Cellulase Production by Aspergillus niger ATCC 16888 on Copra Waste from Coconut Milk Process In Layered Packed-bed Bioreactor

Abstract

Solid-state fermentation (SSF) has been used to produce value-added products from agricultural and food processing wastes, but the majority of SSF production has been conducted on a small scale due to the limitations of heat and mass transfer. This research aimed to reduce the temperature changes in packed-bed bioreactor during the cellulase fermentation of Aspergillus niger ATCC 16888 on copra waste from coconut milk process. The three configurations of bioreactor used in this study consisted of one-(control), two-and three-layered packed-bed bioreactors. Total volumes of packed-bed bioreactor and bed material were 127 and 57 L, respectively. The sterilized air 30 °C was forced from the bottom of bioreactor at 0.20 vvm. The highest cellulase production (5.0 ± 0.4 FPU g-1 DS) was obtained from the bottom zone of the three-layered bioreactor after fermenting for 1 d. However, the greatest growth was found at the top one, in which a large amount of aerial mycelium was detected.

Genome sequences of 24 Aspergillus niger sensu stricto strains to study strain diversity, heterokaryon compatibility, and sexual reproduction

“…A. niger CBS101883 (formerly known as A. lacticoffeatus ) has not been studied extensively, however it has been used for β-glucosidase production ( Cardoso et al 2017 ). The type strain A. niger CBS554.65 has not been extensively studied either, although some examples of researched enzyme production exist ( Gera et al 2008 ; Chysirichote 2018 ). Taken together, this clade contains most if not all A. niger sensu stricto strains available from literature used as protein and enzyme producers by industry.…”

High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

“…However, most of the studies on SSF thus far have been based on experiments on a laboratory scale, such as in Erlenmeyer flasks, 23 glass columns, 24 plate bioreactors, 25 and bag bioreactors, 26 because of the accumulation of heat generated by microbial respiration and other metabolisms that causes scale-up problems. 27,28 According to a previous work, 29 spent copra from coconut milk was suitable for cellulase production by Trichoderma reesei; however, the cellulase production from the SSF of spent copra was enhanced by supplementing WB as an organic nitrogen source. To scale up the SSF of spent copra for cellulase production, a multi-layered packed-bed bioreactor was used to remove the accumulated heat during SSF, 29 which was the major problem of scaling up the bioreactor.…”

“…According to a previous work, spent copra from coconut milk was suitable for cellulase production by Trichoderma reesei; however, the cellulase production from the SSF of spent copra was enhanced by supplementing WB as an organic nitrogen source. To scale up the SSF of spent copra for cellulase production, a multi-layered packed-bed bioreactor was used to remove the accumulated heat during SSF, which was the major problem of scaling up the bioreactor. , Even though dividing a packed bed into many layers helped to increase the heat removal and enzyme production, the uniformity of enzyme and biomass production was not achieved. This research aimed to study the cellulase and xylanase production from spent copra by the SSF of T.…”