Cellulolytic activity and structure of symbiotic bacteria in locust guts

Su, Lihe; Liu, H.; Li, Y.; Zhang, H.-F.; Chen, M.; Gao, Xiaowei; Wang, F.-Q.; Song, Andong

doi:10.4238/2014.september.29.6

Cited by 17 publications

(9 citation statements)

References 10 publications

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“…Similarly, San et al (2011) also identified some other bacteria related to nitrogen metabolism, including Staphylococcus, Stenotrophomonas, etc. However, it cannot be ignored that this study is consistent with previous studies, that is, grasshoppers have a strong ability to digest cellulose (Su et al, 2014), how much of which is due to the action of their own digestive enzymes and how much of which is due to the contribution of microorganisms needs to be further explored.…”

Section: Discussionsupporting

confidence: 87%

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Wang¹,

Bai²,

Zheng³

et al. 2020

PeerJ

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Background Grasshoppers are typical phytophagous pests, and they have large appetites with high utilization of plants fibers, the digestion of which may depend on the microorganisms in their intestines. Grasshoppers have the potential to be utilized in bioreactors, which could improve straw utilization efficiency in the future. In this study, we describe the gut microbiome in three species of grasshoppers, Oedaleus decorus asiaticus, Aiolopus tamulus and Shirakiacris shirakii, by constructing a 16S rDNA gene library and analyzed the digestibility of cellulose and hemicellulose in the grasshoppers by using moss black phenol colorimetry and anthrone colorimetry. Results There were 509,436 bacterial OTUs (Operational Taxonomic Units) detected in the guts of all the grasshoppers sampled. Among them, Proteobacteria and Firmicutes were the most common, Aiolopus tamulus had the highest bacterial diversity, and Shirakiacris shirakii had the highest bacterial species richness. The intestinal microflora structure varied between the different species of grasshopper, with Aiolopus tamulus and Shirakiacris shirakii being the most similar. Meanwhile, the time at which grasshopper specimens were collected also led to changes in the intestinal microflora structure in the same species of grasshoppers. Klebsiella may form the core elements of the microflora in the grasshopper intestinal tract. The digestibility of cellulose/hemicellulose among the three species grasshoppers varied (38.01/24.99%, 43.95/17.21% and 44.12/47.62%). LEfSe analysis and Spearman correlation coefficients showed that the hemicellulosic digestibility of Shirakiacris shirakii was significantly higher than that of the other two species of grasshopper, which may be related to the presence of Pseudomonas, Stenotrophomonas, Glutamicibacter, Corynebacterium, and Brachybacterium in Shirakiacris shirakii intestinal tract. Conclusion The intestinal microbial communities of the three grasshoppers species are similar on phylum level, but the dominant genera of different species grasshoppers are different. The cellulose digestibility of the three species of grasshoppers is relatively high, which may be correlated with the presence of some gut microbiome. Increasing the understanding of the structure and function of the grasshopper intestinal microflora will facilitate further research and the utilization of intestinal microorganisms in the future.

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Section: Discussionsupporting

confidence: 87%

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Wang¹,

Bai²,

Zheng³

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…Similarly, San et al (2011)also identified some other bacteria related to nitrogen metabolism, including Staphylococcus, Stenotrophomonas, etc (San et al, 2011). However, it cannot be ignored that this study is consistent with previous studies, i.e., grasshoppers have a strong ability to digest cellulose (Su et al, 2014), how much of which is due to the action of their own digestive enzymes and how much of which is due to the contribution of microorganisms needs to be further explored.…”

Section: Discussionsupporting

confidence: 88%

Peer Review #1 of "Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility (v0.1)"

2020

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“…Cellulose‐degrading microorganisms have been isolated from the digestive tracts of insects that feed on diets rich in cellulosic substrates, such as Holotrichia parallela Motschulsky (Sheng et al, 2012), Diatraea saccharalis Fabr. (Dantur et al, 2015), Dendroctonus rhizophagus Thomas and Bright (Briones‐Roblero et al, 2017; Morales‐Jiménez et al, 2012), Lepidiota mansueta Burmeister (Handique et al, 2017), R. ferrugineus Olivier (Muhammad et al, 2017), Zyngetoma (Pothula et al, 2019), grasshoppers (Su et al, 2014), and wood‐eating insects, such as termites (Tsegaye et al, 2019; Upadhyaya et al, 2012; Wenzel et al, 2002) and cockroaches (Gijzen et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

Characterization of cultivable intestinal microbiota in Rhynchophorus palmarum Linnaeus (Coleoptera: Curculionidae) and determination of its cellulolytic activity

Calumby

Almeida

Barros

et al. 2022

Arch Insect Biochem Physiol

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Rhynchophorus palmarum Linnaeus is an agricultural pest that affects various palm crops, including coconut (Cocos nucifera) plantations which are prominent in the economy of Northeastern Brazil. Characterization of the intestinal microbiota of R. palmarum, as well as elucidation of aspects related to the biochemistry and physiology of the insect's digestion, is essential for intervention in specific metabolic processes as a form of pest control. Thus, this study aimed to characterize the intestinal microbiota of R. palmarum and investigate its ability to degrade cellulosic substrates, to explore new biological control measures. Intestinal dissection of eight adult R. palmarum insects was performed in a laminar flow chamber, and the intestines were homogenized in sterile phosphate‐buffered saline solution. Subsequently, serial dilution aliquots of these solutions were spread on nutritive agar plates for the isolation of bacteria and fungi. The microorganisms were identified by matrix‐assisted laser desorption/ionization with a time‐of‐flight mass spectrometry and evaluated for their ability to degrade cellulose. Fourteen bacterial genera (Acinetobacter, Alcaligenes, Arthrobacter, Bacillus, Citrobacter, Enterococcus, Kerstersia, Lactococcus, Micrococcus, Proteus, Providencia, Pseudomonas, Serratia, and Staphylococcus) and two fungal genera (Candida and Saccharomyces)—assigned to the Firmicutes, Actinobacteria, Proteobacteria, and Ascomycota phyla—were identified. The cellulolytic activity was exhibited by six bacterial and one fungal species; of these, Bacillus cereus demonstrated the highest enzyme synthesis (enzymatic index = 4.6). This is the first study characterizing the R. palmarum intestinal microbiota, opening new perspectives for the development of strategies for the biological control of this insect.

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Cellulolytic activity and structure of symbiotic bacteria in locust guts

Cited by 17 publications

References 10 publications

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Peer Review #1 of "Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility (v0.1)"

Characterization of cultivable intestinal microbiota in Rhynchophorus palmarum Linnaeus (Coleoptera: Curculionidae) and determination of its cellulolytic activity

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