Cellulase and Bacterial Inoculant Effects on Cocksfoot and Lucerne Ensiled at High Dry Matter Levels

Nadeau, Elisabet; Buxton, D. R.

doi:10.1002/(sici)1097-0010(199703)73:3<369::aid-jsfa743>3.0.co;2-g

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…The lack of response to enzyme treatment obtained in some studies has been attributed to enzyme instability (Moharrery et al 2009), as temperature, pH, and ionic strength can affect the activity of enzymes. The high lignin concentration in lucerne has been reported to be responsible for its recalcitrance towards enzymatic attack by cellulase during the ensiling process (Nadeau and Buxton 1997;Moharrery et al 2009). In general, the reasons for the above mentioned inefficient results are complex and differed among experiments, which may explain among silages treated in this research, C alone treatment had no significant effect for silage fermentation and IVDMD.…”

Section: Discussionmentioning

confidence: 72%

“…Alternatively, Stokes et al (1992) found that combination of LAB and cellulase was antagonistic and, therefore, did not improve silage quality. Nadeau and Buxton (1997) also reported that the effect of this mixture on silage quality varied among plant species, with cocksfoot being generally more responsive to cellulase than alfalfa.…”

Section: Introductionmentioning

confidence: 95%

“…The second cut of alfalfa herbage was hand-harvested with a sickle at the early blooming stage, August 5, 2016. The forage samples were cut to approximately 2 cm length using a forage cutter (Lingong Machinery, Shandong, China) and wilted for 12 h to obtain the recommended dry matter (DM) content of approximately 400 g kg -1 fresh matter (FM) prior to ensiling (Nadeau and Buxton 1997). Baseline chemical and microbiological characteristics of the pre-ensiled alfalfa samples are shown in Table 2.…”

Section: Silage Preparationmentioning

confidence: 99%

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Influence of lactic acid bacteria, cellulase, cellulase-producing Bacillus pumilus and their combinations on alfalfa silage quality

Zhang

et al. 2018

Journal of Integrative Agriculture

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This study assessed the effects of lactic acid bacteria (LAB), cellulase, cellulase-producing Bacillus pumilus and their combinations on the fermentation characteristics, chemical composition, bacterial community and in vitro digestibility of alfalfa silage. A completely randomized design involving a 8 (silage additives)×3 or 2 (silage days) factorial arrangement of treatments was adopted in the present study. The 8 silage additive treatments were: untreated alfalfa (control); two commercial additives (GFJ and Chikuso-1); an originally selected LAB (Lactobacillus plantarum a214) isolated from alfalfa silage; a cellulase-producing Bacillus (CB) isolated from fresh alfalfa; cellulase (C); and the combined additives (a214+C and a214+CB). Silage fermentation characteristics, chemical composition and microorganism populations were analysed after 30, 60 and 65 days (60 days followed by exposure to air for five additional days). In vitro digestibility was analysed for 30 and 60 days. Compared with the other treatments, selected LAB a214, a214 combined with either C or CB, and Chikuso-1 had the decreased (P<0.001) pH and increased (P<0.001) lactic acid concentrations during the ensiling process, and there were no differences (P>0.05) among them. Fiber degradation was not significant (P≥0.054) in any C or CB treatments. The a214 treatment showed the highest (P=0.009) in vitro digestibility of dry matter (595.0 g kg-1 DM) after ensiling and the highest abundance of Lactobacillus (69.42 and 79.81%, respectively) on days 60 and 65, compared to all of other treatments. Overall, the silage quality of alfalfa was improved with the addition of a214, which indicates its potential as an alfalfa silage inoculant.

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Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 95%

Section: Silage Preparationmentioning

confidence: 99%

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Influence of lactic acid bacteria, cellulase, cellulase-producing Bacillus pumilus and their combinations on alfalfa silage quality

Zhang

et al. 2018

Journal of Integrative Agriculture

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“…Conversely, other studies have shown a positive effect of enzyme treatment on the fermentation characteristics of lucerne silage (Jaster and Moore, 1988;Sheperd et al, 1995;Nadeau and Buxton, 1997). However, in these studies a concomitant decrease in the fibre content of the silages was also noted.…”

Section: Silage Fermentation Characteristicsmentioning

confidence: 68%

The effect of enzyme treatment on the nutritive value of lucerne for equids

et al. 2007

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“…Similarly, Nadeau and Buxton (1997) found that inoculants had no effect on the ammonia-N concentration in alfalfa silage. When Keady and Steen (1994) added L. plantarum bacteria at 10 9 cfu/g to silage at the time of feeding, they found no response in terms of intake, digestibility, or rumen fermentation.…”

