Cellular invasion by Staphylococcus aureus involves a fibronectin bridge between the bacterial fibronectin-binding MSCRAMMs and host cell β1 integrins

Fowler, Trent; Wann, Elisabeth R.; Joh, Danny; Johansson, Staffan; Foster, Timothy J.; Höök, Magnus

doi:10.1078/0171-9335-00104

Cited by 239 publications

(208 citation statements)

References 43 publications

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“…Fn then serves as a molecular link connecting the bacteria with the principal host cell fibronectin receptor, the integrin a 5 h 1 (Fowler et al, 2000). Previously, we and others have observed that FnBP-initiated uptake via integrin a 5 h 1 is the major route of S. aureus internalization in vitro (Sinha et al, 1999;Sinha et al, 2000;Agerer et al, 2003).…”

Section: Evaluation Of Integrin-dependent Invasion Of S Aureus By Flmentioning

confidence: 99%

Quantification of bacterial invasion into adherent cells by flow cytometry

Pils

Schmitter

Neske

et al. 2006

Journal of Microbiological Methods

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Quantification of invasive, intracellular bacteria is critical in many areas of cellular microbiology and immunology. We describe a novel and fast approach to determine invasion of bacterial pathogens in adherent cell types such as epithelial cells or fibroblasts based on flow cytometry. Using the CEACAM-mediated uptake of Opa-expressing Neisseria gonorrhoeae as a well-characterized model of bacterial invasion, we demonstrate that the flow cytometry-based method yields results comparable to a standard antibiotic protection assay. Furthermore, the quantification of intracellular bacteria by the novel approach is not biased by intracellular killing of the microbes and correctly discriminates between cell-associated extracellular and bona fide intracellular bacteria. As flow cytometry-based quantification is also applicable to other pathogen-host interactions such as the integrinmediated internalization of Staphylococcus aureus, this approach provides a fast and convenient alternative for the quantification of bacterial uptake and should be particularly useful in elucidating the molecular mechanisms of pathogen-triggered host cell invasion. D

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Section: Evaluation Of Integrin-dependent Invasion Of S Aureus By Flmentioning

confidence: 99%

Quantification of bacterial invasion into adherent cells by flow cytometry

Pils

Schmitter

Neske

et al. 2006

Journal of Microbiological Methods

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“…100 However, many bacteria such as S. pneumoniae and Staphylococcus aureus utilize fibronectin to facilitate their internalization into epithelial cells. 101 Mutation of fbpA in L. monocytogenes resulted in a 100-fold decrease in bacterial counts in the intestine and liver in orally infected mice when compared with the wild-type strain after 72 h. 100 Furthermore, there was a 10-fold decrease in bacterial counts in the mesenteric lymph between mutant and wild-type but no difference in the spleenic bacterial counts. 100 This data indicated that FbpA is involved in the hepatic phases of listeriosis and represents a novel virulence factor.…”

Section: Listeria Monocytogenesmentioning

confidence: 99%

Signature tagged mutagenesis in the functional genetic analysis of gastrointestinal pathogens

Cummins

Gahan

2012

Gut Microbes

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Signature tagged mutagenesis is a genetic approach that was developed to identify novel bacterial virulence factors. It is a negative selection method in which unique identification tags allow analysis of pools of mutants in mixed populations. The approach is particularly well suited to functional genetic analysis of the gastrointestinal phase of infection in foodborne pathogens and has the capacity to guide the development of novel vaccines and therapeutics. In this review we outline the technical principles underpinning signature-tagged mutagenesis as well as novel sequencing-based approaches for transposon mutant identification such as TraDIS (transposon directed insertion-site sequencing). We also provide an analysis of screens that have been performed in gastrointestinal pathogens which are a global health concern (Escherichia coli, Listeria monocytogenes, Helicobacter pylori, Vibrio cholerae and Salmonella enterica). The identification of key virulence loci through the use of signature tagged mutagenesis in mice and relevant larger animal models is discussed.

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“…Streptococci and staphylococci express various fibronectin binding adhesins [7,[10][11][12][13][14]. The binding of pathogenic Streptococcus pyogenes and Staphylococcus aureus to epithelial cells via fibronectin facilitates their entry into cells [15][16][17]. The structural organization of the best characterized fibronectinbinding proteins of streptococci and staphylococci are similar [18].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of a gene for a fibronectin-binding protein of the oral bacterium Streptococcus mutans partially impairs its adherence to fibronectin

Miller-Torbert

Sharma

Holt

2008

Microbial Pathogenesis

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A sequence of 1,647 base pairs in length of S. mutans DNA that encodes for a 63 kDa protein with significant amino acid similarity with fibronectin-binding proteins of S. pyogenes and S. gordonii was cloned. The putative recombinant fibronectin-binding protein of S. mutans was purified using affinity chromatography and the cloned protein was used to prepare polyclonal antibodies against the recombinant protein. In immunoblot assays, antibodies against the S. pyogenes fibronectinbinding protein, FBP54, were cross-reactive with the S. mutans protein that was designated SmFnB. Additionally, antibodies to the S. mutans SmFnB protein reacted with the S. pyogenes FBP54 protein.The S. mutans SmFnB protein was found to bind to immobilized fibronectin in a concentration dependant manner. A mutant strain of S. mutans M51 that was constructed by alleleic exchange did not express the SmFnB protein. This mutant strain, S. mutans ΔSmFnB, was determined in an ELISA to bind to immobilized fibronectin 30% less when compared to the parental strain S. mutans M51. The results are consistent with the conclusion that the 63 kDa SmFnB protein of S. mutans is a fibronectin-binding protein that may contribute to the interaction of S. mutans with damaged heart tissue during pathogenesis of infective endocarditis. Also, the study suggests that multiple molecules may mediate the interaction of S. mutans with fibronectin.

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Cellular invasion by Staphylococcus aureus involves a fibronectin bridge between the bacterial fibronectin-binding MSCRAMMs and host cell β1 integrins

Cited by 239 publications

References 43 publications

Quantification of bacterial invasion into adherent cells by flow cytometry

Quantification of bacterial invasion into adherent cells by flow cytometry

Signature tagged mutagenesis in the functional genetic analysis of gastrointestinal pathogens

Inactivation of a gene for a fibronectin-binding protein of the oral bacterium Streptococcus mutans partially impairs its adherence to fibronectin

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