Cellulase production by thermophilic Bacillus sp. SMIA-2 and its detergent compatibility

Ladeira, Silvânia Alves; Cruz, Erica; Delatorre, Andréia Boechat; Barbosa, João Batista; Martins, Meire Lélis Leal

doi:10.1016/j.ejbt.2014.12.008

Cited by 126 publications

(102 citation statements)

References 26 publications

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“…Immanuel et al [43] reported that cellulose quality, temperature, aeration, carbon sources, incubation period, medium additives, pH of the medium and presence of inducers are important parameters for the optimized production of cellulase enzymes. Other researchers [21,[44][45][46] have also reported cellulase production enhancement by the optimization of cultural conditions. The optimum temperature, pH and nitrogen source for cellulase production were 40 o C, 7 and yeast extract for both substrates.…”

Section: Discussionmentioning

confidence: 99%

Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks

Ezebuiro¹,

Ogugbue²,

Oruwari³

et al. 2015

J Biotechnol Biomater

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Bioethanol production potential of ethanol-tolerant Bacillus cereus strain GBPS9 using sugarcane bagasse and cassava peels as feedstocks was investigated. The Bacillus cereus GBPS9 used in this study was isolated from agro-wastes impacted soil and classified based on phylogenetic analysis of its 16S rRNA gene. The sequence of the isolate has been deposited in GenBank under the accession number KT318371.1. The isolate was selected based on its cellulolytic ability, tolerance to ethanol concentration of 6% (v/v) and ability to ferment sugar to ethanol. The substrates employed in the study were cassava peels and sugarcane bagasse. Chemical composition analysis showed total carbohydrate and lignin contents (% dry weight) of 69.6 ± 1.2 and 13.9 ± 0.4 for cassava peels and 70.3 ± 1.9 and 16.2 ± 1.2 for sugarcane bagasse, respectively. The feedstocks were subjected to acid, alkali and steam explosion pretreatments to increase cellulose content and therefore, reduce lignin content. The best pretreatment methods (steam explosion for sugarcane bagasse and acid for cassava peels) increased total carbohydrate contents to 85.4 ± 2.33 and 80.4 ± 2.5 for sugarcane bagasse and cassava peels, respectively. The respective lignin contents after pretreatment were 4.2 ± 0.44 and 4.8 ± 0.8 for sugarcane bagasse and cassava peels. Cultural conditions (pH, temperature, nitrogen source, inoculum size and substrate concentration) of the bacterium were optimized to enhance cellulase production. The laboratory scale fermentation of the feedstocks to ethanol was carried out in 250 mL Erlenmeyer flasks. Gas Chromatography -Mass spectrometry (GC-MS) analysis of the fermentation broth of sugarcane bagasse and cassava peels substrates revealed ethanol contents of 18.40 and 17.80 g/L, respectively. The study has demonstrated efficient bioethanol production by Bacillus cereus GBPS9 using sugarcane bagasse and cassava peels as feedstocks.

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Section: Discussionmentioning

confidence: 99%

Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks

Ezebuiro¹,

Ogugbue²,

Oruwari³

et al. 2015

J Biotechnol Biomater

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“…Bakteri selulolitik dan enzim selulasenya banyak dimanfaatkan untuk berbagai macam keperluan industri karena biaya produksinya murah, waktu produksi singkat, menghasilkan kompleks multienzim dan cenderung stabil pada kondisi ekstrem (Ladeira, et al, 2015). Selulase banyak digunakan dalam industri pulp dan kertas untuk modifikasi serat, penghilangan warna dan peningkatan drainase (Ladeira, et al, 2015), industri tekstil sebagai biopolishing kain dan deterjen yang digunakan untuk meningkatkan kelembutan dan kecerahan kain (Shadu & Maiti, 2013) serta produksi biofuel (Bogati, 2011).…”

Section: Seleksi Dan Identifikasi Bakteri Selulolitik Pendegradasi Daunclassified

“…Selulase banyak digunakan dalam industri pulp dan kertas untuk modifikasi serat, penghilangan warna dan peningkatan drainase (Ladeira, et al, 2015), industri tekstil sebagai biopolishing kain dan deterjen yang digunakan untuk meningkatkan kelembutan dan kecerahan kain (Shadu & Maiti, 2013) serta produksi biofuel (Bogati, 2011). Aplikasi selulase di bidang peternakan telah dilakukan untuk peningkatan kualitas bahan pakan ternak.…”

Section: Seleksi Dan Identifikasi Bakteri Selulolitik Pendegradasi Daunclassified

