Cellular metabolic responses of the marine diatom Pseudo-nitzschia multiseries associated with cell wall formation

Xu, Bin; Luo, Chun-Shan; Liang, Junrong; Chen, Dandan; Zhuo, Wen-Hao; Gao, Yahui; Chen, Changping; Song, Siyu

doi:10.1016/j.margen.2013.12.005

Cited by 5 publications

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transcripts for F-type ATP synthase subunits were also primarily found in module 1, suggesting increased ATP production in conjunction with a contig encoding a mitochondrial ATP/ADP carrier protein, also found in the module. One V-ATPase subunit was found in this module along with clathrin, two proteins that play an important role in diatom cell division and cell wall silicification (45, 46). However, most of the V-type ATPase subunits in the analysis were found in module 2, perhaps implying dynamic regulation of V-type ATPase subunits for different cellular functions throughout different phases of the bloom.…”

Section: Resultsmentioning

confidence: 99%

Molecular Forecasting of Domoic Acid during a Pervasive Toxic Diatom Bloom

Brunson,

Thukral,

Ryan

et al. 2023

Preprint

View full text Add to dashboard Cite

In 2015, the largest recorded harmful algal bloom (HAB) occurred in the Northeast Pacific, causing nearly 100 million dollars in damages to fisheries and killing many protected marine mammals. Dominated by the toxic diatomPseudo-nitzschia australis, this bloom produced high levels of the neurotoxin domoic acid (DA). Through molecular and transcriptional characterization of 52 near-weekly phytoplankton net-tow samples collected at a bloom hotspot in Monterey Bay, California, we identified active transcription of known DA biosynthesis (dab) genes from the three identified toxigenic species, includingP. australisas the primary origin of toxicity. Elevated expression of silicon transporters (sit1) during the bloom supports the previously hypothesized role of dissolved silica (Si) exhaustion in contributing to bloom physiology and toxicity. We find that co-expression of thedabAandsit1genes serves as a robust predictor of DA one week in advance, potentially enabling the forecasting of DA-producing HABs. We additionally present evidence that low levels of iron could have co-limited the diatom population along with low Si. Iron limitation represents a previously unrecognized driver of both toxin production and ecological success of the low iron adaptedPseudo-nitzschiagenus during the 2015 bloom, and increasing pervasiveness of iron limitation may fuel the escalating magnitude and frequency of toxicPseudo-nitzschiablooms globally. Our results advance understanding of bloom physiology underlying toxin production, bloom prediction, and the impact of global change on toxic blooms.SignificancePseudo-nitzschiadiatoms form oceanic harmful algal blooms that threaten human health through production of the neurotoxin domoic acid (DA). DA biosynthetic gene expression is hypothesized to control DA production in the environment, yet what regulates expression of these genes is yet to be discovered. In this study, we uncovered expression of DA biosynthesis genes by multiple toxigenicPseudo-nitzschiaspecies during an economically impactful bloom along the North American West Coast, and identified genes that predict DA in advance of its production. We discovered that iron and silica co-limitation restrained the bloom and likely promoted toxin production. This work suggests that increasing iron limitation due to global change may play a previously unrecognized role in driving bloom frequency and toxicity.

show abstract

Section: Resultsmentioning

confidence: 99%