Section: Muckmentioning

confidence: 97%

Rumen fermentation and digestibility of spent mushroom (Pleurotus ostreatus) substrate inoculated with Lactobacillus brevis for Hanwoo steers

Baek

Kim

Reddy

et al. 2017

Rev Colomb Cienc Pecu

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Original articles 267Rumen fermentation and digestibility of spent mushroom (Pleurotus ostreatus) substrate inoculated with Lactobacillus brevis for Hanwoo steers ¤ Fermentación ruminal y digestibilidad de la cama de champiñón (Pleurotus ostreatus) desechada inoculada con Lactobacillus brevis en novillos Hanwoo Fermentação ruminal e digestibilidade de resíduo de substrato de cogumelo (Pleurotus ostreatus) inoculado com Lactobacillus brevis en novilhos Hanwoo AbstractBackground: Ensiling spent mushroom substrate (SMS) generally increases its nutrient digestibility and quality. Objective: To determine the feed quality and digestibility of SMS from Pleurotus ostreatus (SMSP) inoculated with lactic acid bacteria (LAB: Lactobacillus brevis) in Hanwoo steers. Methods: Ruminal disappearance of SMSP and inoculated SMSP (ISMSP) were evaluated in three rumen-fistulated Hanwoo steers (408 ± 13.0 Kg body weight). Further, three healthy Hanwoo steers (336 ± 69.0 Kg body weight) were randomly allotted to one of three dietary treatments (control: 25% straw, 75% concentrates; treatments: 25% straw, 60% concentrates, and 15% of either SMSP or ISMSP) in a 3×3 Latin square design. Results: The 268Rev Colomb Cienc Pecu 2017; 30:267-277 Baek YC et al. Inoculation of spent mushroom with Lactobacillus in steerschemical composition of the ISMSP diet did not differ from that of the control or the SMSP diets. In the ISMSP diet, the rate of decrease of pH of ruminal fluid and the increase in storage period was greater than with the SMSP diet. Ruminal disappearance of dry matter, crude protein, neutral detergent fiber, and acid detergent fiber were slightly higher in steers fed ISMSP than those fed SMSP. Furthermore, the degradation rate and effective degradability of crude protein was greater in the ISMSP diet than in the SMSP diet. Effective ruminal fermentation characteristics and total nutrients digestibility were not affected by SMSP nor ISMSP diet. Conclusion: The SMSP and ISMSP diets could replace formulated concentrate without adverse effects and be a cost-effective feed for Hanwoo steers. Furthermore, LAB inoculation improved the SMSP preservation.Keywords: agricultural by-products ensilage, feed cost, nylon bag technique, total-tract digestibility, volatile fatty acid. ResumenAntecedentes: El ensilado de cama de champiñón desechada (SMS) generalmente aumenta la digestibilidad y la calidad de sus nutrientes. Objetivo: Determinar la calidad del alimento y digestibilidad del SMS a partir de Pleurotus ostreatus (SMSP) inoculado con bacterias ácido-lácticas (LAB: Lactobacillus brevis) en bueyes Hanwoo. Métodos: La desaparición ruminal del SMSP y ISMSP (SMSP inoculado) fue evaluada en tres bueyes Hanwoo fistulados en el rumen (408 ± 13,0 Kg peso corporal). Igualmente, tres bueyes Hanwoo sanos (336 ± 69,0 Kg peso corporal) fueron asignados al azar a uno de los tres tratamientos dietéticos (control: 25% de heno, 75% de concentrados; tratamientos: 25% de heno, 60% de concentrados y 15% de SMSPo ISMSP) en un diseño cuadrado latino 3×3. Re...

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Cellulase and Bacterial Inoculant Effects on Cocksfoot and Lucerne Ensiled at High Dry Matter Levels

Cited by 6 publications

References 21 publications

Influence of lactic acid bacteria, cellulase, cellulase-producing Bacillus pumilus and their combinations on alfalfa silage quality

Influence of lactic acid bacteria, cellulase, cellulase-producing Bacillus pumilus and their combinations on alfalfa silage quality

The effect of enzyme treatment on the nutritive value of lucerne for equids

Rumen fermentation and digestibility of spent mushroom (Pleurotus ostreatus) substrate inoculated with Lactobacillus brevis for Hanwoo steers

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