Cellular pathways of the conducted electrical response in arterioles of hamster cheek pouch in vitro

Xia, Jun; Little, T. L.; Duling, Brian R.

doi:10.1152/ajpheart.1995.269.6.h2031

Cited by 104 publications

(132 citation statements)

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“…Evidence supporting the role of gap junctions includes the observation that conducted vasomotor responses in hamster cheek pouch are abolished or attenuated with the application of putative gap junction uncouplers (35,43). Moreover, electron microscopy has demonstrated that neighboring endothelial and smooth muscle cells in renal vasculature (29), thoracic aorta (36), and iridial arterioles (18) are connected by gap junctions, which render these cells electrically and chemically coupled (2,3,18,40,34,23,28,42,43). See Ref.…”

Section: Discussionmentioning

confidence: 99%

Autoregulation and conduction of vasomotor responses in a mathematical model of the rat afferent arteriole

Sgouralis

Layton

2012

American Journal of Physiology-Renal Physiology

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Steinhausen et al. (37) analyzed the propagation of vasomotor responses, induced by local electrical stimulation, in split hydronephrotic rat kidneys. Their results indicate that the responses decay with increasing distance from the stimulation site and that the decay is significantly faster upstream than downstream. An explanation for the asymmetric decay rates, which was elusive, is a motivation for the present study.In a previous study (5), we developed a detailed mathematical model of the myogenic response of a small segment of the afferent arteriole (AA) wall, including the endothelium and the surrounding smooth muscle cells. That model was used to examine the response of the AA segment to changes in mean and pulsatile pressure. Simulation results of that model are consistent with the hypothesis that the AA myogenic response plays an important role in protecting the glomerular capillaries against elevated systolic pressures. The goal of this study is to develop a multicell model of the AA by connecting a series of AA smooth muscle cells and endothelial cells via gap junction coupling and to use the model to study the myogenic response of the AA and its response to local electrical stimulation. The AA model is intended to be used as an essential component in models of integrated renal hemodynamic regulation. MATHEMATICAL MODELMulticell AA model. The model is an extension of our previous AA cell model (5) and represents a segment of an AA of length L (L ϳ300 m), consisting of a series of N cell ϭ 101 smooth muscle cell models (5), coupled via their gap junctions and via an endothelial cell layer. (An odd number of smooth muscle cell models were represented so that there is a middle cell that can be stimulated to study any asymmetry in the conduction of vasoconstrictive response.) The model AA segment is connected in series to a fixed resistor, denoted R end. The inflow pressure [P 0(t) at x ϭ 0] and the pressure at the end of the fixed resistor P end (at x ϭ 2L) are assumed to be known a priori. A schematic diagram is shown in Fig. 1. When the inflow pressure P 0 is varied or when a vasoconstrictive or vasodilative response is induced, the pressure at the end of the AA segment, which is denoted P(L, t) and which we refer to as the "outflow pressure," may also vary. We set P end to 0 mmHg and R end to equal the time-averaged value of the total resistance of the unstimulated AA with P 0 ϭ 100 mmHg, so that when the inflow pressure P 0 ϭ 100 mmHg, the outflow pressure P(L) Ϸ 50 mmHg.Each AA smooth muscle cell model incorporates the ionic transports, cell membrane potential, muscle contraction of the AA smooth muscle cells, and the mechanics of a thick-walled cylinder. The model represents the interaction of Ca 2ϩ and K ϩ fluxes mediated by voltagegated and voltage-calcium-gated channels, respectively, which gives rise to the periodicity of those transports. This results in a timeperiodic cytoplasmic calcium concentration, myosin light chains phosphorylation, and crossbridge formation with the attending muscle s...

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Section: Discussionmentioning

confidence: 99%

Autoregulation and conduction of vasomotor responses in a mathematical model of the rat afferent arteriole

Sgouralis

Layton

2012

American Journal of Physiology-Renal Physiology

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“…16,[25][26][27][28][29][30] In addition to current passing between cells through the gap junctions, there is also evidence for Ca 2+ signaling following elevations in SMC Ca 2+ by agonists such as phenylephrine and KCl, first reported in arterioles. 31 The consequent secondary rise in global endothelial Ca 2+ can enhance the production of both NO and EDH(F) and as a result influence vessel diameter.…”

Section: Dora Kamentioning

confidence: 99%

Coordination of Vasomotor Responses by the Endothelium

Dora

2010

Circ J

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“…With 2 minutes of rest in between, this stimulus was repeated at each distance in random order. 15,16 The impaled cell was identified by characteristic dye labeling (Figure 2). 6,9…”

Section: Experiments 1: Conducted Vasodilation and Hyperpolarizationmentioning

confidence: 99%

Endothelial Cell Pathway for Conduction of Hyperpolarization and Vasodilation Along Hamster Feed Artery

Emerson

Segal

2000

Circulation Research

224

327

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Abstract-Acetylcholine (ACh) evokes the conduction of vasodilation along resistance microvessels. However, it is not known which cell layer (endothelium or smooth muscle) serves as the conduction pathway. In isolated, cannulated feed arteries (Ϸ70 m in diameter at 75 mm Hg; length Ϸ4 mm) of the hamster retractor muscle, we tested the hypothesis that endothelial cells provide the pathway for conduction. Microiontophoresis of ACh (500 ms, 500 nA) onto the distal end of a feed artery evoked hyperpolarization (Ϫ13Ϯ2 mV) of both cell layers with vasodilation (15Ϯ1 m) along the entire vessel. To selectively damage endothelial cells (confirmed by loss of vasodilation to ACh and labeling of disrupted cells with propidium iodide), an air bubble was perfused through a portion of the vessel lumen, or a 70-kDa fluorescein-conjugated dextran (FCD) was illuminated within a segment (300 m) of the lumen. After endothelial cell damage, hyperpolarization and vasodilation conducted up to, but not through, the treated segment. To selectively damage smooth muscle cells (confirmed by loss of vasoconstriction to phenylephrine and labeling with propidium iodide), FCD was perifused around the vessel and illuminated. Vasodilation and hyperpolarization conducted past the disrupted smooth muscle cells without attenuation. We conclude that endothelial cells provide the pathway for conducting hyperpolarization and vasodilation along feed arteries in response to ACh. (Circ Res. 2000;86:94-100.)

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Cellular pathways of the conducted electrical response in arterioles of hamster cheek pouch in vitro

Cited by 104 publications

References 0 publications

Autoregulation and conduction of vasomotor responses in a mathematical model of the rat afferent arteriole

Autoregulation and conduction of vasomotor responses in a mathematical model of the rat afferent arteriole

Coordination of Vasomotor Responses by the Endothelium

Endothelial Cell Pathway for Conduction of Hyperpolarization and Vasodilation Along Hamster Feed Artery

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