Cellular interactions with synthetic polymer surfaces in culture

Lydon, Myra; Minett, T.W; Tighe, Brian

doi:10.1016/0142-9612(85)90100-0

Cited by 242 publications

(120 citation statements)

References 25 publications

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“…In orthopaedic applications, PMMA-based bone cements are extensively used for the fixation of total joint replacements, as the material supports cell adhesion and spreading (Lydon et al, 1985;van Wachem et al, 1985van Wachem et al, , 1987. In a perioperative contamination model, PMMA showed better tissue cell adhesion and spreading in the presence of adhering S. epidermidis than other commonly used biomaterials (Subbiahdoss et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Mammalian cell growth versus biofilm formation on biomaterial surfaces in an in vitro post-operative contamination model

et al. 2010

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Biomaterial-associated infections are the major cause of implant failure and can develop many years after implantation. Success or failure of an implant depends on the balance between host tissue integration and bacterial colonization. Here, we describe a new in vitro model for the postoperative bacterial contamination of implant surfaces and investigate the effects of contamination on the balance between mammalian cell growth and bacterial biofilm formation. U2OS osteosarcoma cells were seeded on poly(methyl methacrylate) in different densities and allowed to grow for 24 h in a parallel-plate flow chamber at a low shear rate (0.14 s "1 ), followed by contamination with Staphylococcus epidermidis ATCC 35983 at a shear rate of 11 s "1 . The U2OS cells and staphylococci were allowed to grow simultaneously for another 24 h under lowshear conditions (0.14 s "1

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Section: Discussionmentioning

confidence: 99%

Mammalian cell growth versus biofilm formation on biomaterial surfaces in an in vitro post-operative contamination model

et al. 2010

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“…This complexity in the literature is exacerbated by the fact that there are a number of methods of assessing cell-surface interaction that do not fall in the category of cell-attachment kinetics. These include measurement of cell spreading [90][91][92], cell interfacial energy [93], forces required to dislodge adherent cells [94][95][96], and use of detachment indices [97].…”

Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, CRL-11372) to substrata with varying surface chemistry and topography is reviewed within the context of extant cell-adhesion theory. Cell-attachment and proliferation kinetics are compared using morphology as a leading indicator of cell phenotype. Expression of (α 2 , α 3 , α 4 , α 5 , α v , β 1 and β 3 ) integrins, vinculin, as well as secretion of osteopontin and type I collagen supplement this visual assessment of hFOB growth. It is concluded that significant cell-adhesion events -contact, attachment, spreading, and proliferation -are similar on all surfaces, independent of substratum surface chemistry/energy. However, this sequence of events is significantly delayed and attenuated on hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency and long induction periods before cells engage in an exponential-growth phase. Results suggest that a 'time-cell-substratum compatibility-superposition-principle' is at work wherein similar bioadhesive outcomes can be ultimately achieved on all surface types with varying hydrophilicity, but the time required to arrive at this outcome increases with decreasing cellsubstratum compatibility. Genomic and proteomic tools offer unprecedented opportunity to directly measure changes in the cellular machinery that lead to observed cell responses to different materials. But for the purpose of measuring structure-property relationships that can guide biomaterial development, genomic/proteomic tools should be applied early in the adhesion/spreading process before cells have an opportunity to significantly remodel the cell-substratum interface, effectively erasing cause-and-effect relationships between cell cell-substratum compatibility and substratum properties.Impact Statement-This review quantifies relationships among cell phenotype, substratum surface chemistry/energy, topography, and cell-substratum contact time for the model osteoblast cell

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“…While hydrophilic polymers such as poly(hydroxyethyl methacrylate) and poly(hydroxyethyl acrylate, HEA) are not adhesive for either fibroblasts or other cell lines, hydrophobic polymers of the same series such as poly(methyl methacrylate, PMMA), or poly(ethyl methacrylate), did allow cell anchorage (23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

Sancho‐Tello

Forriol²,

Gastaldi³

et al. 2015

Int J Artif Organs

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The Authors state that this manuscript has not been published previously and is not currently being assessed for publication by any journal other than The InternationalJournal of Artificial Organs.Each Author has contributed substantially to the research, preparation and production of the paper and approves of its submission to the Journal. FINANCIAL SUPPORT CONFLICT OF INTERESTThe authors state they do not have proprietary interest. MEETING PRESENTATIONThis study has been partially presented at the following meeting: ABSTRACT Purpose: Tissue engineering techniques were used to study cartilage repair over 12-month period in a rabbit model. Methods:A full-depth chondral defect along with subchondral bone injury were originated in the knee joint, where a biostable porous scaffold was implanted, synthesized of poly(ethyl acrylate-co-hydroxyethyl acrylate) copolymer. Morphological evolution of cartilage repair was studied 1 and 2 weeks, and 1, 3 and 12 months after implantation by histological techniques. Three-month group was chosen to compare cartilage repair to an additional group where scaffolds were preseeded with allogeneic chondrocytes before implantation, and also to controls, who underwent the same surgery procedure, with no scaffold implantation.

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Cellular interactions with synthetic polymer surfaces in culture

Cited by 242 publications

References 25 publications

Mammalian cell growth versus biofilm formation on biomaterial surfaces in an in vitro post-operative contamination model

Mammalian cell growth versus biofilm formation on biomaterial surfaces in an in vitro post-operative contamination model

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

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