Cellular Lnc_209997 suppresses Bombyx mori nucleopolyhedrovirus replication by targeting <scp>miR</scp>‐275‐5p in B. mori

Su, Lin; Zhang, S. L.; Yin, Haotong; Zhao, Zhi Meng; Chen, Z. K.; Shen, Manman; Zhang, Z. D.; Guo, Xintao; Wu, Ping

doi:10.1111/imb.12760

Cited by 13 publications

(6 citation statements)

References 35 publications

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“…The role of lncRNAs in insect immunity and permissiveness to pathogens has been investigated in other species, particularly D. melanogaster, for which significant findings regarding its immune response against pathogenic viruses and bacteria have been reported [15,43,44]. Research has also been carried out in lepidopteran species such as Bombyx mori and Pectinophora gossypiella, in which specific lncRNAs have been implicated in the response to viral infection [45,46]. Despite the progress made in other species, lncRNA research in mosquito species has until now been limited and their implication in vector competence and immune responses to human pathogens has not been extensively addressed.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNAs regulate Aedes aegypti vector competence for Zika virus and reproduction

et al. 2023

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Long non-coding RNAs (lncRNAs) play critical regulatory roles in various cellular and metabolic processes in mosquitoes and all other organisms studied thus far. In particular, their involvement in essential processes such as reproduction makes them potential targets for the development of novel pest control approaches. However, their function in mosquito biology remains largely unexplored. To elucidate the role of lncRNAs in mosquitoes’ reproduction and vector competence for arboviruses, we have implemented a computational and experimental pipeline to mine, screen, and characterize lncRNAs related to these two biological processes. Through analysis of publicly available Zika virus (ZIKV) infection-regulated Aedes aegypti transcriptomes, at least six lncRNAs were identified as being significantly upregulated in response to infection in various mosquito tissues. The roles of these ZIKV-regulated lncRNAs (designated Zinc1, Zinc2, Zinc3, Zinc9, Zinc10 and Zinc22), were further investigated by dsRNA-mediated silencing studies. Our results show that silencing of Zinc1, Zinc2, and Zinc22 renders mosquitoes significantly less permissive to ZIKV infection, while silencing of Zinc22 also reduces fecundity, indicating a potential role for Zinc22 in trade-offs between vector competence and reproduction. We also found that silencing of Zinc9 significantly increases fecundity but has no effect on ZIKV infection, suggesting that Zinc9 may be a negative regulator of oviposition. Our work demonstrates that some lncRNAs play host factor roles by facilitating viral infection in mosquitoes. We also show that lncRNAs can influence both mosquito reproduction and permissiveness to virus infection, two biological systems with important roles in mosquito vectorial capacity.

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Section: Discussionmentioning

confidence: 99%

Long non-coding RNAs regulate Aedes aegypti vector competence for Zika virus and reproduction

et al. 2023

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“…Furthermore, the results indicated that after infection with BmNPV, the silkworm could regulate its self-immunity through the lncRNA-miRNA-mRNA pathway ( Zhang S. et al, 2020 ). In the fat body, Lnc_209997 was significantly down-regulated in BmNPV-infected silkworms, and Lnc_209997 could also inhibit BmNPV replication by regulating miR-275-5p expression ( Lin et al, 2022 ). Therefore, to promote its replication in vivo , BmNPV reduced the expression of host Lnc_209997 and indirectly regulated miR-275-5p ( Lin et al, 2022 ).…”

Section: Regulation Of Bmnpv Infection In the Silkworm By Ncrnasmentioning

confidence: 99%

“…In the fat body, Lnc_209997 was significantly down-regulated in BmNPV-infected silkworms, and Lnc_209997 could also inhibit BmNPV replication by regulating miR-275-5p expression ( Lin et al, 2022 ). Therefore, to promote its replication in vivo , BmNPV reduced the expression of host Lnc_209997 and indirectly regulated miR-275-5p ( Lin et al, 2022 ). Since these studies, the function of lncRNA is becoming better understood.…”

Section: Regulation Of Bmnpv Infection In the Silkworm By Ncrnasmentioning

confidence: 99%

Multi-omics study and ncRNA regulation of anti-BmNPV in silkworms, Bombyx mori: an update

Fan,

Andoh,

Chen

2023

Front. Microbiol.

