Cellular internalization of doxorubicin loaded star-shaped micelles with hydrophilic zwitterionic sulfobetaine segments

Terminal modification of polymeric micelles with π-conjugated moieties for efficient anticancer drug delivery

“…The DLCs of the three mPEG-PCL-CIN, mPEG-PCL-COU and mPEG-PCL-CHR micelles were 17.7%, 20.5% and 25.5%, and the corresponding EEs were 92%, 90% and 93%. The DLC of mPEG-PCL-CHR micelles was nearly two folds higher than that of mPEG-PCL micelles, and 25.5% was an extremely high drug loading content to most of polymeric micelles [33,34]. The terminal modification of π-conjugated moieties could significantly improve the drug loading properties of mPEG-PCL micelles.…”

Self-assembled triangular DNA nanoparticles are an efficient system for gene delivery

“…Endocytosis pathways generally include clathrin-mediated endocytosis, caveolin-mediated endocytosis, clathrin-or caveolin-independent endocytosis, and macropinocytosis [44,45]. Endocytosis inhibitors are always applied to analyze the endocytosis pathways of cells, with appropriate concentrations and treatment time for minimize cytotoxicity [46]. LY-294002 and chlorpromazine were both found to significantly inhibit ssRNA-TNP uptake, suggested that nanoparticles entered the cells by macropinocytosis and clathrin-mediated endocytosis.…”

An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway

“…[29][30][31] Endocytosis inhibitors were usually applied to analyze the endocytosis pathways of cells, but their concentrations and treatment time should be well determined to minimize cytotoxicity. 32,33 Meanwhile, inhibition of certain endocytosis pathways may artificially upregulate other internalization routes that are not originally involved in the uptake of transfection particles because many chemical inhibitors are not specific to a single internalization pathway. 27 Therefore, inhibition experiments should be rationally designed to observe effects at a suitable time in referable parallel treatments.…”