Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up

Kämmerer, Peer W.; Thiem, Daniel G. E.; Alshihri, Abdulmonem; Wittstock, G. H.; Bader, Rainer; Klein, Martine

doi:10.1186/s40729-017-0085-3

Cited by 3 publications

(4 citation statements)

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“… 20 The impact of shear stress on orientation of osteoblast cell clusters, cell morphology, and elongation has been previously reported. 21 To gain a better understanding of how micromotions influence bone cell activity, an in vitro system, which allows application of defined micromotions in a range of 0 µm to 100 µm under static pressure loading conditions, was developed. To approach the activity pattern of patients who underwent total joint arthroplasty, micromotions were applied for six hours a day with a frequency of 1 Hz, which represents a normal gait cycle.…”

Section: Introductionmentioning

confidence: 99%

Effects of interfacial micromotions on vitality and differentiation of human osteoblasts

Ziebart

Fan

Schulze

et al. 2018

Bone & Joint Research

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ObjectivesEnhanced micromotions between the implant and surrounding bone can impair osseointegration, resulting in fibrous encapsulation and aseptic loosening of the implant. Since the effect of micromotions on human bone cells is sparsely investigated, an in vitro system, which allows application of micromotions on bone cells and subsequent investigation of bone cell activity, was developed.MethodsMicromotions ranging from 25 µm to 100 µm were applied as sine or triangle signal with 1 Hz frequency to human osteoblasts seeded on collagen scaffolds. Micromotions were applied for six hours per day over three days. During the micromotions, a static pressure of 527 Pa was exerted on the cells by Ti6Al4V cylinders. Osteoblasts loaded with Ti6Al4V cylinders and unloaded osteoblasts without micromotions served as controls. Subsequently, cell viability, expression of the osteogenic markers collagen type I, alkaline phosphatase, and osteocalcin, as well as gene expression of osteoprotegerin, receptor activator of NF-κB ligand, matrix metalloproteinase-1, and tissue inhibitor of metalloproteinase-1, were investigated.ResultsLive and dead cell numbers were higher after 25 µm sine and 50 µm triangle micromotions compared with loaded controls. Collagen type I synthesis was downregulated in respective samples. The metabolic activity and osteocalcin expression level were higher in samples treated with 25 µm micromotions compared with the loaded controls. Furthermore, static loading and micromotions decreased the osteoprotegerin/receptor activator of NF-κB ligand ratio.ConclusionOur system enables investigation of the behaviour of bone cells at the bone-implant interface under shear stress induced by micromotions. We could demonstrate that micromotions applied under static pressure conditions have a significant impact on the activity of osteoblasts seeded on collagen scaffolds. In future studies, higher mechanical stress will be applied and different implant surface structures will be considered.Cite this article: J. Ziebart, S. Fan, C. Schulze, P. W. Kämmerer, R. Bader, A. Jonitz-Heincke. Effects of interfacial micromotions on vitality and differentiation of human osteoblasts. Bone Joint Res 2018;7:187–195. DOI: 10.1302/2046-3758.72.BJR-2017-0228.R1.

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Section: Introductionmentioning

confidence: 99%

Effects of interfacial micromotions on vitality and differentiation of human osteoblasts

Ziebart

Fan

Schulze

et al. 2018

Bone & Joint Research

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“…The results showed that MSCs responded to low shear stress, and 32 specific proteins were identified, of which 10 were upregulated. The effect of FSS on osteoblasts is reported to be detectable at 10 dyn per cm 2 , 77 which is at the lower end of the physiological range (8–30 dyn per cm 2 ). 32 , 59 , 78 Riehl et al 79 investigated the effect of physiologically relevant shear stresses at 2, 15 and 25 dyn per cm 2 on MSCs and found that flow shear stress levels had a significant influence on MSC migration.…”

