Cellulase production by Penicillium echinulatum on lactose

Abstract

The inducer effect of lactose on cellulase activity in Penicillium echinulatum 9A02S1 was studied. Submerged cultivation was performed using different concentrations of lactose and cellulose, in which the pH, mycelial mass, soluble proteins, filter paper activity (FPA), and activity of beta-glucosidases were measured. The cultures containing lactose only presented low FPAs (0.1 FPU/ml). The cultures with associated cellulose and lactose and those containing cellulose only presented similar enzymatic activities (1.5 FPU/ml), suggesting the possibility of up to 75% reduction in the cellulose concentration. In relation to the beta-glucosidases, increasing the lactose/cellulose ratio results in a proportional increase of enzymatic activity. In the cultures using both inducers, there is a longer duration of the acid phase in relation to treatments using only cellulose or lactose, indicating diauxia and catabolic repression.

Isolation of Uracil Auxotrophs of the FungusAcremonium cellulolyticusand the Development of a Transformation System with thepyrFGene

“…[4][5][6] The cellulase produced by fungi has three major enzyme components: endoglucanases, which randomly hydrolyze internal glycosidic linkages; cellobiohydrolases, which produce cellobiose from cellulose chain ends; and -glucosidases, which convert cellobiose into glucose. 6) The filamentous fungus, Acremonium cellulolyticus, which was isolated in 1982 from soil in Japan, is a cellulose-degrading organism.…”

Carbon source directs the differential expression of β-glucosidases in Stachybotrys microspora

“…The inducer of cellulase production is mainly cellulose (Suto and Tomita 2001) and its derivatives (CMC, HEC) and other oligosaccharides, disaccharides (sophorose, gentiobiose, laminaribiose, lactose) (Suto and Tomita 2001;Sehnem et al 2006) and even monosaccharides like xylose (Suto and Tomita 2001;Smaali et al 2004). In contrast, cellulases, as many other hydrolases, are repressed by glucose (Suto and Tomita 2001) and other simple sugars.…”

Fermentation Optimization and Unstructured Kinetic Model for Cellulase Production by Rhizopus stolonifer var. reflexus TP-02 on Agriculture By-Products

“…Hence, rice straw grindings filtered by 80 mesh were selected for further experiments. In addition, lactose, which was found to be a good carbon source and inducer for cellulase production by some fungi [39][40][41], was tested in the presence of rice straw. However, no obvious inducer effect was observed in the fermentation of R. stolonifer TP-02 by addition of lactose.…”