Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion of<i>Arabidopsis</i>Cellulose Synthase-Like A2 Inserted into Golgi Membrane

Caroli, Monica De; Lenucci, Marcello Salvatore; Sansebastiano, Gian Pietro Di; Tunno, Michela; Montefusco, Anna; Dalessandro, Giuseppe; Piro, Gabriella

doi:10.1155/2014/792420

Cited by 12 publications

(9 citation statements)

References 40 publications

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“…Projections of multiple z-stack confocal sections into one image were performed by generating a maximum intensity projection (Zen 2012 software, Zeiss). The power of laser line, the master gain, the digital gain, and the digital offset were identical for each experiment so that the images were comparable as reported in De Caroli et al (2014). Quantification of fluorescence was done using the ImageJ software.…”

Section: Methodsmentioning

confidence: 99%

Drought and Heat Differentially Affect XTH Expression and XET Activity and Action in 3-Day-Old Seedlings of Durum Wheat Cultivars with Different Stress Susceptibility

et al. 2016

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Heat and drought stress have emerged as major constraints for durum wheat production. In the Mediterranean area, their negative effect on crop productivity is expected to be exacerbated by the occurring climate change. Xyloglucan endotransglucosylase/hydrolases (XTHs) are chief enzymes in cell wall remodeling, whose relevance in cell expansion and morphogenesis suggests a central role in stress responses. In this work the potential role of XTHs in abiotic stress tolerance was investigated in durum wheat. The separate effects of dehydration and heat exposure on XTH expression and its endotransglucosylase (XET) in vitro activity and in vivo action have been monitored, up to 24 h, in the apical and sub-apical root regions and shoots excised from 3-day-old seedlings of durum wheat cultivars differing in stress susceptibility/tolerance. Dehydration and heat stress differentially influence the XTH expression profiles and the activity and action of XET in the wheat seedlings, depending on the degree of susceptibility/tolerance of the cultivars, the organ, the topological region of the root and, within the root, on the gradient of cell differentiation. The root apical region was the zone mainly affected by both treatments in all assayed cultivars, while no change in XET activity was observed at shoot level, irrespective of susceptibility/tolerance, confirming the pivotal role of the root in stress perception, signaling, and response. Conflicting effects were observed depending on stress type: dehydration evoked an overall increase, at least in the apical region of the root, of XET activity and action, while a significant inhibition was caused by heat treatment in most cultivars. The data suggest that differential changes in XET action in defined portions of the root of young durum wheat seedlings may have a role as a response to drought and heat stress, thus contributing to seedling survival and crop establishment. A thorough understanding of the mechanisms underlying these variations could represent the theoretical basis for implementing breeding strategies to develop new highly productive hybrids adapted to future climate scenarios.

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Section: Methodsmentioning

confidence: 99%

Drought and Heat Differentially Affect XTH Expression and XET Activity and Action in 3-Day-Old Seedlings of Durum Wheat Cultivars with Different Stress Susceptibility

et al. 2016

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“…Cellulose, hemicelluloses, and pectins are the main structural polysaccharides of the plant cell wall. Enzymes as glycosyl transferases and cellulose synthases are involved in the synthesis of these polysaccharides and other glycans (De Caroli et al 2014) and have been previously related with cold tolerance in young rice plants (Dametto et al 2015; Sperotto et al 2018; Kong et al 2019). To our knowledge, this is the first time that these cell wall-related genes are detected with higher expression in rice roots of CT plants when compared to CS ones.…”

Section: Discussionmentioning

confidence: 99%

Cold tolerance in rice plants is partially controlled by root responses

Rativa

Araújo

Friedrich

et al. 2020

Preprint

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“…The constructs expressing the fluorescent markers Aleu-GFP [ 15 ], ERD2-YFP [ 19 ], CslA2-GFP [ 20 ] and ST52-mCherry [ 21 ] were previously described. RFP-Chi is described here for the first time.…”

Section: Methodsmentioning

confidence: 99%

Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER

et al. 2021

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Plants rely on both actin and microtubule cytoskeletons to fine-tune sorting and spatial targeting of membranes during cell growth and stress adaptation. Considerable advances have been made in recent years in the comprehension of the relationship between the trans-Golgi network/early endosome (TGN/EE) and cytoskeletons, but studies have mainly focused on the transport to and from the plasma membrane. We address here the relationship of the cytoskeleton with different endoplasmic reticulum (ER) export mechanisms toward vacuoles. These emergent features of the plant endomembrane traffic are explored with an in vivo approach, providing clues on the traffic regulation at different levels beyond known proteins’ functions and interactions. We show how traffic of vacuolar markers, characterized by different vacuolar sorting determinants, diverges at the export from the ER, clearly involving different components of the cytoskeleton.

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Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane

Cited by 12 publications

References 40 publications

Drought and Heat Differentially Affect XTH Expression and XET Activity and Action in 3-Day-Old Seedlings of Durum Wheat Cultivars with Different Stress Susceptibility

Drought and Heat Differentially Affect XTH Expression and XET Activity and Action in 3-Day-Old Seedlings of Durum Wheat Cultivars with Different Stress Susceptibility

Cold tolerance in rice plants is partially controlled by root responses

Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER

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