Section: Normogravitymentioning

confidence: 99%

“… Xing et al 232 5 dyn per cm 2 Parallel plate Rat Osteoblasts Increased Col-I and proliferation. Decreased ALP, proliferation Kämmerer et al 77 10 dyn per cm 2 , 24 h (shear at the center=1 dyn per cm 2 with 10 dyn per cm 2 at the periphery) Rotating at 200 r·min −1 Osteoblasts Actin filaments re-aligned themselves towards orientation of fluid flow Malone et al 62 12 dyn per cm 2 , 1 Hz, 1 h Oscillatory Murine Osteoblasts PGE2 increased 3-fold ( P < 0.05). No increase in F-actin development Tan et al 73 0.4-1.0 dyn per cm 2 , 5 Hz, 1 h Mean stress of 0.7 Pa Pulsating 0.3 Pa Chicken Osteocytes NO production decreased ( P < 0.05).…”

Section: Normogravitymentioning

confidence: 99%

Changes in interstitial fluid flow, mass transport and the bone cell response in microgravity and normogravity

et al. 2022

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In recent years, our scientific interest in spaceflight has grown exponentially and resulted in a thriving area of research, with hundreds of astronauts spending months of their time in space. A recent shift toward pursuing territories farther afield, aiming at near-Earth asteroids, the Moon, and Mars combined with the anticipated availability of commercial flights to space in the near future, warrants continued understanding of the human physiological processes and response mechanisms when in this extreme environment. Acute skeletal loss, more severe than any bone loss seen on Earth, has significant implications for deep space exploration, and it remains elusive as to why there is such a magnitude of difference between bone loss on Earth and loss in microgravity. The removal of gravity eliminates a critical primary mechano-stimulus, and when combined with exposure to both galactic and solar cosmic radiation, healthy human tissue function can be negatively affected. An additional effect found in microgravity, and one with limited insight, involves changes in dynamic fluid flow. Fluids provide the most fundamental way to transport chemical and biochemical elements within our bodies and apply an essential mechano-stimulus to cells. Furthermore, the cell cytoplasm is not a simple liquid, and fluid transport phenomena together with viscoelastic deformation of the cytoskeleton play key roles in cell function. In microgravity, flow behavior changes drastically, and the impact on cells within the porous system of bone and the influence of an expanding level of adiposity are not well understood. This review explores the role of interstitial fluid motion and solute transport in porous bone under two different conditions: normogravity and microgravity.

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“…1 ESW has been shown to stimulate tissue repair mainly by inducing the collapse of ultrastructural vesicles through negative pressure and causing asymmetrical fluid streams within the tissue. 2,3 Mechanical impact is presumed to exert similar beneficial effects, for example, in human osteoblasts, 4,5 although the underlying mechanism is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Extracorporeal Shock Wave Stimulates Angiogenesis and Collagen Production in Facial Soft Tissue

Alshihri

Kämmerer

Heimes

et al. 2020

Journal of Surgical Research

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Skin Angiogenesis Dermal thicknessFlap surgery a b s t r a c t Background: This study investigated the efficacy of extracorporeal shock wave (ESW) application in stimulating dermal thickness, vascularity, and collagen synthesis of facial skin in a large animal model. Materials and methods: The facial skin of the maxillary and mandibular areas of goats (n ¼ 6 per group) was treated with ESWs of different intensities (0.15 and 0.45 mJ/mm 2 ; 1000 pulses). After 4 d, histology and immunohistochemistry were used to evaluate the following: dermal thickness, total number and abundance of microvessels, amount of type 1 collagen, and a-smooth muscle actin expression. Results: Dermal thickness, number and abundance of microvessels, and collagen synthesis increased after ESW application at both intensities (each P < 0.05). When comparing ESW groups, the highest collagen abundance was seen after 0.15 mJ/mm 2 (P ¼ 0.034), whereas the highest number of microvessels was detected after treatment with 0.45 mJ/mm 2 (P ¼ 0.002).Conclusions: A single-session application of focused low-energy ESWs to facial skin can increase dermal thickness by stimulating collagen production and local microcirculation.These findings commend the technique for future investigation for pretreatment of local or microvascular skin flaps to enhance tissue healing. ª

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Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up

Cited by 3 publications

References 39 publications

Effects of interfacial micromotions on vitality and differentiation of human osteoblasts

Effects of interfacial micromotions on vitality and differentiation of human osteoblasts

Changes in interstitial fluid flow, mass transport and the bone cell response in microgravity and normogravity

Extracorporeal Shock Wave Stimulates Angiogenesis and Collagen Production in Facial Soft Tissue

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