacterial samples were collected from four parts of the gastrointestinal tract and analyzed for bacterial fatty acids using gas-liquid chromatography (GLC). Significant differences between the microbiota in the feces, in the cecum, in the small bowel and in the stomach were observed. Cecal samples produced more diverse bacterial fatty acid profiles than any of the other samples, revealing a higher bacterial density and a higher number of bacterial species. Further, a significant difference between the two strains of mice was observed throughout the gastrointestinal tract. These findings indicate that the host's genotype has an influence on the gastrointestinal microbiota as a whole, and provide further evidence that the cecum is the most species-rich region of the murine gut.

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“…Table 1 shows strains isolated from coffee pulp silage (obtained from Xalapa, Veracruz, México) and were primarily identified by the MIDI system (Microbial Identification System; Microbial ID, Newark, Delaware, USA), based on analysis of bacterial fatty acid's concentration compared with an internal standard [9].…”

Section: Microorganismsmentioning

confidence: 99%

Microcultures of lactic acid bacteria: characterization and selection of strains, optimization of nutrients and gallic acid concentration

Guzmán-López¹,

Loera²,

Parada³

et al. 2008

J Ind Microbiol Biotechnol

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Eighteen lactic acid bacteria (LAB) strains, isolated from coffee pulp silages were characterized according to both growth and gallic acid (GA) consumption. Prussian blue method was adapted to 96-well microplates to quantify GA in LAB microcultures. Normalized data of growth and GA consumption were used to characterize strains into four phenotypes. A number of 5 LAB strains showed more than 60% of tolerance to GA at 2 g/l; whereas at 10 g/l GA growth inhibition was detected to a different extent depending on each strain, although GA consumption was observed in seven studied strains (>60%). Lactobacillus plantarum L-08 was selected for further studies based on its capacity to degrade GA at 10 g/l (97%). MRS broth and GA concentrations were varied to study the effect on growth of LAB. Cell density and growth rate were optimized by response surface methodology and kinetic analysis. Maximum growth was attained after 7.5 h of cultivation, with a dilution factor of 1-1/2 and a GA concentration between 0.625 and 2.5 g/l. Results indicated that the main factor affecting LAB growth was GA concentration. The main contribution of this study was to propose a novel adaptation of a methodology to characterize and select LAB strains with detoxifying potential of simple phenolics based on GA consumption and tolerance. In addition, the methodology presented in this study integrated the well-known RSM with an experimental design based on successive dilutions.

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Cellular fatty acids analysis in the identification of lactic acid bacteria

Cited by 19 publications

References 4 publications

Species-Specific Identification of Commercial Probiotic Strains

Species-Specific Identification of Commercial Probiotic Strains

Comparison of cellular fatty acid profiles of the microbiota in different gut regions of BALB/c and C57BL/6J mice

Microcultures of lactic acid bacteria: characterization and selection of strains, optimization of nutrients and gallic acid concentration

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