Cellular glucose transport and glucotransporter 4 expression as a therapeutic target: clinical and experimental studies

Czech, Anna; Piątkiewicz, Paweł; Tatoń, J

doi:10.1007/s00005-009-0052-7

Cited by 4 publications

(1 citation statement)

References 31 publications

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“…PBMCs are increasingly used as a surrogate model for glucose disposal. Recent studies have shown that PBMCs have insulin-sensitive GLUT4 activity and that GLUT4 increases on the membrane of monocytes [ 33 , 34 , 35 ] and lymphocytes [ 36 , 37 ] in response to insulin. Furthermore, we previously reported alterations of the insulin signaling cascade in PBMCs from obese individuals [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Dynamic Changes of BVRA Protein Levels Occur in Response to Insulin: A Pilot Study in Humans

Cimini

Tramutola

Barchetta

et al. 2023

IJMS

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Biliverdin reductase-A (BVRA) is involved in the regulation of insulin signaling and the maintenance of glucose homeostasis. Previous research showed that BVRA alterations are associated with the aberrant activation of insulin signaling in dysmetabolic conditions. However, whether BVRA protein levels change dynamically within the cells in response to insulin and/or glucose remains an open question. To this aim, we evaluated changes of intracellular BVRA levels in peripheral blood mononuclear cells (PBMC) collected during the oral glucose tolerance test (OGTT) in a group of subjects with different levels of insulin sensitivity. Furthermore, we looked for significant correlations with clinical measures. Our data show that BVRA levels change dynamically during the OGTT in response to insulin, and greater BVRA variations occur in those subjects with lower insulin sensitivity. Changes of BVRA significantly correlate with indexes of increased insulin resistance and insulin secretion (HOMA-IR, HOMA-β, and insulinogenic index). At the multivariate regression analysis, the insulinogenic index independently predicted increased BVRA area under curve (AUC) during the OGTT. This pilot study showed, for the first time, that intracellular BVRA protein levels change in response to insulin during OGTT and are greater in subjects with lower insulin sensitivity, supporting the role of BVR-A in the dynamic regulation of the insulin signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Dynamic Changes of BVRA Protein Levels Occur in Response to Insulin: A Pilot Study in Humans

Cimini

Tramutola

Barchetta

et al. 2023

IJMS

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The Dysfunction of NK Cells in Patients with Type 2 Diabetes and Colon Cancer

Piątkiewicz

Miłek

Bernat-Karpińska

et al. 2013

Arch. Immunol. Ther. Exp.

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Glucose metabolism disorders influence anticarcinogenic function of natural killer (NK) cells. The aim of this study was to evaluate the number and cytotoxic activity of NK cells in type 2 diabetic (T2D) patients with negative family history of cancer, type 2 diabetic subjects with newly diagnosed untreated colon cancer (T2DCC) and patients without type 2 diabetes with newly diagnosed, untreated colon cancer (CC). Incubation tests were performed in 18 T2D patients, treated with diet and oral antidiabetic agents, 16 T2DCC; cT1-4N0M0 (c-clinical diagnosis based on computed tomography, colonoscopy and histopathology) treated with diet and oral antidiabetic agents and 16 normoglycemic CC; cT1-4N0M0. Control group included 18 metabolically healthy (with normal fasting glucose and normal glucose tolerance) subjects (HS) with negative family history of cancer, matched by age, BMI and waist circumference. Peripheral blood mononuclear cells were isolated by means of gradient centrifugation. The K562 human erythroleukemia cell line served as the standard target for human NK cytotoxicity assay. The T2D revealed an increased number of NK cells (13.56 ± 5.9 vs 9.50 ± 4.8 %; p < 0.05) when compared with HS, yet these cells had a decreased activity (3.3 ± 2.5 vs 9.4 ± 3.6 %; p < 0.01). The CC demonstrated a decreased activity (2.9 ± 1.8 %; p < 0.01) but a similar number (8.82 ± 3.7 %; not significant) of NK cells when compared to HS. The T2DCC NK cells were characterized by trace cytotoxic activity (1.1 ± 0.7 %; p < 0.01) and nearly three times greater amount (21.24 ± 7.5 %; p < 0.01) when compared to T2D. Type 2 diabetes and CC are associated with disadvantageous alterations of NK cells, leading to impairment in their cytotoxic activity. The impaired activity of NK cells in T2D can be involved in the increased carcinogenic risk and can promote a higher incidence of CC.

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Reduced biliverdin reductase-A levels are associated with early alterations of insulin signaling in obesity

Cimini

Arena

Barchetta

et al. 2019

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Cellular glucose transport and glucotransporter 4 expression as a therapeutic target: clinical and experimental studies

Cited by 4 publications

References 31 publications

Dynamic Changes of BVRA Protein Levels Occur in Response to Insulin: A Pilot Study in Humans

Dynamic Changes of BVRA Protein Levels Occur in Response to Insulin: A Pilot Study in Humans

The Dysfunction of NK Cells in Patients with Type 2 Diabetes and Colon Cancer

Reduced biliverdin reductase-A levels are associated with early alterations of insulin signaling in obesity

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