Cellular Uptake, Excretion and Localization of Hematoporphyrin Derivative (HPD)

Berns, Michael W.; Wile, Alan G.; Dahlman, Anton; Johnson, Fred M.; Burns, Robert; Sperling, Daryn; Guiltinan, Mark J.; Siemens, Ann; Walter, Robert; Hammer‐Wilson, Marie J.

doi:10.1007/978-1-4684-4406-3_14

Cited by 21 publications

(20 citation statements)

References 9 publications

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“…Similar observations have been made in Ref. 21. Signal intensities at characteristic points have been denoted by A, B, C, and D. For comparison, a corresponding spectrum from the other leg of the same rat is shown in Fig.…”

Section: Measurements and Resultsmentioning

confidence: 56%

Laser-Induced Fluorescence Studies of Hematoporphyrin Derivative (HPD) in Normal and Tumor Tissue of Rat

et al. 1984

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Fluorescence studies of hematoporphyrin derivative (HPD) in normal and tumor tissue of rat were performed with nitrogen laser excitation and optical multi-channel detection. Fifteen types of tissue including inoculated tumor were investigated for rats at different delays after HPD injection. Optimum contrast functions and other criteria for discriminating tumor tissue from normal tissue are discussed. The results should have implications for practical human HPD endoscopy.

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Section: Measurements and Resultsmentioning

confidence: 56%

Laser-Induced Fluorescence Studies of Hematoporphyrin Derivative (HPD) in Normal and Tumor Tissue of Rat

et al. 1984

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“…This would occur if the excited triplet state of Protophorphrin IX has a higher probability of interacting with molecular oxygen to produce singlet oxygen as opposed to the excited Protophorphrin IX giving up its energy via fluorescence. Generally, the mechanism of PDT at the cellular level is thought to be generation of singlet oxygen in association with membrane-rich cytoplasm organelles such as mitochondria [15] and lysosomes [16]. There have been several studies examining possible PDT effects on the nucleus, cell division and the cytoskeleton [17,[19][20][21][22].…”

Section: Discussionmentioning

confidence: 99%

“…It was surprising to find such a high nuclear sensitivity because the highest level of sensitizer fluorescence was found in the cytoplasm around the nucleus. In addition, the vast majority of published cell studies have demonstrated that cellular phototoxicity is likely caused by the generation of reactive singlet oxygen by photosensitizers associated with organelles such as the lysosomes and mitochondria [15,16]. Notwithstanding this large body of literature, there are also several studies that demonstrate a PDT effect on DNA, chromosomes and the mitotic spindle (see the excellent review by Moan et al [17]).…”

Section: Introductionmentioning

confidence: 99%

Chromosomes are target sites for photodynamic therapy as demonstrated by subcellular laser microirradiation

Luan

Kasravi

et al. 2000

Journal of Photochemistry and Photobiology B: Biology

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“…Bilirubin can abolish respiratory control, uncouple oxidative phosphorylation, and induce mitochondrial swelling in liver, heart, brain tissues (26), and cultured cells (27); it has also been shown to lower mitochondrial ATP and electrolyte content in renal slices (28) and to inhibit superoxide production by polymorphonuclear cells (29). Hematoporphyrins are potent cytocidal agents in tumor phototherapy (30, 3 1) but can also inhibit cartilage metabolism (32) and cell proliferation (33,34) in the absence of light. Finally, bile pigments are vital to lower organisms, being responsible for the spectrum of photosynthetic energy transfer reactions (17).…”

Section: Discussionmentioning

confidence: 99%

Bilirubin and Heme as Growth Inhibitors of Chicken Embryos in Ovo

1990

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ABSTRACT. The increased morbidity during pregnancies complicated by hematologic or liver disease has generally been attributed to the metabolic abnormalities of the illness itself. Because tetrapyrrole concentrations are elevated in these conditions, we introduced bilirubin or heme (prepared as 10 mM solutions) into the air sac of fertilized chicken eggs to study their effect on the growth of normal chicken embryos. In 9-d eggs, the injection of 0.06 mL heme resulted in significant embryo growth inhibition as indicated by overall wt (91 2 3% versus control), tibia length (84 f 2%), tibia wt (81 + 3%), femur length (88 + I%), and femur wt (78 + 3%); doses greater than 0.10 mL resulted in substantial fetal losses. The injection of 0.06 mL bilirubin into the same-age eggs also resulted in less than normal tibia length (87 2 2% versus control), tibia wt (75 2 4%), femur length (91 + 2%), and femur wt (81 2 3%); larger doses resulted in more pronounced growth inhibition, but fetal losses were less common than with heme. Older chick embryos (12-d) appeared more resistant to the effects of bilirubin: 0.15 mL bilirubin inhibited only tibia and femur wt; larger doses were required to significantly suppress the other growth parameters. The sameage chicken embryos, however, remained exquisitely sensitive to heme; 0.05 mL resulted in less than normal whole embryo wt (88 + 2% versus control), tibia length (80 2 I%), tibia wt (76 +. I%), femur length (78 + I%), and femur wt (77 + 1%). Substantial fetal loss occurred with heme doses above 0.10 mL as well as among the less mature chicks. Significant inhibition of the growth of long bones (tibia and femur) also occurred when we added 0.05 mM bilirubin or 0.025 mM Hb to bones maintained in organ culture in vitro for 5 d. We conclude that several heme compounds and bilirubin inhibit the growth and survival of normal chick embryos in ovo and in vitro.We suggest that prenatal exposure to high levels of these compounds may have a previously underestimated negative influence on fetal development (Pediatr Res 27: 617-621, 1990) In pregnancies complicated by maternal hematologic or liver diseases, the risk of adverse fetal outcome is higher than normal. How these maternal illnesses harm the fetus remains poorly understood (1-3). Although maternal hematologic or liver diseases are systemic disorders with multiple metabolic abnormalities that could affect fetal outcome, one feature they share is sustained elevation of circulating tetrapyrroles (heme compounds and/or bilirubin), which may affect fetal metabolism directly. Supported by NIH Grant AR 37326. 6That exposure to heme compounds or bilirubin may exert a direct negative influence on fetal growth and development has not received attention, but our recent studies prompt us to examine this possibility carefully. We have demonstrated that several tetrapyrroles can profoundly inhibit the growth of embryonic chicken cartilage in organ culture in vitro (4-7), the growth of normal, growing rats in vivo (8), and the proliferation of chon...

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Cellular Uptake, Excretion and Localization of Hematoporphyrin Derivative (HPD)

Cited by 21 publications

References 9 publications

Laser-Induced Fluorescence Studies of Hematoporphyrin Derivative (HPD) in Normal and Tumor Tissue of Rat

Laser-Induced Fluorescence Studies of Hematoporphyrin Derivative (HPD) in Normal and Tumor Tissue of Rat

Chromosomes are target sites for photodynamic therapy as demonstrated by subcellular laser microirradiation

Bilirubin and Heme as Growth Inhibitors of Chicken Embryos in Ovo

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