Cellulase interacts with Lactobacillus plantarum to affect chemical composition, bacterial communities, and aerobic stability in mixed silage of high-moisture amaranth and rice straw

Mu, Lin; Xie, Zhan; Hu, Longxing; Chen, Guihua; Zhang, Zhifei

doi:10.1016/j.biortech.2020.123772

Cited by 98 publications

(124 citation statements)

References 41 publications

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“…The LP inoculation also resulted in a higher content of LA and lower content of AA and BA in LPGP, due to the enhanced homolactic fermentation. Consequently, more organic matters would be preserved, which was consistent with increasing DM content in this study [2,9]. Flieg's score based on the organic acid ingredient was reported as the important evaluation proxy for silage quality, which could indicate the odor characteristic in a way [4,28].…”

Section: The Effects Of Lp Inoculation On Physicochemical Properties Of Silagesupporting

confidence: 82%

“…Moreover, the LP inoculation did not facilitate the accumulation of CF and CP, while it basically maintained the value of these two important nutrition indicators (Table 3). The ADF and NDF content declined during the silage; in particular, the ADF content significantly declined at the end of co-ensiling (p < 0.05), which was different from Mu et al's report that LP played no significant role in ADF and NDF content in amaranth and rice straw silage [9]. This different result could account for more organic acid to accelerate the lignocellulose hydrolysis and the different raw materials in the silage study [27].…”

Section: The Effects Of Lp Inoculation On Physicochemical Properties Of Silagecontrasting

confidence: 62%

“…Vegetable waste, such as cauliflower and cabbage leaf byproducts, are ubiquitous in landfills but rarely reported to be bio-transformed as animal feed, such as silage [2]. LP could be an efficient microbial inoculation to enhance the fermentation quality of ensiling different materials [2,9,11,25,26] but the underlying effect and intricate relationships have been little investigated. This present study disposed of cabbage byproducts as added-value silage and was the first to focus on the underlying effect of LP improving silage quality and microbial community functional diversity in food microbial areas.…”

Section: Discussionmentioning

confidence: 99%

“…The minimum DM (>200 g•kg −1 FM) and WSC content (>50 g•kg −1 DM) were reported necessary as LABs transformed the WSC to organic acid, e.g., LA, to decrease the pH and preserve the forage successfully [9,27]. Thus, the formula of initial materials was suitable in this study.…”

Section: The Effects Of Lp Inoculation On Physicochemical Properties Of Silagementioning

confidence: 96%

“…Hence, inoculation of exogenous LP could be a feasible method to transform cabbage waste and rice straw into animal feed. The inoculation significantly reduced ammonia-N content, which could be explained by the fact that more LABs and LA could effectively restrain undesirable bacteria such as Enterobacteria from degrading protein and oligopeptide to ammonia [6,9]. Moreover, ammonia-N below 7% and beyond 10% indicated a successful silage fermentation and severe nutritional loss, respectively [29].…”

Section: The Effects Of Lp Inoculation On Physicochemical Properties Of Silagementioning

confidence: 99%

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Keystone Taxa Lactiplantibacillus and Lacticaseibacillus Directly Improve the Ensiling Performance and Microflora Profile in Co-Ensiling Cabbage Byproduct and Rice Straw

Zhang

Shi

et al. 2021

Microorganisms

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Ensiling has been widely applied to cope with agricultural solid waste to achieve organic waste valorization and relieve environmental pressure and feedstuff shortage. In this study, co-ensiling of cabbage leaf byproduct and rice straw was performed with inoculation of Lactiplantibacillusplantarum (LP) to investigate the effects of inoculation on ensiling performance and microflora profiles. Compared to the control, LP inoculation preserved more dry matter (DM) content (283.4 versus 270.9 g·kg−1 fresh matter (FM) on day 30), increased lactic acid (LA) content (52.1 versus 35.8 g·kg−1 dry matter on day 15), decreased pH (3.55 versus 3.79 on day 15), and caused accumulation of acetic acid (AA), butyric acid (BA), and ammonia. The investigation showed that LP inoculation modified microflora composition, especially resisting potential pathogens and enriching more lactic acid bacteria (LAB) (p < 0.05). Moreover, Lactiplantibacillus and Lacticaseibacillus were identified as the keystone taxa that influenced physicochemical properties and interactions in microflora. They were also the main functional species that directly restrained undesirable microorganisms (p < 0.05), rather than indirectly working via metabolite inhibition and substrate competition (p > 0.05). The results of this present study improve the understanding of the underlying effect of LP inoculation on improving silage quality and facilitate the bio-transformation of cabbage byproduct and rice straw as animal feed.

