Cellular therapy for myocardial ischemia using a temperature-responsive biodegradable injectable polymer system with adipose-derived stem cells

Yoshizaki, Yuta; Takai, Hiroki; Mayumi, Nozomi; Fujiwara, Soichiro; Kuzuya, Akinori; Ohya, Yusuke

doi:10.1080/14686996.2021.1938212

Cited by 21 publications

(19 citation statements)

References 56 publications

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“…Animals C57BL/6N mice (9 week-old, male) were purchased from SHIMIZU Laboratory Supplies Co., Ltd. (Kyoto, Japan). AdSCs were collected from the mice as reported previously 43,49 . The experiments were performed for mice under anesthesia with iso urane using a small animal anesthesia station (DS Pharma Biomedical Co. Ltd., Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…IP hydrogel systems containing AdSCs have been investigated to achieve effective therapeutic results using AdSCs 2,47,48 . We also reported that covalent bond-forming IP systems containing AdSCs could effectively treat ischemic heart disease 49 .…”

Section: Introductionmentioning

confidence: 94%

“…AdSCs were collected from 9-week-old male C57BL/6N mice (SHIMIZU Laboratory Supplies Co., Ltd., Kyoto, Japan), as reported previously 43,49 . We then con rmed the expression of AdSC-speci c surface markers in these cells.…”

Section: Evaluation Of Surface Expression Markers For Adscmentioning

confidence: 99%

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Loss of pluripotency in adipose-derived stem cells after culture in temperature-responsive injectable polymer hydrogels

Mayumi

Murase

Yoshizaki

et al. 2022

Preprint

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Adipose-derived stem cells (AdSCs), a type of mesenchymal stem cells that are easily obtained from adipose tissue, are expected to be applicable to regenerative medicine and cellular delivery systems. Maintenance of cell pluripotency and control of the differentiation direction on the cellular scaffold are important for these applications. However, the differentiation directions of these cells are believed to depend on the physical properties of the scaffold. We investigated the conditions in which AdSCs could be cultured and maintain an undifferentiated state in injectable polymer (IP) hydrogels with various degrees of cross-linking. We also explored whether the pluripotency of the cells, that is, their ability to differentiate into multiple cells, is maintained by removing them from the IP hydrogels and inducing differentiation into osteoblasts and adipocytes. We confirmed that AdSCs cultured in IP hydrogels could maintain an undifferentiated state. However, their differentiation into osteoblasts and adipocytes cannot be ensured; specifically, pluripotency may decrease when AdSCs are cultured in IP hydrogels. When cultured in an IP hydrogel with extreme softness and poor cell adhesion properties, the AdSCs could remain in an undifferentiated state, but their pluripotency is reduced. These results will provide important insights into stem-cell delivery systems using IP hydrogels.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

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Loss of pluripotency in adipose-derived stem cells after culture in temperature-responsive injectable polymer hydrogels

Mayumi

Murase

Yoshizaki

et al. 2022

Preprint

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“…40 With a certain number of viable ADSCs retained inside, PEG hydrogels promoted ADSCs to secret VEGF for cardiac recovery post-MI. 172 4.2.4. Paracrine Effects of Stem Cells.…”

Section: Mesenchymal Stromal Cellsmentioning

confidence: 99%

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

et al. 2022

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With permanent heart muscle injury or death, myocardial infarction (MI) is complicated by inflammatory, proliferation and remodeling phases from both the early ischemic period and subsequent infarct expansion. Though in situ re-establishment of blood flow to the infarct zone and delays of the ventricular remodeling process are current treatment options of MI, they fail to address massive loss of viable cardiomyocytes while transplanting stem cells to regenerate heart is hindered by their poor retention in the infarct bed. Equipped with heart-specific mimicry and extracellular matrix (ECM)-like functionality on the network structure, hydrogels leveraging tissue-matching biomechanics and biocompatibility can mechanically constrain the infarct and act as localized transport of bioactive ingredients to refresh the dysfunctional heart under the constant cyclic stress. Given diverse characteristics of hydrogel including conductivity, anisotropy, adhesiveness, biodegradability, self-healing and mechanical properties driving local cardiac repair, we aim to investigate and conclude the dynamic balance between ordered architectures of hydrogels and the post-MI pathological milieu. Additionally, our review summarizes advantages of heart-tailored architectures of hydrogels in cardiac repair following MI. Finally, we propose challenges and prospects in clinical translation of hydrogels to draw theoretical guidance on cardiac repair and regeneration after MI.

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“…Various polymer hydrogels have been proposed as 3D cell scaffolds. [19][20][21][22][23][24][25][26][27][28] However, conventional polymer hydrogels are molecularly designed to enhance cell proliferation including cell adhesion. The cells adhered on conventional materials expressed inflammatory cytokines during the cell culture period.…”

Section: Introductionmentioning

confidence: 99%

Phospholipid polymer hydrogels with rapid dissociation for reversible cell immobilization

2022

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Cellular therapy for myocardial ischemia using a temperature-responsive biodegradable injectable polymer system with adipose-derived stem cells

Abstract: Ohya (2021): Cellular therapy for myocardial ischemia using a temperatureresponsive biodegradable injectable polymer system with adipose-derived stem cells, Science and Technology of Advanced Materials,

Cited by 21 publications

References 56 publications

Loss of pluripotency in adipose-derived stem cells after culture in temperature-responsive injectable polymer hydrogels

Loss of pluripotency in adipose-derived stem cells after culture in temperature-responsive injectable polymer hydrogels

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

Phospholipid polymer hydrogels with rapid dissociation for reversible cell immobilization

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