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Bridge weigh in motion (B-WIM) uses accurate sensing systems to transform an existing bridge into a mechanism to determine actual traffic loading. This information on traffic loading can enable efficient and economical management of transport networks and is becoming a valuable tool for bridge safety assessment. B-WIM can provide site-specific traffic loading on deteriorating bridges, which can be used to determine if the reduced capacity is still sufficient to allow the structure to remain operational and minimise unnecessary replacement or rehabilitation costs and prevent disruption to traffic. There have been numerous reports on the accuracy classifications of existing B-WIM installations and some common issues have emerged. This paper details some of the recent developments in B-WIM which were aimed at overcoming these issues. A new system has been developed at Queens University Belfast using fibre optic sensors to provide accurate axle detection and improved accuracy overall. The results presented in this paper show that the fibre optic system provided much more accurate results than conventional WIM systems, as the FOS provide clearer signals at high scanning rates which require less filtering and less post-processing. A major disadvantage of existing B-WIM systems is the inability to deal with more than one vehicle on the bridge at the same time; sensor strips have been proposed to overcome this issue. A bridge can be considered safe if the probability that load exceeds resistance is acceptably low, hence B-WIM information from advanced sensors can provide confidence in our ageing structures.

This paper describes development of a contactless, low cost vision-based system for displacement measurement of civil structures. Displacement measurements provide a valuable insight into the structural condition and service behaviour of bridges under live loading. Conventional displacement gauges or GPS based systems have limitations in terms of access to the infrastructure and accuracy. The system introduced in this paper provides a low cost durable alternative which is rapidly deployable in the field and does not require direct contact or access to the infrastructure or its vicinity. A commercial action camera was modified to facilitate the use of a telescopic lens and paired with the development of robust displacement identification algorithms based on pattern matching. Performance was evaluated first in a series of controlled laboratory tests and validated against displacement measurements obtained using a fibre optic displacement gauge. The efficiency of the system for field applications was then demonstrated by capturing the validated bridge response of two structures under live loading including the iconic peace bridge. Located in the City of Derry, Northern Ireland, the Peace Bridge is a 310m curved self-anchored suspension pedestrian bridge structure. The vision-based results of the field experiment were confirmed against displacements calculated from measured accelerations during a dynamic assessment of the structure under crowd loading. In field applications the developed system can achieve a root mean square error (RMSE) of 0.03mm against verified measurements.

A procedure has been investigated for sorting viable cells according to their DNA content. Cells are stained with the U.V. activated fluorochromes 4'6-diamidino-2-phenylindole (DAPI), Hoechst 33258 or Hoechst 33342, and sorted with a Fluorescence Activated Cell Sorter. Hoechst 33342 is a suitable vital stain for a variety of cell types. Hoechst 33258 and DAPI, however, are quantitative vital stains for CHO cells only. Cloning efficiency is unaffected by the sorting procedure, and these stains are not mutagenic at concentrations suitable for vital staining. Potential applications of this procedure to cell biology are discussed.

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