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Section: The Effects Of Lp Inoculation On Physicochemical Properties Of Silagesupporting

confidence: 82%

Section: The Effects Of Lp Inoculation On Physicochemical Properties Of Silagecontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: The Effects Of Lp Inoculation On Physicochemical Properties Of Silagementioning

confidence: 96%

Section: The Effects Of Lp Inoculation On Physicochemical Properties Of Silagementioning

confidence: 99%

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Keystone Taxa Lactiplantibacillus and Lacticaseibacillus Directly Improve the Ensiling Performance and Microflora Profile in Co-Ensiling Cabbage Byproduct and Rice Straw

Zhang

Shi

et al. 2021

Microorganisms

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Lactobacillus plantarum and molasses alter dynamic chemical composition, microbial community, and aerobic stability of mixed (amaranth and rice straw) silage

Xie

et al. 2021

J Sci Food Agric

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BACKGROUND The objective was to determine how molasses and Lactobacillus plantarum affect chemical composition, fermentation quality, aerobic stability, and the microbial community of an ensiled mixture of amaranth (Amaranthus hypochondriaus, AF) and rice straw. Treatments were control (C, no addition), L. plantarum (L; 2 × 105 cfu g−1 fresh weight), molasses (M; 40 g kg−1 fresh matter), and their combination (LM). All treatments were ensiled for 1, 3, 5, 7, and 30 days. RESULTS All additives improved fermentation quality with greater lactic acid (LA), acetic acid, and lower pH than C silage over the ensiling period. The LM silage combination optimized fermentability, manifested as greater LA contents and a more rapid pH reduction during the first 7 days of ensiling than L or M silages. After 30 days of ensiling, inoculant L. plantarum increased Lactobacillus abundance and reduced bacterial diversity and Enterobacteriaceae abundance compared with silage treated with molasses. Molasses addition reduced the relative concentration of structural carbohydrates (neutral and acid detergent fiber, and hemicellulose) after 30 days of ensiling. Finally, there was spoilage after 2 days and 4 days of aerobic exposure in C and LM silages respectively, whereas L silage had not spoiled after 4 days. CONCLUSIONS Although the combination of L. plantarum and molasses further optimized fermentation characteristics, L silage had better aerobic stability. © 2021 Society of Chemical Industry.

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Microbial community structure, co‐occurrence network and fermentation characteristics of woody plant silage

Lin

Sun

et al. 2021

J Sci Food Agric

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BACKGROUND: Feed shortage is a factor restricting animal production in the tropics, therefore how to use natural woody plant resources as animal feed is an important strategy.RESULTS: Under the dual stress of an anaerobic and acidic environment, the microbial response during the fermentation of paper mulberry (PM) silage was found to be sensitive. The Gram-negative bacteria and mould died, and the dominant microbial community rapidly shifted to Gram-positive bacteria, resulting in a large reduction in microbial diversity and abundance. Exogenous bran additives interfered with the stress effects of the woody silage environment. Wheat bran (WB) accelerated the response of microorganisms to the anaerobic stress, and lactic acid bacteria became the dominant microbial community, thereby enhancing the lactic acid fermentation of silage, affecting the metabolic pathways of microorganisms, and improving the flavour and quality of the silage. Addition of rice bran made Enterobacter and Clostridium species quickly respond to the stress of the silage environment and become the predominant bacterial groups. In particular, anaerobic and spore-forming Clostridium species showed a strong tolerance to the silage environment, leading to butyric acid fermentation and protein degradation of the silage, and reducing its fermentation quality.CONCLUSION: The PacBio single-molecule real-time (SMRT) sequencing technology accurately revealed the microbial cooccurrence network and fermentation mechanism of silage. Our results indicate that PM can be used in combination with WB to prepare high-quality silage for animal production.

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Cellulase interacts with Lactobacillus plantarum to affect chemical composition, bacterial communities, and aerobic stability in mixed silage of high-moisture amaranth and rice straw

Cited by 98 publications

References 41 publications

Keystone Taxa Lactiplantibacillus and Lacticaseibacillus Directly Improve the Ensiling Performance and Microflora Profile in Co-Ensiling Cabbage Byproduct and Rice Straw

Keystone Taxa Lactiplantibacillus and Lacticaseibacillus Directly Improve the Ensiling Performance and Microflora Profile in Co-Ensiling Cabbage Byproduct and Rice Straw

Lactobacillus plantarum and molasses alter dynamic chemical composition, microbial community, and aerobic stability of mixed (amaranth and rice straw) silage

Microbial community structure, co‐occurrence network and fermentation characteristics of woody plant silage

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