Cellulose conversion in dry grind ethanol plants

Ladisch, Michael R.; Dale, Bruce E.; Tyner, Wally; Mosier, Nathan S.; Kim, Youngmi; Cotta, Michael A.; Dien, Bruce S.; Blaschek, Hans P.; Laurenas, Edmund; Shanks, Brent H.; Verkade, John G.; Schell, C.; Petersen, Gene R.

doi:10.1016/j.biortech.2007.09.082

Cited by 15 publications

(5 citation statements)

References 10 publications

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“…As expected, Tables II and III show that conversion of cellulose into water‐soluble oligosaccharides and monosaccharides is minimal, and that formation of aldehydes and other sugar degradation products is negligible when these tissues are pretreated with liquid hot water at 190°C for 15 min, at a pH of 4–7 with a 5 min heat‐up time (Ladisch et al, 2008). The effect of pretreatment is as represented by Equation (2).…”

Section: Resultssupporting

confidence: 53%

Tissue‐specific biomass recalcitrance in corn stover pretreated with liquid hot‐water: Enzymatic hydrolysis (part 1)

Zeng

Ximenes

Ladisch

et al. 2011

Biotech & Bioengineering

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Lignin content, composition, distribution as well as cell wall thickness, structures, and type of tissue have a measurable effect on enzymatic hydrolysis of cellulose in lignocellulosic feedstocks. The first part of our work combined compositional analysis, pretreatment and enzyme hydrolysis for fractionated pith, rind, and leaf tissues from a hybrid stay-green corn, in order to identify the role of structural characteristics on enzyme hydrolysis of cell walls. The extent of enzyme hydrolysis follows the sequence rind < leaves < pith with 90% conversion of cellulose to glucose in 24 h in the best cases. Physical fractionation of corn stalks or other C(4) grasses into soft and hard tissue types could reduce cost of cellulose conversion by enabling reduced enzyme loadings to hydrolyze soft tissue, and directing the hard tissue to other uses such as thermal processing, combustion, or recycle to the land from which the corn was harvested.

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Section: Resultssupporting

confidence: 53%

Tissue‐specific biomass recalcitrance in corn stover pretreated with liquid hot‐water: Enzymatic hydrolysis (part 1)

Zeng

Ximenes

Ladisch

et al. 2011

Biotech & Bioengineering

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“…Recent research has also explored hot water pretreatment (LHW) and ammonia fiber expansion pretreatment (AFEX) methods prior to the enzyme hydrolysis of distillers' grains [16][17][18][19]. Both pretreatment methods were effective in enhancing the digestibility of the distillers' grains and minimizing the formation of monomeric sugars (MS) prior to the enzyme hydrolysis step.…”

Section: Introductionmentioning

confidence: 99%

Stagewise Dilute-Acid Pretreatment and Enzyme Hydrolysis of Distillers’ Grains and Corn Fiber

Noureddini

Byun

2009

Appl Biochem Biotechnol

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Distillers' grains and corn fiber are the coproducts of the corn dry grind and wet milling industries, respectively. Availability of distillers' grains and corn fiber at the ethanol plant and their high levels of lignocellulosic material make these coproducts attractive feedstocks for conversion to ethanol. In this study, dilute sulfuric acid hydrolysis of these coproducts was investigated in a multistage scheme. After the completion of each pretreatment stage, the liquid substrate was separated and reused in the succeeding pretreatment stage with a fresh substrate. The substrate from each stage was also subjected to enzyme hydrolysis in a separate experiment. The sulfuric acid concentration and the substrate loading were maintained at 1.0 vol% and 15.0 wt.%, respectively, and the temperature was maintained at 120 degrees C in all the experiments. Experiments were also performed to study the effect of removing oil from the samples prior to the pretreatment. The highest concentration of monomeric sugars (MS) was observed when three stages of pretreatment were followed by the enzyme reaction. The enzyme hydrolysis of the three-stage pretreated dried distillers' grains and corn fiber yielded 122.6 +/- 5.8 and 184.5 +/- 4.1 mg/mL of MS, respectively. The formation of inhibitory products was also monitored.

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“…The structural composition of rice husk is 34 % Glucan, 15 % Xylan, 17% Lignin, 11% ash, and 2.2% Arabinan. Nondigestible oligosaccharides (NDO) and Fermentable sugar can be produced by using it as raw material [9]. The increasing application of XOS in cosmetics, pharmaceutical, and medical contributed emerging modern technologies for the production of XOS.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of rice husk as a substrate for the growth of Lactobacillus species

Palwasha

2025

PAB

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The most produced and consumed cereal is Rice (Oryza sativa). Rice husk is composed of 11% hemicellulose with xylan being the main sugar subunit. Xylan is the most abundant carbohydrate in lignocellulosic biomass the precursor of Xylooligosaccharides (XOS), used as prebiotics. Toxic products and activity of pathogenic bacteria are decreased by xylooligosaccharides which enables the growth of probiotic bacteria. The present study was aimed to study the by-products of rice cultivation as a substrate for the growth of Lactobacillus species. The collected rice husk was weighed and dried in Hot Air Oven. After hot air drying the sample was weighed again and measured the dry weight. A two-step auto-hydrolysis method was used. The liquor contained xylooligosaccharides which were refined by filtration. The non-digestible oligosaccharides XOS were predominant in acid extract when sulfuric acid was 2% (w/w), temperature and time were 100°C and 0.5 hours respectively. The prebiotic efficacy of XOS was checked with Lactobacillus strains grown in MRS broth for in-vitro evaluation. The OD600 was high with the liquor extract of rice husk instead of other sugar. This study shows xylan from agriculture waste produces XOS, an emerging prebiotic with a promising low-cost strategy of production.

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Cellulose conversion in dry grind ethanol plants

Cited by 15 publications

References 10 publications

Tissue‐specific biomass recalcitrance in corn stover pretreated with liquid hot‐water: Enzymatic hydrolysis (part 1)

Tissue‐specific biomass recalcitrance in corn stover pretreated with liquid hot‐water: Enzymatic hydrolysis (part 1)

Stagewise Dilute-Acid Pretreatment and Enzyme Hydrolysis of Distillers’ Grains and Corn Fiber

Evaluation of rice husk as a substrate for the growth of Lactobacillus species

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