Cellulose acetate/poly lactic acid coaxial wet-electrospun scaffold containing citalopram-loaded gelatin nanocarriers for neural tissue engineering applications

Naseri-Nosar, Mahdi; Salehi, Majid; Hojjati-Emami, Shahriar

doi:10.1016/j.ijbiomac.2017.05.054

Cited by 90 publications

(41 citation statements)

References 42 publications

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“…At the end of 14th week postsurgery, the animals were sacrificed and the posterior gastrocnemius muscles on the injured and uninjured hind limbs were harvested and immediately weighed to determine the wet weight‐loss of gastrocnemius muscles using the following equation

Gastrocnemius muscle wet weight - loss true(% true) = (1 - \frac{Wet weight of the muscle on the injured side}{Wet weight of the muscle on the uninjured side}) \times 100

…”

Section: Methodsmentioning

confidence: 99%

Sciatic nerve regeneration by transplantation of Schwann cells via erythropoietin controlled‐releasing polylactic acid/multiwalled carbon nanotubes/gelatin nanofibrils neural guidance conduit

et al. 2017

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The current study aimed to enhance the efficacy of peripheral nerve regeneration using an electrically conductive biodegradable porous neural guidance conduit for transplantation of allogeneic Schwann cells (SCs). The conduit was produced from polylactic acid (PLA), multiwalled carbon nanotubes (MWCNTs), and gelatin nanofibrils (GNFs) coated with the recombinant human erythropoietin-loaded chitosan nanoparticles (rhEpo-CNPs). The PLA/MWCNTs/GNFs/rhEpo-CNPs conduit had the porosity of 85.78 ± 0.70%, the contact angle of 77.65 ± 1.91° and the ultimate tensile strength and compressive modulus of 5.51 ± 0.13 MPa and 2.66 ± 0.34 MPa, respectively. The conduit showed the electrical conductivity of 0.32 S cm and lost about 11% of its weight after 60 days in normal saline. The produced conduit was able to release the rhEpo for at least 2 weeks and exhibited favorable cytocompatibility towards SCs. For functional analysis, the conduit was seeded with 1.5 × 10 SCs and implanted into a 10 mm sciatic nerve defect of Wistar rat. After 14 weeks, the results of sciatic functional index, hot plate latency, compound muscle action potential amplitude, weight-loss percentage of wet gastrocnemius muscle and Histopathological examination using hematoxylin-eosin and Luxol fast blue staining demonstrated that the produced conduit had comparable nerve regeneration to the autograft, as the gold standard to bridge the nerve gaps. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1463-1476, 2018.

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Gastrocnemius muscle wet weight - loss true(% true) = (1 - \frac{Wet weight of the muscle on the injured side}{Wet weight of the muscle on the uninjured side}) \times 100

…”

Section: Methodsmentioning

confidence: 99%

Sciatic nerve regeneration by transplantation of Schwann cells via erythropoietin controlled‐releasing polylactic acid/multiwalled carbon nanotubes/gelatin nanofibrils neural guidance conduit

et al. 2017

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“…Collectively, these data indicate that MC is well suited as a biocompatible injectable scaffold for the repair of brain defects [241][242][243]. Gelatin coated nanoparticles contained in cellulose acetate/PLA scaffolds showed higher cell viability than uncoated scaffolds, and they acted as a nerve guidance conduit for sciatic nerve defects in vitro and in vivo [244], while a gelatin/chitosan/PEDOT hybrid scaffold enhanced the neurite growth of PC12 cells and promoted neuron-like cell adhesion and proliferation [245].…”

Section: Cellulosementioning

confidence: 91%

Piezoelectric Scaffolds as Smart Materials for Neural Tissue Engineering

2020

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Injury to the central or peripheral nervous systems leads to the loss of cognitive and/or sensorimotor capabilities, which still lacks an effective treatment. Tissue engineering in the post-injury brain represents a promising option for cellular replacement and rescue, providing a cell scaffold for either transplanted or resident cells. Tissue engineering relies on scaffolds for supporting cell differentiation and growth with recent emphasis on stimuli responsive scaffolds, sometimes called smart scaffolds. One of the representatives of this material group is piezoelectric scaffolds, being able to generate electrical charges under mechanical stimulation, which creates a real prospect for using such scaffolds in non-invasive therapy of neural tissue. This paper summarizes the recent knowledge on piezoelectric materials used for tissue engineering, especially neural tissue engineering. The most used materials for tissue engineering strategies are reported together with the main achievements, challenges, and future needs for research and actual therapies. This review provides thus a compilation of the most relevant results and strategies and serves as a starting point for novel research pathways in the most relevant and challenging open questions.

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“…Recently, gelatin nanoparticles have been used to enhance the biocompatibility of polymeric scaffolds for neural tissue engineering. For example, gelatin coated nanoparticles contained in cellulose acetate/PLA scaffolds showcased higher cell viability than uncoated scaffolds and they acted as a nerve guidance conduit for sciatic nerve defects in vitro and in vivo [75] while a gelatin/chitosan/PEDOT hybrid scaffold enhanced neurite growth of PC12 cells and promoted neuron-like cell adhesion and proliferation [76].…”

Section: Natural Polymers For Neural Tissue Engineeringmentioning

confidence: 99%

Current and novel polymeric biomaterials for neural tissue engineering

et al. 2018

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The nervous system is a crucial component of the body and damages to this system, either by of injury or disease, can result in serious or potentially lethal consequences. Restoring the damaged nervous system is a great challenge due to the complex physiology system and limited regenerative capacity.Polymers, either synthetic or natural in origin, have been extensively evaluated as a solution for restoring functions in damaged neural tissues. Polymers offer a wide range of versatility, in particular regarding shape and mechanical characteristics, and their biocompatibility is unmatched by other biomaterials, such as metals and ceramics. Several studies have shown that polymers can be shaped into suitable support structures, including nerve conduits, scaffolds, and electrospun matrices, capable of improving the regeneration of damaged neural tissues. In general, natural polymers offer the advantage of better biocompatibility and bioactivity, while synthetic or non-natural polymers have better mechanical properties and structural stability. Often, combinations of the two allow for the development of polymeric conduits able to mimic the native physiological environment of healthy neural tissues and, consequently, regulate cell behaviour and support the regeneration of injured nervous tissues.Currently, most of neural tissue engineering applications are in pre-clinical study, in particular for use in the central nervous system, however collagen polymer conduits aimed at regeneration of peripheral nerves have already been successfully tested in clinical trials.This review highlights different types of natural and synthetic polymers used in neural tissue engineering and their advantages and disadvantages for neural regeneration.

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Cellulose acetate/poly lactic acid coaxial wet-electrospun scaffold containing citalopram-loaded gelatin nanocarriers for neural tissue engineering applications

Cited by 90 publications

References 42 publications

Sciatic nerve regeneration by transplantation of Schwann cells via erythropoietin controlled‐releasing polylactic acid/multiwalled carbon nanotubes/gelatin nanofibrils neural guidance conduit

Sciatic nerve regeneration by transplantation of Schwann cells via erythropoietin controlled‐releasing polylactic acid/multiwalled carbon nanotubes/gelatin nanofibrils neural guidance conduit

Piezoelectric Scaffolds as Smart Materials for Neural Tissue Engineering

Current and novel polymeric biomaterials for neural tissue engineering

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