Cellular Regulation of the Uterine Microenvironment That Enables Embryo Implantation

Zenclussen, Ana Claudia; Hämmerling, Günter J.

doi:10.3389/fimmu.2015.00321

Cited by 87 publications

(57 citation statements)

References 106 publications

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“…[112][113][114] The abundance of uNKs suggests an irremissible role for this unique cell population for reproductive processes. This challenging balance between immunity and specific tolerance requires a perfectly coordinated interplay of immune cells.…”

Section: Ma S T Cell S and Reproduc Ti Onmentioning

confidence: 99%

Mast cells—Good guys with a bad image?

Meyer

Zenclussen

2018

American J Rep Immunol

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Section: Ma S T Cell S and Reproduc Ti Onmentioning

confidence: 99%

Mast cells—Good guys with a bad image?

Meyer

Zenclussen

2018

American J Rep Immunol

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“…Endometrium is a vibrant tissue indulged in more than 400 cycles of regeneration and differentiation which is highly crucial during the reproductive period of women [1,2,6]. It bears a dynamic ability to generate an endometrial layer of 5-7mm thickness following menstruation.…”

Section: Discussionmentioning

confidence: 99%

“…It undergoes cyclic changes to proliferate and regenerate. It serves as an optimal environment for blastocyst implantation and as a site for placental generation [2]. Like any other organ in the human body, endometrium is susceptible to various disorders such as endometriosis, endometrial hyperplasia, endometritis, to name a few [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Marker Profiles of Human Endometrial Stem Cells at Various Passages Cultured In-Vitro

Kaingade

Nikam

Kulkarni

et al. 2017

JSRT

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Endometrium is a dynamic organ, which undergoes extensive proliferation and regeneration throughout reproductive cycle of women. Hence, extensive research on adult stem cells of endometrium is underway. An easy approach to identify stem cells is its marker characterization. However, does these markers behave same in all conditions of stem cells in-vitro is uncertain? Hence, the main objective of the study is to check if mesenchymal stem cells of the endometrium (eMSCs) could retain its marker characterization at various passages in-vitro.The study design was accomplished as follows: The endometrial tissue (n=10) were collected from reproductively active women with a mean age of 35±1.5 and body mass index of 24±1.4, undergoing D&C/hysterectomy. Samples were collected in a sterile manner. The plastic, adherent mesenchymal stem cells obtained from endometrial tissue (eMSCs) are characterized for markers such as CD44, CD166, CD106, CD49d, CD31, CD54, CD34, CD117, CD90, CD105, CD73, CD140b, ABCG2 at various passages (P1, P3, P5 & P10) in-vitro using flowcytometry. The obtained results were analysed statistically using student's t-test and significant results are discussed.The study identified tissue-specific markers of eMSCs, where the percentage expression of most of the markers was consistently similar at P3 and P10, expect for CD49d and CD54, which showed a reduction pattern and ABCG2, which showed an increase in percentage at P10 as compared to P3. This article reveals the significance of in-depth marker characterization of human endometrial stem cells in-vitro.

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“…It is postulated that almost 15% of couples are infertile in developed countries [1]. There had not been reported a substantial increase in demand for the management of infertility in the last decade [2]. Embryo implantation largely depends on the quality of the embryo and the=endometrial receptivity.…”

Section: Introductionmentioning

confidence: 99%

“…The receptive endometrium is defi ned as a healthy uterine milieu which support the transformation of endometrial cells into decidual cells, invasion of blastocysts, and fast growth of placenta [2]. This mechanism is helped by immune cells, growth factors, cytokines, and hormonal changes [3,4].…”

Section: Introductionmentioning

confidence: 99%

Effect of Intrauterine Infusion of Granulocyte Colony Stimulating Factor on IVF Outcomes in Infertile Women

Mostafa¹,

Farid²

2017

J Gynecol Res Obstet

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Objective: The primary goal of this study was to assess the G-CSF effects on IVF outcomes in women with normal endometrial thickness. Patients and Methods:This was a randomized controlled study performed at Assisted Reproductive Techniques Center of Ain Shams University Maternity Hospital, over a 2-year period, between Jan 2014 and Jan 2016, and included 60 infertile women with normal endometrial thickness. Women were scheduled for IVF and randomized into two groups. Exclusion criteria were positive history of repeated implantation failure (RIF), endocrine disorders, severe endometriosis, congenital or acquired uterine anomaly and contraindication for G-CSF (renal disease, sickle cell disease, or malignancy). In G-CSF group (n=30), 300 μg trans-cervical intrauterine of G-CSF was administered at the oocyte retrieval day. Controls (n=30) were treated with standard protocol. Chemical, clinical and ongoing pregnancy rates, implantation rate, and miscarriage rate were compared between groups.Results: Biochemical pregnancy had occurred in 27.3% of participants in group I and 21.3% of women in group II with a signifi cant difference between the two groups. The clinical pregnancy rate was 22% and 16% in group I and II respectively with no signifi cant difference between the two groups. The acceptability of women in group I was 68%, mild discomfort occurred in 44.7%, moderate discomfort was in 28.7%, severe discomfort was in 11.3% and 15.3% of women in group I had no discomfort at all. Diffi cult fl ushing had occurred in 14.7% of women. Conclusion:It looks that the clinical pregnancy rates were signifi cantly higher by intracavitary infusion of granulocyte colony stimulating factor

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Cellular Regulation of the Uterine Microenvironment That Enables Embryo Implantation

Cited by 87 publications

References 106 publications

Mast cells—Good guys with a bad image?

Mast cells—Good guys with a bad image?

Marker Profiles of Human Endometrial Stem Cells at Various Passages Cultured In-Vitro

Effect of Intrauterine Infusion of Granulocyte Colony Stimulating Factor on IVF Outcomes in Infertile Women

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