“…Chang et al (2009) menemukan bahwa Bacillus sp yang diisolasi dari kompos limbah Brassica memiliki aktivitas selulase berkisar antara 1,9-2,33 U/ml. Demikian pula Pandey et al (2014) dan Ladeira et al (2015) Bacillus clausii adalah bakteri berbentuk batang, merupakan bakteri Gram posit if , m otil dan membentuk endospora (Hema & Shiny, 2012). Bakteri ini dikenal memiliki potensi sebagai bakteri probiotik (Sun et al, 2010) karena memiliki aktivitas antibakteri (Bouhss et al, 2009) serta dapat memproduksi enzim penting seperti alkalin serin protease (Dilek et al, 2009), xilanase dan selulase (Ochieng, 2014).…”

Section: Figure 4 the Morphology Of Ug3 (Left) Ug7 (Right) And Ug8 unclassified

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Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

Mulyasari

Widanarni

Suprayudi

et al. 2015

JPBKP

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ABSTRAKPemanfaatan daun singkong sebagai bahan baku pakan ikan belum dilaksanakan secara optimal karena mengandung selulosa yang cukup tinggi. Tuj uan penelitian ini adalah mendapatkan bakteri yang dapat mendegradasi serat daun singkong dan melakukan identifikasi jenis bakteri selulolitik tersebut. Seleksi bakteri selulolitik dilakukan pada 4 jenis isolat bakteri asal saluran pencernaan ikan gurame yaitu UG3, UG6, UG7, dan UG8 dengan mengukur aktivitas enzim selulase secara kuantitatif pada substrat Carboxy Methyl Cellulose (CMC) dan daun singkong menggunakan metode 3,5-dinitrosalicylic acid (DNS). Identifikasi bakteri dilakukan pada 3 isolat yang memiliki aktivitas enzim selulase tertinggi pada substrat daun singkong berdasarkan morfologi (perwarnaan Gram, motilitas dan bentuk bakteri), uji biokimia (oksidase dan katalase) dan analisis gen (16S-rRNA). Hasil penelitian menunjukkan bahwa dari 4 isolat yang diuji cobakan, isolat UG7 memiliki aktivitas enzim selulase (CMCase) tertinggi (0,0043 U/ ml) sedangkan yang terendah adalah UG3 (0,0018 U/ml). Pada uji coba di substrat daun singkong, aktivitas enzim tertinggi terlihat pada isolat UG3 (0,107 U/ml) dan terendah adalah UG6 (0,077 U/ ml). Hasil identifikasi dari 3 jenis isolat bakteri uji yang memiliki kemampuan mendegradasi daun singkong tertinggi (UG3, UG7, dan UG8) menunjukkan bahwa ketiga jenis isolat tersebut merupakan bakteri jenis Gram positif berbentuk batang (basil). Berdasarkan data base dari GenBank, isolat UG3 memiliki kemiripan sebesar 93% dengan Bacillus clausii, UG7 memiliki kemiripan sebesar 96% dengan Bacillus amyloliquefaciens dan UG8 memiliki kemiripan sebesar 88% dengan Bacillus subtilis.

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“…The stability of the enzyme was studied by pre-incubating the enzyme in different buffers at different pH for 30 min at 37°C. Similarly, the effect of temperature on enzyme activity was determined by incubating the reaction mixture at different temperatures (30-90°C) for 30 min and activity was assayed (Ladeira et al, 2015). The temperature stability was determined by measuring the residual activity of the enzyme after incubating the enzyme at various temperatures.…”

Section: Purification and Characterization Of Cellulasementioning

confidence: 99%

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Kumaran¹,

Kalaichelv²,

Santhi³

2015

Microbiology J.

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The aim of the present investigation was to exploit the cheap agro-waste, sugarcane bagasse as a substrate for cellulase enzyme production using Bacillus licheniformis MTCC 429. The cellulase producing ability of B. licheniformis MTCC 429 was assessed using CMC agar plates. The factors affecting cellulase production were optimized by varying the parameters of incubation period, temperature and pH. The maximum cellulase enzyme production was achieved when the production medium pH was maintained at pH 7.0, temperature of 35°C with an incubation time of 48 h. Among the different concentration of sugarcane bagasse hydrolysate tested, CMC medium amended with 5% seemed to exhibit maximum cellulase productivity. Further, the partially purified enzyme was tested for its pH and temperature stability. The properties presented by B. licheniformis MTCC 429 suggest that the strain showed enhanced production of lignocellulosedegrading cellulase enzyme under the optimized conditions. The temperature and pH stability of the enzyme suggested its potential for industrial applications involving elevated conditions.

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Cellulase production by thermophilic Bacillus sp. SMIA-2 and its detergent compatibility

Cited by 126 publications

References 26 publications

Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks

Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

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