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Bombyx mori silkworm is an important economic insect which has a significant contribution to the improvement of the economy. Bombyx mori nucleopolyhedrovirus (BmNPV) is a vitally significant purulent virus that impedes the sustainable and stable development of the silkworm industry, resulting in substantial economic losses. In recent years, with the development of biotechnology, transcriptomics, proteomics, metabolomics, and the related techniques have been used to select BmNPV-resistant genes, proteins, and metabolites. The regulatory networks between viruses and hosts have been gradually clarified with the discovery of ncRNAs, such as miRNA, lncRNA, and circRNA in cells. Thus, this paper aims to highlight the results of current multi-omics and ncRNA studies on BmNPV resistance in the silkworm, providing some references for resistant strategies in the silkworm to BmNPV.

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“…It is well documented that a growing number of insect lncRNAs exert roles in insect immune, however, few in‐depth functional analyses of lncRNA are available in B. mori . Lin et al (2022) found silkworm Lnc_209997 could suppress BmNPV replication by targeting miR‐275‐5p in B. mori , implying lncRNA may play a role in the immune response of silkworm. LncRNA4 showed a similar expression pattern to that of Toll 4 and could be used as a bait to titrate the dimer of Toll, which is required for the production of antimicrobial peptides (Satyavathi et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

LincRNA_XR209691.3 could promote Bombyx mori nucleopolyhedrovirus replication by interacting with BmHSP70

Yin

Zhao

et al. 2022

Insect Molecular Biology

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Long non-coding RNAs (lncRNAs), a class of transcripts exceeding 200 nucleotides and lacking protein coding potential, have been proven to play important roles in viral infection and host immunity. Bombyx mori nucleopolyhedrovirus (BmNPV) is an important pathogen, which causes the silkworm disease and leads to a huge challenge to the sericultural industry. At present, research on the roles of insect lncRNAs in host-virus interaction are relatively few. In this study, we explored the function of lincR-NA_XR209691.3 that was significantly up-regulated in the silkworm fat body upon BmNPV infection. Firstly, the subcellular localization experiment confirmed that lincR-NA_XR209691.3 was present in both the nucleus and cytoplasm. Enhancing the expression of lincRNA_XR209691.3 in BmN cells could promote the proliferation of BmNPV, while inhibition of lincRNA_XR209691.3 by RNA interference suppresses the proliferation of BmNPV. Combining RNA pull-down and mass spectrometry, we identified the host and BmNPV proteins that could interact with lincRNA_XR209691.3. Next, by using truncation experiment and RNA immunoprecipitation (RIP) assay, it was found that lincR-NA_XR209691.3 could bind to the Actin domain of BmHSP70. Subsequently, overexpression of lncRNA_XR209691.3 in BmN cells promoted the expression of BmHSP70, while knockdown of BmHsp70 suppressed the replication of BmNPV. Based on the above results, it is speculated that lincRNA_XR209691.3 could promote the proliferation of BmNPV through interaction with BmHSP70, possibly by improving the stability of BmHSP70 and thereby enhancing the expression of BmHSP70. Our results shed light on the lncRNA function in insect-pathogen interactions and provide a new clue to elucidate the molecular mechanism of BmNPV infection.

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Cellular Lnc_209997 suppresses Bombyx mori nucleopolyhedrovirus replication by targeting miR‐275‐5p in B. mori

Cited by 13 publications

References 35 publications

Long non-coding RNAs regulate Aedes aegypti vector competence for Zika virus and reproduction

Long non-coding RNAs regulate Aedes aegypti vector competence for Zika virus and reproduction

Multi-omics study and ncRNA regulation of anti-BmNPV in silkworms, Bombyx mori: an update

LincRNA_XR209691.3 could promote Bombyx mori nucleopolyhedrovirus replication by interacting with BmHSP70

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